You digest 4 uL of plasmid DNA that is 50 ng/uL concetration in a total volume of 20 uL. You run 10 uL of the digest on teh gel. You then do a DNA purification protocol with a Zippy prep on the remaining digested DNA. You elute the DNA in a 25 uL. A 2 uL ssample has the concentration of 2 ng/uL. What is the DNA yield?

Why is this the only correct answer? Why doesn't carbonic anhydrase function the best at pH 7?

When you are loading the DNA ladder into your gel the volume needed is 5uL. The appropriate micropipette for this volume is a: 1)p2 2)p200 3)p1000 4)p20

What would spread (fragmented) bands in the electrophoresis gel tell you about the quality of your DNA? Explain briefly, yet clearly

1) Prepare the following enzymatic reaction, present it in tabulated form. In a final volume of 30 ul, where buffer 4 (10 ml). How much volume of each reagent would be used and how much of water? Is there any problem? 2) The DNA pol 1 enzyme comes at a concentration of 50,000 U/ml. You have to prepare a 50 ug PCR reaction where you must use 0.05 U/ml reaction. You add 10 ul of PCR buffer, 2 ng of tempered DNA that is at a concentration of 0.5 ng/ul, primers (which are at 200 mM) so that each one remains at a concentration of 200 uM, Mg+2 that is 5 mM (10 X), enzyme and water. Present the table of all the reagents included in the reaction, the volumes of each one in ul. Present where the initial and final concentration of each reagent applies. Assume you have micropipettes for all values.

How many microliters of pGLO plasmid will you need for a PCR reaction is you need 200 nanograms and the plasmid concentration is 0.05 micrograms/microliter?

After plasmid isolation, you take 0.5 uL of the sample and mix it with 1 uL of TE buffer. The absorbance of this sample at 260 nm is 0.8 and the average extinction coefficient of double-stranded DNA at 260 nm is 0.020 (μg/ml)−1 cm−1. What is the concentration of the undiluted sample?     120 μg/mL     80 μg/mL     40 μg/mL     12 μg/mL

What is the purpose of using the Chelex beads while extracting the DNA at high temperature from your cheek cells? Group of answer choices   The Chelex binds cations like Mg2+ that are co-factors for nuclease enzymes which would otherwise degrade the DNA.   The Chelex denatures the DNA and makes the DNA single stranded.   The Chelex will bind the DNA tightly after the cells are ruptured, helping extract it from the cells.   The Chelex provides Mg2+ co-factors needed by DNA polymerase enzymes to make more copies of DNA

Use the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy.   What is the smallest fragment size that the NcoI/EcoRI double digest produces?

Which of the following gels is the preferred choice for separation of DNA fragments? Agarose Polyacrylamide SDS PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis) Agar