The special strain of Neurospora that makes citrulline but not arginine and the mutant or missing gene in the strain. Introduction. All the enzymes in the body that are crucial for the biochemical reactions are proteins. The proteins are made of amino acids. The amino acids are of 20 types that combine in a varied manner to form proteins. The amino acids join together by peptide bonds. Proteins act as major substrates and reactants for the metabolic pathways.

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Chapter 1, Problem 4P

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To determine: The special strain of Neurospora that makes citrulline but not arginine and the mutant or missing gene in the strain.

Introduction. All the enzymes in the body that are crucial for the biochemical reactions are proteins. The proteins are made of amino acids. The amino acids are of 20 types that combine in a varied manner to form proteins. The amino acids join together by peptide bonds. Proteins act as major substrates and reactants for the metabolic pathways.

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The figure below shows the life cycle of the fungus Neurospora. The adult stage of the Neurospora is a multicellular haploid. b) Neurospora has an arginine amino acid synthesis pathway shown below. Suppose I take the strain above that only grows with arginine supplements and cross it to a different mutant Neurospora strain that grows with arginine and citrulline supplements but not with ornithine supplements. Assuming gens A, B, and C are unlinked and there is only one mutation per stain: What percentage of the progeny will grow on ornithine? What percentage on citrulline? What percentage on arginine?

A gene contains the sequence CGCATACGGTAC that results in the amino acid sequence arg-ile-arq- tyr. A mutation in this gene has a G inserted after the second C in the strand. How will this mutation affect the phenotype? A)This will affect the phenotype because although most of the protein will be identical, the first amino acid will be different. B)This will not affect the phenotype because only the second amino acid is different from the original protein. C)This will not affect the phenotype because the protein will be identical to the original protein. D)This will affect the phenotype because all of the amino acids after the first one will be different from the original protein.

Neurospora crassa can synthesize the amino acid arginine from a precursor as follows: precursor ornithine citrulline arginine George Beadle and Edward Tatum identified various mutants (arg mutants) unable to synthesize arginine from this precursor. One such class (Class I) could synthesize arginine if either ornithine or citrulline was supplied. A second mutant class (Class II) could synthesize arginine if citrulline was added, but not if ornithine was added. A third class (Class III) could not synthesize arginine from either ornithine or citrulline. Which class or classes of arg mutants will grow on complete media?