QUESTION 2You obtained the following raw data when setting up a Bradford standard curve:01234BSA (mg/ml) 595nmAbsorbancy6789100.2250.3100.4200.5100.6100.7200.8100.9150.9500.9800.990After blanking against a bradford-dH₂O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of0.568 was obtained.Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3significant figures).

Bradford assay is a quantitative method to determine the total protein concentration in any sample…

Answered: You obtained the following raw data…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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168 56572 w11o QUESTION 2 You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0.225 0.310 0.420 0.510 4. 0.610 0.720 0.810 0.915 0.950 0.980 0.990 After blanking against a bradford-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.679 was obtained. Set up a standard curve, ekeluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
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