The normal freezing point of benzene (C6H6) is 5.5 °C. If 30.92 grams of the nonvolatile nonelectrolyte diphenylether (C12H100), are dissolved in 212.1 grams of benzene, what is the freezing point of the resulting solution? Kfp for benzene is 5.12 °C/m. °C
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- Quinine ( C20 H24 N2 O2) is the most important alkaloid derived from cinchona bark. It is used as an antimalarial drug. For quinine, pK, = 5.1 and pK, = 9.7 ( pKp = – log Kp). Only 1 g quinine will dissolve in 1920.0 mL of solution. Calculate the pH of a saturated aqueous solution of quinine. Consider only the reaction | Q+ H2O= QH+ + OH- described by pK, where Q = quinine. pH =200 microliters of a standard solution of 0.200 mg/mL caffeine was mixed with 4.8 mL of 50 mM sodium phosphate buffer, pH 3: methanol (60:40 v/v). What is the concentration of caffeine, in mg/mL, in this solution? Give your answer to two significant figures.The isoelectric point of eIF4a is 5.02. Students are given a sample of cell lysate containing eIF4A at pH 7.4. They are also provided with two buffers of pH 7.4 to use for ion exchange chromatography. One buffer has a very high salt concentration (1 M), the other has a low salt concentration (0.1 M). Mariela decides to use an anion exchange column, while Ashok chooses a cation exchange column. Who made the better decision? Provide a detailed explanation of why one student will end up with a purer sample of eIF4A. Which buffer will you use while washing away impurities (high or low salt), and which would you use to remove eIF4A from the column? Explain your choice.
- What mass of Na2SO4 must be dissolved in 75.0 grams of water to lower the freezing point by 2.50 °C? The freezing point depression constant of water is 1.86 K kg mol¹. Assume ideal behaviour for Na2SO4 in solution.What mass of sodium glycolate (NaC2H3O3) should be added to 400.0 mL of 1.00 M glycolic acid to produce a buffer solution with a pH of 4.00? Ka = 1.47 x 10-4. Please indicate the full solutions.Predict the diffusivity of human serum albumin at 293 K in water as a dilute solution and compare it with experimental data. Assume the following: The molecular weight of human serum albumin, MA= 72,300 kg/kmol The viscosity of water at 293 K is 0.897 x 10-3 Pa∙s The experimental data value is 5.93 x 10-11 m2/s.
- Consider a mixture of two proteins with molecular weights of 20,000 and 200,000. For simplicity of calculation, both may be approximated as unhydrated spheres with VB 0.740 mL g1. a. Calculate the ratio of the sedimentation coefficients of the proteins in the same medium (density=1.05 g mL¯1) at the same temperature.Thimerosal Tincture USP contains 0.1% w/v thimerosal and 50% v/v ethyl alcohol. If the cap is left off of a 15-mL bottle of the tincture, and the ethyl alcohol evaporates leaving a final volume of 9.5 mL, what is the concentration of thimerosal in the evaporated solution expressed as a ratio strength?What mass of sodium glycolate (NaC2H3O3) should be added to 400.0 mL of 1.00 M glycolic acid to produce a buffer solution with a pH of 4.00? Ka = 1.47 x 10-4
- Tirofiban (C22H36N₂O5S MWt = 440.6) is present as tirofiban HCI monohydrate (C22H36N2O5S.HCI.H₂O MWt=495.1) at 0.281 mg/mL in a concentrated solution. A solution for infusion is prepared by extracting 50 mL from a 250 mL bag of 5% glucose solution and adding 50 mL of concentrated solution. Sofia who weighs weighs 87 kg requires a tirofiban dose of 0.1 mcg/kg/min for 12 to 24 hours. What would the infusion rate be (mL/h)? (Answer to 1 decimal place.) Answer:What is the pH of a buffer solution that is 0.20 M proprionic acid (HC3H4O2) and 0.1 M sodium proprionate (NaC3H4O2)? The KA of proprionic acid is 1.3 x 10-5Assuming the molar extinction coefficient for para-nitrophenyl phosphate (PNP) is 55,290 mol "dm cm, calculate the concentration of the molecule that produced an absorbance of 0.770 OD units in a 1.5 cm cuvette. Show all working -1 3 -1