Draw the Mchelis Menton plot and the Line Weaver Burk Plot using the data given below and find the Vmax and Km from the Plot. AmountVelocity [Vo] inumoles/min/mlof[s] inmilligrams[S] in %1/[S]OD at 540nmmaltose1/Voin μmoles0.50.062340.137.60.21010.135040.283.50.1200.228640.482.080.053030.2911520.641.560.034040.3313140.731.360.0255050.3513860.771.290.02

Michaelis-Menton plot is drawn using Michaelis-Menton equation to determine Km and Vmax of the…

Amo Velocity [Vo] in umoles/min/ml of [s] in milligrams [S] in % OD at 540nm maltose 1/Vo 1/[S] in μmoles 0.5 0.06 234 0.13 7.6 0.2 10 1 0.13 504 0.28 3.5 0.1 20 0.22 864 0.48 2.08 0.05 30 3 0.29 1152 0.64 1.56 0.03 40 4 0.33 1314 0.73 1.36 0.025 50 5 0.35 1386 0.77 1.29 0.02

Amo Velocity [Vo] in umoles/min/ml of [s] in milligrams [S] in % OD at 540nm maltose 1/Vo 1/[S] in μmoles 0.5 0.06 234 0.13 7.6 0.2 10 1 0.13 504 0.28 3.5 0.1 20 0.22 864 0.48 2.08 0.05 30 3 0.29 1152 0.64 1.56 0.03 40 4 0.33 1314 0.73 1.36 0.025 50 5 0.35 1386 0.77 1.29 0.02

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

Draw the Mchelis Menton plot and the Line Weaver Burk Plot using the data given below and find the Vmax and Km from the Plot.

Amount
Velocity [Vo] in
umoles/min/ml
of
[s] in
milligrams
[S] in %
1/[S]
OD at 540nm
maltose
1/Vo
in μmoles
0.5
0.06
234
0.13
7.6
0.2
10
1
0.13
504
0.28
3.5
0.1
20
0.22
864
0.48
2.08
0.05
30
3
0.29
1152
0.64
1.56
0.03
40
4
0.33
1314
0.73
1.36
0.025
50
5
0.35
1386
0.77
1.29
0.02

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