How much of the enzyme proteinase k (solute) is required to make 250ml of a solution with a concentration of .01mg/ml? (Weight/volume ratio)

Q: Calculate the molarity (M) of a 46% (w/v) copper sulfate solution (m.w. = 160.0 g/mol)

A: Molarity is the number of moles of solute per litre of solution.

Q: Normal arterial pH is 7.35 to 7. 45. Both metabolic and respiratory alkalosis have pH more basic…

A: DISCLAIMER: Since you have asked multiple questions we will solve the first question for you. If you…

Q: The normal freezing point of benzene (C6H6) is 5.5 °C. If 30.92 grams of the nonvolatile…

A:

Q: A beaker with 1.40x102 mL of an acetic acid buffer with a pH of 5.000 is sitting on a benchtop. The…

A:

Q: What is the molarity of a 30% solution of NaCI

A: Let us first define the percentage solution : 30% NaCl solution= 30% w/v solution = 30g/100 ml.Since…

Q: You record the absorbance of your unknown BSA sample at 0.276. What is the concentration in ug/mL of…

A: Standard curve can be used to determine the concentration of unknown sample by determining their…

Q: You have a 62% dextrose solution. How many mL will give you 35 g of dextrose?

A: Carbohydrates are molecules that include carbon, hydrogen, and oxygen and have the formula (CH2O)n.…

Q: If an aqueous solution has a hydroxyl ion concentration of 1 x 10-6 M, what is the concentration of…

A: Water dissociates into hydrogen ions and hydroxyl ions and at neutral pH (pH =7) the numbers of…

Q: When 2.43 g of a nonelectrolyte solute is dissolved in water to make 435 mL of solution at 25 °C,…

A: The compounds which will not ionize in any solution are called nonelectrolytes. The electricity will…

Q: Explain how do you prepare a 25-mL solution of 1 mg/mL cholesterol stock?

A: Given Values: Volume = 25 ml Concentration = 1 mg/ml

Q: How much solute do you need to make 250 ml of a 3M stock of glucose?

A: The Molarity of a solution is the number of moles of a solute dissolved in a liter of solution. That…

Q: Provided Stock solution of Coomassie Blue: 0.500 mg/mL Volume of 0.500 mg/ml Solution Dilution to ID…

A: For calculating volume and concentration,  we should follow the formula V1S1 = V2S2where, V1 =…

Q: How many mL of the 1 M glucose stock solution do you need to prepare 100 mL of a 1 mM glucose…

A: After dilution, the dilution factor represents the number of times the original concentrated stock…

Q: Please show how the solution of the concentration (the highlighted color) of the unknown solution

A: As concentration increases the absorbance is also increasing. 50 ppm concentration  Absorbance is…

Q: What is the pH of a solution that has an H¯ concentration of 1.75 x 10° mol/L

A: pH is the measure of the acidity or basicity of a medium.  It is measured as the negative logarithm…

Q: If the pH of a voledronic acid solution is 5.8, and the voledronate concentration is 9 mM, what is…

A: Henderson Hasselbalch equation can be used to determine the concentration of a weak acid in an…

Q: How many grams of NaOH would you need to make 3,982 mL of a 3 M NAOH solution (m.w. = 40.00 g/mol)?…

A: By the concept of molarity, we can determine the concentration of a solution. It is the total number…

Q: A chemist is performing a precipitation titration on a 1.00 L sample containing an unknown…

A: Titration is the process, in which the exact concentration of the unknown solution can be used to…

Q: You are given two proteins with a pI of 4.5 and pI of 7.7. In an example of an anion-exchange…

A: Ion exchange chromatography is an isolation and purification technique used to separate biomolecules…

Q: What is the relationship betweenpKa and the useful range of a buffer?

A: The strength of an acid in a solution can be expressed in terms of the acid dissociation constant…

Q: You need to make a protein buffer of: 100 mM NaCl ⚫ 25 mM Tris 8 • • 5% w/v glycerol 2 mM DTT Your…

A: In order to calculate how much of each component we should use to get the required buffer, we need…

Q: 3 mL of a 45 mM stock solution of a substrate is added to 8 mL of water. Calculate the following…

A: Calculations for a substrate solution's dilution are related to the subject addressed above. A…

Q: The concentration of the conjugate base in 100 mL of 50 mM CH3COOH/CH3COO- (pKa = 4.76) at pH 5.00…

A: Step 1: Calculation of the concentration of the conjugate base (CH3COO⁻) at pH 5.00.The…

Q: You want 235mL of a solution that consists of 10% BSA, 2.37mM KCl and 120µM EDTA.  You have stock…

A: BSA is a globular proteins which is a well studied proteins and used as standard protein molecule in…

Q: It is recommended that children should have fluoride supple- ments for their teeth if the fluoride…

A: To convert parts per million (ppm) to micrograms per liter (µg/L), we need to know the molar mass of…

Q: You have a 100X stock of a buffer. You need 100 ml of 1X buffer.  How much buffer solution do you…

A: Stock solutions of the standard concentration are present in the laboratories. Using these stock…

Q: Your supervisor asks to make 350 mL of a 0.75% (w/v) starch solution using a 5% (w/v) stock…

A: Given Values: Concentration of the starch stock solution = 5 % The volume of the final starch…

Q: Calculate pH of buffer prepared by mixing; 10ml of 0.10M of CH3COOH and 20ml of 0.10M of CH3COONa.

A: A buffer is defined as the mixture of a weak acid and its conjugate base or weak base and its…

Q: 12 grams of glucose will make how many mL of a 0.5 g/mL solution?

A: Given Values: Weight of glucose = 12 g Concentration = 0.5 gml How much volume of water needs to…

Q: How much water would need to be added to 736 mL of a 3.15 M HCl solution to make a 0.78 M solution?

A:

Q: You are asked to prepare HEPES buffered saline with the following constituents: НЕРES 10mM KCI 5mM…

A: The concentration of a solution is expressed in terms of the ratio of the amount of solute present…

Q: To prepare 500 mL of a 1X TAE buffer solution, we need to mix solution (so 25 times more…

A: TAE is a standard buffer for the preparation and operation of DNA agarose gels. TBE provides…

Q: How do you make a stock solution of 1000 ppm using a solution which Has a density of 0.842 g/mL?

A: Introduction : To prepare a stock solution of 1000ppm using a solution with a density of 0.842g/𝑚L,…

Q: A 110.0value - mL buffer solution is 0.255M in NH3 and 0.395 M in NH4Br. The Kb alue for NH3 is 1.76…

A: Step 1: Given:Volume:  110 mL Concentration of NH3 = 0.255 MConcentration of NH4Br = 0.395 MKb of…

Q: For Sample 1, the glucose/fructose standard (0.2 mg/mL for each of glucose and fructose): From the…

A: Step 1:Step 2: Step 3: Step 4:

Q: Define the following: - pH - Buffer - pKa

A: An acid-base reaction takes place between an acid and a base and is used to determine pH. An acid is…

Q: B. You will need approximately 1 ml of the 100 µg/ml bromophenol blue (which is your most…

A: The stock concentration is 1mg/ml.Working solution concentration is 100 ug/ml. As both are two…

Q: Thimerosal Tincture USP contains 0.1% w/v thimerosal and 50% v/v ethyl alcohol. If the cap is left…

A: The objective of this question is to determine the concentration of thimerosal in the evaporated…

Q: You have been provided with stock solutions of: stock A:     0.06 M sodium pyrophosphate buffer pH…

A: Buffer preparation fro stock solution: The formula used for buffer preparation from stock solution…

Q: Calculate the volume of TAE buffer that you will need to prepare a 100ml solution of 1X strength…

A: TAE buffer stands for Tris-acetate-EDTA buffer. It is used in lab to do electrophoresis of nucleic…

Q: Which of the following combinations would be the best choice to buffer the pH of a solution at…

A: Ans- NaH2PO4 and Na2HPO4 would be the best choice to buffer the pH of a solution because it has pka…

Q: 2+ What is the Pb when 25.0mL of a 0.100MPbCl₂ (aq) 2 solution is added to 25.0mL of 0.150MCaCl ? K…

A: Write the balanced chemical equation.The reaction between CaCl2 and PbCl2 is a double displacement…

Q: What would be the working stock concentration (in molarity) if you diluted 250 uL of a stock…

A: Molarity is defined as the number of moles dissolves in 1000 ml of the solution.