can you show how to calculate the amount of two components for the required volume of a buffer with the receipes? Prepare enough 10X stock solution § Prepare 1L Proportions § 0.25M tris § 1.92M glycine § pH 8.3 § 1% (w/v) SDS Instructions to prepare 100mL of 10X running buffer 1. Weigh 3.02g of Tris base and 14.42g of glycine into ~90mL of ddH2O in a clean beaker and stir to dissolve. 2. Check the pH with a calibrated pH metre once dissolved. § The pH of the buffer should be 8.3. pH adjustment is not required if between 8.1-8.5. 3. Add 5mL 20% (w/v) SDS stock solution (a liquid) to make a 1% (w/v) solution. 4. Top the volume to 100mL by adding 5mL ddH2O from a graduated cylinder.

can you show how to calculate the amount of two components for the required volume of a buffer with the receipes? Prepare enough 10X stock solution § Prepare 1L Proportions § 0.25M tris § 1.92M glycine § pH 8.3 § 1% (w/v) SDS Instructions to prepare 100mL of 10X running buffer 1. Weigh 3.02g of Tris base and 14.42g of glycine into ~90mL of ddH2O in a clean beaker and stir to dissolve. 2. Check the pH with a calibrated pH metre once dissolved. § The pH of the buffer should be 8.3. pH adjustment is not required if between 8.1-8.5. 3. Add 5mL 20% (w/v) SDS stock solution (a liquid) to make a 1% (w/v) solution. 4. Top the volume to 100mL by adding 5mL ddH2O from a graduated cylinder.

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter17: Introduction To Iv Therapy

Section: Chapter Questions

Problem 3.6P

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