Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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The concentration of the conjugate base in 100 mL of 50 mM CH3COOH/CH3COO- (pKa = 4.76) at pH 5.00 is ______mM. If 100 μL of 1.0 M NaOH is added, the buffer pH is expected to shift to_______
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- Bonlyarrow_forwardThe concentration of the conjugate base in 100 mL of 50 mM CH3COOH/CH3COO- (pKa = 4.76) at pH 5.00 is ______mM. If 100 μL of 1.0 M NaOH is added, the buffer pH is expected to shift to ______ please answer it and explain .arrow_forwardHow much of a 5x buffer and how much water should be added to make 1 L of a 1x solution?arrow_forward
- A buffer contains 0.015 mol of lactic acid (pK₁ = 3.86) and 0.080 mol of sodium lactate per liter. H₂C OH Lactic acid OH Calculate the pH of the buffer. H₂C. Calculate the change in pH after adding 9.0 mL of 0.10 M HCl to 1.0 L of the buffer. O OH Sodium lactate Calculate the change in pH after adding 9.0 mL of 0.10 M HCl to 1.0 L of pure water. O Na+ buffer pH: buffer pH change: water pH change: units unitsarrow_forwardI want to make a buffer solution containing 1 M glycene pH = 10 (Na+), 1 mM ZnCl2 , 1 mM MgCl2. I have the stock solutions 1 M glycene, 1 M MgCl2, 1 M ZnCl2, and 10 M NaOH. My final volume of buffer solution needs to be 250ml. how much (in ml) of each solution should i put into my buffer?arrow_forwardYou are supplied with the following: / Jy word voorsien van die volgende: NaCl (Mr= 58,443 g /mol) 2.5M Tris-Cl, pH 8 solution /oplossing (1 Litre) EDTA (Mr= 380,2g/mol) 10% sodium dodecyl sulphate solution / natriumdodecyl sulfaat oplossing Proteïnase K solution / oplossing (50 mg dissolved / opgelos in 1 ml ddH2O) You need a digestion buffer consisting of the following: / Jy moet 'n verteringsbuffer op maak wat uit die volgende bestaan: 15m M NaCl 75 mM Tris-Cl,pH 8 16 mM EDTA,pH 8 0.8% sodium sulphate / natrium dodecyl sulfaat 0,75 mg/ml proteïnase K How will you prepare 500 ml of the digestion buffer? Show all your steps and calculations. Remember to explain exactly how you will make it up. / Hoe sal jy 500 ml van die verteringsbuffer voorberei? Wys al jou stappe en berekeninge. Onthou om te verduidelik presies hoe jy dit sal op maak.arrow_forward
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