E. coll chromosome oric AT-rich reglon 5'-GGATCCTGGGTATTAAAAAGAAGATCTATITATITAGAGATCTGTTCTAT CCTAGGACCCATAATTTITCITCTAGATAAATAAATCTCTAGACAAGATA 50 DnaA box TсTGATCTCTTАТTAGGATCGCACтсссстстGGATAАСAAGGATCGGCт ẠCACTAGAGAATAATCCTAGCGTGACGGGACACCTATIGTTCCTAGCCGA 100 51 DnaA box TTTAAGATCAACAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGAT AAATTCTAGT TGTTGGACCTTTCCTAGTAATTGACACTTACTAGCCACTA 101 150 DnaA box сстGGACCGTATАAGCTGGGATCAGAATGAGGGTTATАCACAGCTCAAAA GGACCTGGCATATICGACCCTAGTCTTACTCCCAATATGIGICGAGTIIT 200 151 ACTGAACAACGGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCT ȚGACTTGTTGCCAACAAGAAACC TATIGATGGCCAACTAGGTICGAAGGA 201 DnaA box 250 DnaA box GACAGAGTTATCCACAGTAGATCGC -3' стстстCAATAGGTGTCATCTAGCG 251 275

In the drawing shown below, the top strand (purple) is the template DNA, and the bottom shows the synthesis of the lagging strand (DNA in blue and RNA primers in red). 3' 5" ыш Left Okazaki fragment --- -11-- Middle Okazaki fragment Right Okazaki fragment 5' 3' After DNA polymerase I removes the middle primer and fills in with DNA, where is DNA ligase needed? See the arrows on both sides of the middle primer. Explain whether ligase is needed at the left arrow, at the right arrow, or both?

What part(s) of a nucleotide (namely, phosphate, sugar, and/orbase) is(are) found in the major and minor grooves of doublestrandedDNA, and what part(s) is(are) found in the DNA backbone?If a DNA-binding protein does not recognize a specificnucleotide sequence, do you expect that it binds to the majorgroove, the minor groove, or the DNA backbone? Explain.

Can you please help with 1c please  picture with 1 graph is for question 1a) picture with 4 graphs is for question 1b) 1a) E. coli DNA and binturong DNA are both 50% G-C. If you randomly shear E. coli DNA into 1000 bp fragments and put it through density gradient equilibrium centrifugation, you will find that all the DNA bands at the same place in the gradient, and if you graph the distribution of DNA fragments in the gradient you will get a single peak (see below). If you perform the same experiment with binturong DNA, you will find that a small fraction of the DNA fragments band separately in the gradient (at a different density) and give rise to a small "satellite" peak on a graph of the distribution of DNA fragments in the gradient (see below). Why do these two DNA samples give different results, when they're both 50% G-C? 1b) If you denatured the random 1000 bp fragments of binturong DNA that you produced in question 1a by heating them to 95ºC, and then cooled them down to 60ºC…

Even though the eukaryotic genome is thousands of times larger than the prokaryotic genome, DNA replication times are relatively similar. Explain how this is possible.

Using the figure below, what is molecule "A" (type a 1, 2 or 3 in the blank) nuclease ligase DNA polymerase What is the function of molecule "A"? to separate the double helix into two to piece together the Okazaki segments to copy the new DNA strand to the old strand by complementary base pairing Using the figure below, what is molecule "G" (type a 1, 2 or 3 in the blank) nuclease ligase DNA polymerase What is the function of molecule "G"? to separate the double helix into two to piece

How would I calculate sanger sequencing fragments that will produce green

All ingredients required for the synthesis of DNA were placed in a test tube.  The primer has the sequence 5’-TCGATCA-3’. First determine where the primer will bind to the DNA template given below (Do this by writing the sequence of the primer on top of the template – make sure you find a location that is complementary in sequence and that the primer is antiparallel with respect to the template) and then indicate the nucleotides that DNA polymerase would attach onto the primer as it synthesizes DNA (hint: in which direction does DNA polymerase attach nucleotides onto a primer?)  See figure 13.16 in book.   5’-GACGTAGTCTGATGCTAGCATGCTGATCGAAAGAG-3’

Which of the following pairs of base sequences could form ashort stretch of a normal double helix of DNA?(A) 5′-AGCT-3′ with 5′-TCGA-3′(B) 5′-GCGC-3′ with 5′-TATA-3′(C) 5′-ATGC-3′ with 5′-GCAT-3′(D) All of these pairs are correct

Can you please help with 1f please picture with 1 graph is for question 1a) picture with 4 graphs is for question 1b) 1a) E. coli DNA and binturong DNA are both 50% G-C. If you randomly shear E. coli DNA into 1000 bp fragments and put it through density gradient equilibrium centrifugation, you will find that all the DNA bands at the same place in the gradient, and if you graph the distribution of DNA fragments in the gradient you will get a single peak (see below). If you perform the same experiment with binturong DNA, you will find that a small fraction of the DNA fragments band separately in the gradient (at a different density) and give rise to a small "satellite" peak on a graph of the distribution of DNA fragments in the gradient (see below). Why do these two DNA samples give different results, when they're both 50% G-C? 1b) If you denatured the random 1000 bp fragments of binturong DNA that you produced in question 1a by heating them to 95ºC, and then cooled them down to 60ºC and…