E. coli strains diploid for the lac region were constructed by introducing a plasmid carrying the lac genes. The plasmid carries one copy of the lac region, and the chromosome carries the other copy. The two copies of the lac region have different genotypes, as shown in the chart below. Indicate whether the products of the lacy gene (permease) and the lacZ gene (ß-galactosidase) will be inducible, uninducible, or constitutive in each strain (assuming glucose is absent). lac region on plasmid lac region on chromosome permease B-galactosidase I-o+Z+Y- I+o+ Z-Y+ I+o+Z+Y- I+o°Z=Y+ I- oº Z+Y- I+o+ Z-Y+ Is o+ Z+Y- I+o+ Z-Y+ I+ oc Z+Y- Is o+ Z-Y+

An Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data points shown in the following graph,the conjugation was interrupted at different time points, and thepercentage of recombinants for each gene was determined bystreaking on a medium that lacked either leucine or thiamine.What information do you know based on the question and your understanding of the topic?

A sample of E.coli cells is transformed with a plasmid containing a transcriptional fusion including the promoter of an E.coli housekeeping gene and the LacZ gene. The transformed cells are plated out on selective medium containing X-gal. Which of the following best describes the likely results. a. Colonies will only be blue if lactose is also included in the growth medium. b. Colonies will only be blue if lactose and glucose are included in the growth medium c. Most of the colonies should turn blue. d. Presence of any white colonies may indicate deletion or mutation of the promoter. e. Both c. and d. are correct

A plasmid contains genes for ampicillin resistance and chloramphenicol resistance and has single sites for Hind III and Eco RI. When genes are inserted into the Eco RI site, transformants are resistant only to ampicillin. In which region is the site for EcoRI  located? Group of answer choices within the chloramphenicol gene between the ampicillin and chloramphenicol genes can't be determined within the ampicillin gene

Agrobacterium tumefaciens is a bacterium that infects a wide range of broad-leaved plants. During infection, a plasmid called Ti is transferred into the plant cells, where that plasmid DNA is integrated into the plant’s genome. Some of the genes encoded by this plasmid are expressed by host cell machinery in the plant cell. These gene products produce plant hormones which stimulate cell division in the infected cells, causing the development of a plant tumor which provides an environment for that bacterium to grow.  You are studying a novel isolate of A. tumefaciens and want to determine which of the genes on the Ti plasmid are expressed in the bacterium, and which are expressed in the plant. You start by sequencing the Ti plasmid. a)  Considering the DNA sequence of Ti, describe two specific genetic features that would help you to differentiate whether a given gene is likely expressed in the plant or bacterium. b)  You collect plant tumor tissue, containing both bacterial and plant…

The occurrence of multiple transposons within the genome oforganisms has been suggested as a possible cause of chromosomalrearrangements such as deletions, translocations, and inversions.How could the occurrence of transposons promote these types ofstructural rearrangements?

A number of mutations affect the expression of the lac operon in E. coli. The genotypes of several E. coli strains are shown below. ("+" indicates a wild-type gene with normal function and "-" indicates a loss-of-function allele.) Please predict which of the following strains would have the lowest beta-galactosidase enzyme activity, when grown in the lactose medium. Orpt o* z* r* Orpt ot z* Y OrptoztY Orrotzr OrPotz*Y

We are utilizing BL21 DE3 bacterial cells for the expression of the ADA protein via autoinduction. Create a schematic/figure showing the biological mechanism for expressing our desired protein in this cell line. We use the DE3 lysogen for expressing T7 polymerase and our plasmid has kanamycin resistance (not ampicillin).Create a schematic of this expression.

A number of mutations affect the expression of the lac operon in E. coli. The genotypes of several E. coli strains are shown below. ("+" indicates a wild-type gene with normal function and "-" indicates a loss-of-function allele.) Please predict which of the following strains would have the lowest beta-galactosidase enzyme activity, when grown in the lactose medium. OF POZY Ort Ptot Z¹ Yt Ort p²o+z¹Y+ Orpt ot zty

A transduction experiment was carried out to map the his and leu genes, which are involved in amino acid biosynthesis, of E. coli using a his+ leu+ donor and a his- leu- recipient. his+ leu- transductants will grow on agar media with: OA lacking histidine, containing leucine O B. containing histidine, containing leucine OC containing histidine, lacking leucine O D.a and b are correct O E. a, b and c are correct