Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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1) You wish to make a restriction map of a 17.0 kb linear fragment. You digest the fragment with Sbf1, Pst1, and a mixture of Sbf1 and Pst1. From
these results, you obtain these fragments following agarose gel electrophoresis of the three samples:
Sbf1: 7kb and 10kb
Pstl: 3kb, 6kb and 8kb
Sbf1 and Pstl: 1kb, 2kb, 6kb and 8kb
Question: In which of the Pstl fragments is the Sbfl site located?
8kb
O 6kb
3kb
O None of the above
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Transcribed Image Text:1) You wish to make a restriction map of a 17.0 kb linear fragment. You digest the fragment with Sbf1, Pst1, and a mixture of Sbf1 and Pst1. From these results, you obtain these fragments following agarose gel electrophoresis of the three samples: Sbf1: 7kb and 10kb Pstl: 3kb, 6kb and 8kb Sbf1 and Pstl: 1kb, 2kb, 6kb and 8kb Question: In which of the Pstl fragments is the Sbfl site located? 8kb O 6kb 3kb O None of the above
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