Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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Unlike eukaryotic organisms, bacteria can recombine their genome with foreign DNA.
What are the methods bacteria use to recombine their DNA?
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- How many chromosomes are included in a typical bacterial genome?arrow_forwardYou have ligated a foreign DNA fragment into the EcoRI site in multiple cloning site of pUCI18. You then transform how E.coli with the ligation mixture. Some of the bacterial colonies growing on the nutrient agar plate that contains ampicillin and Xgal are white and some are blue. Bacterial colonies that are white a) contain recombinant plasmid DNA b) have hydrolyzed ampicillin to form a colored compound c) are untransformed bacteria d) contain non recombinant plasmid DNAarrow_forwardAn 9 kb circular plasmid is cut with the EcoRI restriction enzyme, and the reaction products are run on a DNA gel and stained with ethidium bromide. Bands of 1, 3 and 5 kb are seen. How many EcoRI sites are in the plasmid? Choose the one answer that is most correct. a) At least 2 b) At least 3 c) At least 1 d) None e) At least 4 f) At least 5arrow_forward
- What are DNA ligases? Howdo these enzymes participatein the recombinant DNAtechnology?arrow_forwardWhy do we need a plasmid to transform a bacteria? What are the essential components of a plasmid and explain their functions. What is the difference between a Plasmid and a Vector?arrow_forwardIf you use the pUC18 vector to clone in the MCS region, predict the following: a) Do bacteria that are blue in color have a cloned insert? b)Do bacteria that are white in color have a cloned insert? c) If you were to grow these cells on Chloramphenicol (an antibiotic), would the bacteria with the pUC plasmid grow? Why or Why not?arrow_forward
- Why do we use an Electron Scanning Microscope to see bacteriophages over other microscopes?arrow_forward"Hybridization of a single-stranded DNA molecule attached to a fluorophore with a preparation of metaphase chromosomes that have been partially denatured" is a description of which laboratory method?arrow_forwarda bacteriophage has been isolated from the growth chambers that has evolved the capability to the methylate its own DNA when it is replicated inside the host bacterium. why is this a problem?arrow_forward
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