You digest a piece of DNA that is 98 000 base pairs long, with a restriction endonuclease having a recognition site four base pairs long. Approximately how many fragments would you expect to be produced? Question 18 options: 512 662 196 40 384

Q: A microbiologist discovers a new type II restriction endonuclease. When DNA is digested by this…

A: Restriction enzymes are also known as molecular scissors because they cut the DNA at specific, short…

Q: A piece of linear DNA is 700000 base pairs in length and is digested with a restriction enzyme that…

A: Linear DNA is found in prokaryotes, which is linear in shape with two free ends. Restriction enzymes…

Q: A researcher is interested in using the in vitro technique of bisulfite conversion to confirm the…

A: DNA methylation is a chemical change that frequently takes place in the setting of CpG dinucleotides…

Q: The restriction enzyme Alu I cleaves at the sequence 5’-AGCT- 3' , and Not I cleaves at 5'…

A: Restriction enzymes or restriction endonucleases are endonuclease enzymes capable of recognizing a…

Q: The red line indicates where a radioactively labeled probe hybridizes. Three samples of the genomic…

A: P                      B                         PB…

Q: Question 19. You need to prepare sequencing libraries of two different DNA fragment sizes. One…

A: A sequencing library is a collection of DNA fragments that have been prepared for high-throughput…

Q: In a Sanger DNA sequencing reaction (dideoxy method) you use the primer 5′-GCATATA-3′ to sequence a…

A: Sequencing of DNA refers to the biochemical methods for determining the order of the nucleotide…

Q: Suppose that you would like to amplify the DNA sequence below by a PCR reaction. Design 8 nucleotide…

A: Primer is a shory stretch of nucleotides which binds to 3' end of template DNA. It provides free OH…

Q: You are trying to clone a gene, You have successfully isolated it from the genomic DNA of an…

A: The term DNA cloning is associated with a process that plays an important role in making copies of a…

Q: In making recombinant DNA molecules that combine restriction fragments from different organisms,…

A: Restriction enzymes are the endonucleases that cleave the DNA.

Q: A virus with a circular double-stranded DNA chromosome contains approximately 10,000 bp. You want to…

A: To construct a map of the viral chromosome based on the cleavage sites of the restriction…

Q: You have a DNA sequence of 1800 bp. To characterize it, you decide to make a restriction map.…

A: Restriction mapping is a process that helps to determine the location of the restriction site. The…

Q: When half of the DNA in the sample is double-stranded, that point is termed the half- reaction time…

A: Cot values give the renaturation index of the DNA strands. Cot 1/2 value defines that 50%…

Q: When circular DNA is sequenced, the nucleotide base pairs are numbered starting from a fixed…

A: It is related to molecular biology in which we use restriction enzymes an important tool for

Q: A new restriction enzyme is discovered. It recognizes a 6 base pair pallindromic sequence. The first…

A: BASIC INFORMATION  RESTRICTION ENZYME  they are also known as molecular scissors it was in the year…

Q: gment shows where a number c between those cut sites in base Bem het Bem H 700 800 1000 1100

A: A restriction enzyme, also known as a restriction endonuclease or restrictase, is an enzyme that…

Q: DNA ligase

A: The correct option is Option 3. DNA ligase

Q: Using a diagram, and 3-5 sentences, explain the relationship between cell immortality and telomerase…

A: Each time a cell divides, the telomeres get shorter. Telomeres are a region of repetitive nucleotide…

Q: Choose the combination of answers that most accurately completes the statement.Which of the…

A: Restriction endonuclease or restriction enzyme is a type of enzyme used in cloning studies. It is…

Q: The restriction endonuclease NotI recognizes the octanucleotide sequence GCGGCCGC. Calculate the…

A: Restriction endonucleases are enzymes that cut DNA sequences at a specific site known as a…

Q: 5. The A DNA used for digestion was provided at a concentration of 2ug/ul. How much DNA (in…

A: DNA Should be free of contaminants such as phenol, chloroform, alcohol, EDTA, detergents or…

Q: The genomic DNA of an organism has a base composition of 60% C-G base pairs and 40% A-T base pairs.…

A: DNA stands for deoxyribonucleic acid. It is a genetic material. It is present in the nucleus and…

Q: Given the Following DNA template, TAC   CGC   TCC   GCC  GTC   GAC   AAT   ACC   ACT, write out the…

A: The DNA template is used for the synthesis of mRNA molecules. mRNA molecule is used by the…

Q: If you didn't know the sequence of the segment of DNA you want to amplify, how would you perform the…

A: Polymerase chain reaction is defined as the type of laboratory technique where it is used to amplify…

Q: Match each statement with the best possible single answer from the list below. Terms may be used in…

A:

Q: When linear DNA is sequenced, the nucleotide base pairs are numbered from the start to finish. a DNA…

A: DNA is the genetic material present in all living cells. It carries information from one cell to…

Q: Do eukaryotic cells have restriction endonucleases? Justify your answer.

A: Restriction endonucleases are the enzymes that is used to split DNA at the particular region. They…

Q: An RNA-dependent DNA polymerase that carries the RNA template with it to synthesize repeats at the…

A: Replication is the process of duplication of the DNA. Replication is initiated by recognizing the…

Q: Use the gel to answer the following questions. You will be constructing a map of the plasmid,…

A: Plasmids are circular double-stranded DNA elements. These are found in prokaryotes. The plasmids…

Q: Suppose that you would like to amplify the DNA sequence below by a PCR reaction. Design 8 nucleotide…

A: A primer is defined as the short single-stranded DNA sequence, required in the polymerase chain…

Q: X Review Chargaff's conclusions (see slide) to answer this question. DNA extracted from bacteria was…

A: The values of guanine and adenine are equivalent to quantities of cytosine and thymine.

Q: Which DNA repair systems you think might be capable of repairing a situation where T is in one…

A: DNA repair is a process by which a cell identifies and corrects damage to the DNA molecules. Most…

Q: between the two restriction sites, how would the banding pattern on the gel differ from the one you…

A: Restriction sites are the special palindromic sequences present in DNA. These sites are the location…

Q: he sequences below indicated the 6bp recognition site for the restriction enzyme EcoRI. The lines…

A: EcoRI is a restriction endonuclease that is isolated from E. coli and is used in the gene cloning…

Q: You have two tubes, each with a pair of DNA fragments inside them. Tube #1 has fragments that are…

A: Electrophoresis  Electrophoresis is used in biological laboratory to separate different size of DNA,…

Q: Which of the following is required in running a PCR reaction? Promoter Primase DNTPS DNA polymerase…

A: PCR is polymerase chain reaction , which is a biotechnology tool used in DNA amplification.

Q: You used agarose gel electrophoresis to separate DNA fragments of different size and the experiment…

A: Agarose gel electrophoresis is a method to separate, identify and purify the DNA molecules.…