You digest a piece of DNA that is 98 000 base pairs long, with a restriction endonuclease having a recognition site four base pairs long. Approximately how many fragments would you expect to be produced? Question 18 options: 512 662 196 40 384

Q: You are studying a large eukaryotic gene that is 439,515 base pairs long. You find the polypeptide…

A: As proteins are polymers of amino acids, the chemical composition of the amino acid side chains and…

Q: need to understand how to know which is 5' overhand and which is 3' overhang

A: EcoRI is a restriction enzyme-producing sticky ends. It identifies the specific sequence GAATTC on…

Q: A researcher is interested in using the in vitro technique of bisulfite conversion to confirm the…

A: DNA methylation is a chemical change that frequently takes place in the setting of CpG dinucleotides…

Q: The red line indicates where a radioactively labeled probe hybridizes. Three samples of the genomic…

A: P                      B                         PB…

Q: Question 19. You need to prepare sequencing libraries of two different DNA fragment sizes. One…

A: A sequencing library is a collection of DNA fragments that have been prepared for high-throughput…

Q: A virus with a circular double-stranded DNA chromosome contains approximately 10,000 bp. You want to…

A: To construct a map of the viral chromosome based on the cleavage sites of the restriction…

Q: Choose the combination of answers that most accurately completes the statement.Why must the lagging…

A: DNA replication is the process through which DNA synthesizes the complementary strands with the help…

Q: Specifically, why is the primer needed in order for DNA ploymerase to start synthesis? A ) primers…

A: Always replication, Transcription starts from 5' to 3'. Addition of nucleotide takes place at 3'-OH.

Q: You want to clone the Human Gene A into a plasmid for producing the Protein A in bacteria. GeneA…

A: Introduction :- A plasmid is a small, circular, double-stranded DNA molecule that is separate from…

Q: How would you solve this question? I am confused

A: The objective of this question is to estimate the size of an unknown DNA fragment using a standard…

Q: Which primer would be appropriate to amplify the top strand of the DNA molecule shown? 5'…

A: Polymerase chain reaction is a method widely being used to make multiple copies of the same DNA…

Q: A new restriction enzyme is discovered. It recognizes a 6 base pair pallindromic sequence. The first…

A: BASIC INFORMATION  RESTRICTION ENZYME  they are also known as molecular scissors it was in the year…

Q: DNA ligase

A: The correct option is Option 3. DNA ligase

Q: Choose the combination of answers that most accurately completes the statement.Which of the…

A: Restriction endonuclease or restriction enzyme is a type of enzyme used in cloning studies. It is…

Q: The restriction endonuclease NotI recognizes the octanucleotide sequence GCGGCCGC. Calculate the…

A: Restriction endonucleases are enzymes that cut DNA sequences at a specific site known as a…

Q: One way scientists determine the abundance of different DNA molecules in a sample is by measuring…

A: Polymerase chain reactionPCR is a rapid and versatile in vitro method for amplifying defined target…

Q: 5. The A DNA used for digestion was provided at a concentration of 2ug/ul. How much DNA (in…

A: DNA Should be free of contaminants such as phenol, chloroform, alcohol, EDTA, detergents or…

Q: If you have got the following DNA template molecules, which one of them will require more energy to…

A: Melting temperature is the temperature at which a double-stranded molecule of DNA is broken down…

Q: Assume that a cycle of the polymerase chain reaction takes 20 minutes. How many copies of a single…

A: The objective of the question is to calculate the number of copies of a single DNA fragment after…

Q: If you didn't know the sequence of the segment of DNA you want to amplify, how would you perform the…

A: Polymerase chain reaction is defined as the type of laboratory technique where it is used to amplify…

Q: Your mutagenized sample showed 523 CFU after plating 100uL of media on the 10^3 dilution plate.  The…

A: CFU/ml = CFU/(Dilution × Volume plated)  CFU stands for colony forming units.

Q: Match each statement with the best possible single answer from the list below. Terms may be used in…

A:

Q: Match each statement with the best possible single answer from the list below. Terms may be used in…

A: Addition of TTGGGG repeats Correct match for this statement are - Telomerase.   Explanation…

Q: Of the three key building blocks of DNA, which type(s) of building block is/are structurally…

A: DNA and RNA are the genetic material found in living organisms. RNA is mostly observed in viruses.…

Q: Below is a generalized figure of replicating DNA. Describe what is occurring for each figure.

A: DNA replication is the process of new DNA synthesis from the old DNA molecule that occurs within the…

Q: Suppose that you would like to amplify the DNA sequence below by a PCR reaction. Design 8 nucleotide…

A: A primer is defined as the short single-stranded DNA sequence, required in the polymerase chain…

Q: The human genome has approximately 30,000 genes, but human cells can produce over 100,000 different…

A: The human genome consists of complete set of sequences of nucleic acid which are encoded as DNA.…

Q: Which DNA repair systems you think might be capable of repairing a situation where T is in one…

A: DNA repair is a process by which a cell identifies and corrects damage to the DNA molecules. Most…

Q: Determine which primer pair is the best choice. • Explain why the other primers are not good…

A: A brief DNA or RNA sequence serves as the template and controls the production of a complementary…

Q: There are 2 parts to this question: The following DNA strand (below) is about to undergo DNA…

A: The cellular functions are regulated/controlled by the DNA present within the nucleus of the cell.…

Q: Give typing answer with explanation and conclusion to all parts Maxim-Gilbert and Sanger Sequencing…

A: DNA sequencing is a technique that involves reading the order of the arrangement of DNA bases. Maxam…

Q: Dideoxy sequencing suitable for determining the sequence of one DNA molecule high error rate per…

A: Dideoxy sequencing, sometimes referred to as Sanger sequencing, is an established technique for DNA…

Q: Which of the following is required in running a PCR reaction? Promoter Primase DNTPS DNA polymerase…

A: PCR is polymerase chain reaction , which is a biotechnology tool used in DNA amplification.

Q: You used agarose gel electrophoresis to separate DNA fragments of different size and the experiment…

A: Agarose gel electrophoresis is a method to separate, identify and purify the DNA molecules.…

Q: A hypothetical map of a 10,000 bp (base pair) segment of a human chromosome is shown (1 kbp= 1,000…

A: In this experiment, DNA from two different individuals, Individual 1 and Individual 2, is extracted…

Q: The polymerase chain reaction (PCR) is used by se quantity of DNA is very small, mixed, or contamin…

A: The PCR reaction is the reaction that is used by scientists and researchers majorly for…