You digest a piece of DNA that is 98 000 base pairs long, with a restriction endonuclease having a recognition site four base pairs long. Approximately how many fragments would you expect to be produced? Question 18 options: 512 662 196 40 384

A 2.0kb bacterial plasmid ‘BS1030’ is digested with the restriction endonuclease Sau3A; the plasmid map is depicted in the diagram below and the Sau3A (S) restriction sites are indicated. Which of the following DNA fragments do you expect to see on an agarose gel when you run Sau3A-digested plasmid ‘BS1030’ DNA?   a. 250 bp, 450 bp, 550 bp, 1.1 kb, 1.5 kb and 2.0 kb   b. 2.0kb   c. 250 bp, 400 bp, 450 bp, 500 bp and 550 bp   d. 100 bp, 200 bp, 250 bp, 400 bp, 500 bp and 550 bp

A 10 kb DNA fragment digested with the restriction endonuclease EcoRI yields fragments of 4 kb and 6 kb.  When the 10 kb fragment is digested with BamHI, three fragments of 1, 3.5 and 5.5 kb are generated.  Digestion with both enzymes yields four fragments of 0.5, 1, 3 and 5.5 kb.  Draw the restriction map for the 10 kb fragment based on the data.  Label the cut sites for the two enzymes, and the lengths between the cut sites.

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The sequence below shows 140bp of a double-stranded DNA sequence coding for histone H2A. What two primers could be used to amplify the whole 140bp sequence in a PCR reaction? 1                                                                   70ATGTCTGGACGTGGCAAGCAGGGAGGCAAAGCCCGCGCTAAGGCCAAGACTCGCTCTTCTAGGGCCGGTCTACAGACCTGCACCGTTCGTCCCTCCGTTTCGGGCGCGATTCCGGTTCTGAGCGAGAAGATCCCGGCCAG 71                                                                 140 TCCAGTTCCCCGTGGGCCGAGTGCACCGCCTGCTCCGCAAAGGCAACTATGCCGAGCGGGTCGGGGCCGGAGGTCAAGGGGCACCCGGCTCACGTGGCGGACGAGGCGTTTCCGTTGATACGGCTCGCCCAGCCCCGGCC   a. TACAGACCTGCACCGTTC and GGCCGGGGCTGGGCGAGC   b. ATGTCTGGACGTGGCAAG and CCGGCCCCGACCCGCTCG   c. ATGTCTGGACGTGGCAAG and GCTCGCCCAGCCCCGGCC   d. TACAGACCTGCACCGTTC and CGAGCGGGTCGGGGCCGG

In the table below, there are four versions of gene A, one of which is normal, and the other three which contain mutations that make the gene product nonfunctional. Focus on the shaded region of the sequence. Use the genetic code table to answer the question. How would you describe Mutation #2? Partial DNA sequence for gene A ("..." indicates many nucleotides of sequence not shown) 5' ... ATG GTG AGC AAG GAG GAG CTG TTC ACC TGT AAA TAG ... Normal Mutation #1 5' ... ATG GTG AGC AAG GAG AAG CTG TTC ACC TGT AAA TAG ... Mutation #2 5' ... ATG GTG AGC AAG TAG GAG CTG TTC ACC TGT AAA TAG ... Mutation #3 5' ... ATG GTG AGC AAG GAG CTG TTC ACC TGT AAA TAG ... Silent mutation Nonsense mutation Frameshift mutations Missense mútation

Use the diagram to answer the following three questions. 5' TTAGGGITAGGGTTAG 3' ||||| 3' AATCCC 5' -Telomerase ČAAUCCCAAUC RNA of telomerase- 3' 5' Write the next six bases that would be added by telomerase using the format 5'NNNNNN3' What does the "RT" stand for in the protein known as TERT (two words)? In order to add more than six bases what must the telomerase do first?

E 1 kb 1 kb DAK 6 kb Gene X E1 kb B 5 kb. Gene Z E₁kb 1 kb The figure above shows a linear chronosome containing Gene X and Gene Z and the location of sites for the restriction enzymes EcoRI (E) and BamHI (B). Refer to this map as you answer the question below. If you digest this DNA with EcoRI, then perform agarose gel electrophoresis, how many bands, and what sizes, would you expect to see? a) Two bands on the gel corresponding to the sizes 2 kb and 6 kb b) Three bands on the gel corresponding to the sizes 1 kb, 6 kb and 8 kb. c) Three bands on the gel corresponding to the sizes 1 kb, 5 kb and 6 kb d) Four bands on the gel corresponding to the sizes 1 kb, 2 kb, 5 kb and 6 kb e) Four bands on the gel corresponding to the sizes 2 kb, 3 kb, 4 kb and 8 kb