Give typing answer with explanation and conclusion to all parts Maxim-Gilbert and Sanger Sequencing are two different methods used to sequence DNA. Describe the general techniques of Maxim-Gilbert and Sanger DNA Sequencing. List the advantages and disadvantages of each.

Q: what is the whole-genome shotgun sequencing? Also briefly explain its strategy to assemble the…

A: Whole - genome shotgun sequencing is a single step sequencing method, in which the genome is broken…

Q: List and describe three important processes are affected by DNA supercoilin

A: DNA supercoiling is reffered to as under or "overwinding' of DNA strands. It is a crucial process in…

Q: How is the color of the spot coverted into useful data if data is collected from a Vicrochip DNA…

A: DNA microarray is a chip-based technique where a collection of microscopic DNA spots attached to a…

Q: Why is genome database and annotation relevant to Genome sequencing?   Are there any examples?

A: Genome: the set of genes. Genome database: it is the collection of all information on the human…

Q: Why is Sanger sequencing sometimes referred to as "dye-terminator" sequencing?

A: Frederick Sanger and his colleagues created Sanger Sequencing, often known as 'chain termination…

Q: Describe the components vital for DNA sequencing with the Sanger method

A: DNA sequencing: The process of identifying the correct order of the four nitrogen bases (adenine,…

Q: From your knowledge about DNA microarray, answer the following: Mention the name and the color of…

A: DNA microarray is basically a collection of microscopic DNA spots that are attached to the solid…

Q: Protocols in isolating DNA often involve the use of two kinds of ethanol, 100% ethanol and  70%…

A: 70% ethanol is used in washing DNA furing DNA extraction. 100% ethanol is used in the final step. In…

Q: Propose a method for isolating a DNA fragment that is adjacent in the genome to a previously…

A: Deoxyribonucleic acid is a particle made out of two polynucleotide chains that loop around one…

Q: Aside from Sanger Sequencing, what are the other DNA sequencing methods that are available today?…

A: Sanger sequencing is a DNA sequencing technology that uses electrophoresis and is based on DNA…

Q: Read up on the latest developments in DNA sequencing, and present a summary of any one such…

A: DNA (deoxyribonucleic acid) sequencing is the technique of identifying or determining the order of…

Q: The exponential nature of PCR allows spectacular increases in the abun- dance of a DNA sequence…

A: PCR (polymerase chain reaction is a technique through which a DNA segment is copied to a million…

Q: Let's say that an experiment resulted in the production of several unattached kinetochores. What…

A: It includes cell division, replication and growth. All these processes should be carried out in a…

Q: List all the components and explain their purpose in the reaction mixture used for the dideoxy DNA…

A: The method of evaluating the sequence of nucleotides (As, Ts, Cs, and Gs) in a portion of DNA is DNA…

Q: In isolating eukaryotic DNA, it is essential that the DNA is not sheared, and that high molecular…

A: DNA extraction refers to separating DNA from protein, membrane, and other biological components in…

Q: Consider a genome whose length is 1000 bp. "Shotgun" sequencing techniques are applied to the…

A: Shotgun sequencing is an important tool in modern genomics that allows researchers to interpret the…

Q: tail the process of Sanger sequencing. Include a diagram

A: Sequencing of genetic material involves visualizing the number and arrangement of base pairs. It is…

Q: Why a multiple sequence alignment is needed for researchers? What inferences can be derived from…

A: When three or more biological sequences are aligned and studied together, it is referred to as…

Q: What is expected theoretical number of copies of DNA molecules after 28 cycles in a PCR experiment?…

A: INTRODUCTION: DNA : Its fullform is Deoxyribo nucleic acid and It has a double stranded helix…

Q: Use the data in the table below and the gel picture to create a graph (using the graphic paper…

A: The given data states fragments created by BamHI, EcoRI , HindIII as represented in Agarose Gel…

Q: (1) What is the major difference between the conventional PCR and real time PCR (qPCR) in terms of…

A: Question. What is the major difference between the conventional PCR and real time PCR (qPCR) in…

Q: List and Describe the algorithm of BLOSUM substitution matrix computation.

A: The BLOSUM matrix is another amino acid substitution matrix, first determined by Henikoff. For its…

Q: Explain why DNA ladders are usually included during gel electrophoresis.

A: BASIC INFORMATION  GEL ELECTROPHORESIS  It is a process in which the DNA fragments are separated…

Q: Describe the possible outcome of a PCR experiment in which (a) one of the primers is inadvertently…

A: The polymerase Chain reaction is molecular biology in vitro technique to make multiple copies of a…

Q: Align two sequences: horizontal - GGAATGG, vertical – ATG, m = 1, mm = 0, g = -1. Use the table…

A: Global Alignment: It is a bioinformatics algorithm used to align protein or nucleotide sequences. A…

Q: Your colleague made the table below comparing DNA Polymerase to RNA Polymerases. Which row is…

A: Transcription is the molecular process which involves the DNA sequences that are transcribed as…

Q: Give a step by step method and diagram of how reversible terminator sequencing works.

A: The reversible terminator sequencing or Illumina dye sequencing is a technique used to determine the…

Q: enzymes ECORI and HINDIII are called "compatible" enzymes. DNA cut with these enzymes can be ligated…

A: Restriction Endonucleases Restriction endonucleases or restriction enzymes are the enzymes that can…

Q: Discuss the principles , uses, advantages and disadvantages of illumina sequencing method

A: DNA copies obtained from PCR is subjected for gene sequencing through different gene sequencing…

Q: Discuss at least three methods of DNA extraction and summarize each methods using a schematic…

A: DNA extraction is a technique to separate DNA from cell membranes, proteins, and other biological…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.