PART V: ESTIMATING THE SIZE OF THE UNKNOWN DNA FRAGMENT The standard used in this lab is Lambda DNA digested with the restriction enzyme Hind III, which created the following fragments: 0.12kb, 0.56kb, 2.02kb, 2.32kb, 4.36kb, 6.57kb, 9.4kb, and 23.13kb (Figure 11.4). 23130- 9416- 1-36557- 24361- 32322- 2027 564- 56 125- Figure 11.4. Marker DNA stained with ethidium bromide Exercise 1: Using Figure 11.5 on the next page representing an agarose gel, complete the following. You must estimate the size of the unknown DNA fragment (fragment X) using the DNA standard. Use the provided semi-log graph paper to plot the log of the molecular weight of each of the standard bands (shown in Figure 11.4) vs. the distance travelled from the well based on measurements made from Figure 11.5 (do not calculate log of molecular weight, just plot molecular weight-the calculation is incorporated. into the semi log scale of graph paper). Draw the line of best fit between the points. From this plot, you should be able to determine the molecular size of the unknown DNA.

Can Chris be Frank’s father?  Why or why not?

QUESTION 5 It is best to receive an organ transplant from a donor who O looks like you O rarely consumes alcohol O does not have any allergies O best matches your self proteins

I need help with my questions. & are the ones I answer correct ?

I can only have one answer so would it be the first one or the second? If it started with the 5 prime end

Am stands for

9. A male patient (83-kg IBW) is intubated and ventilated with PC-CMV, f = 12/min, set PIP = 28 cm H2O, resulting in a VT of 430 mL. The ABG results on this setting are: pH 7.35, PaCO2 45 mm Hg, and HCO3 − 23 mEq/L. Forty-eight hours later on the same settings, the ABG results are: pH 7.54, PaCO2 27 mm Hg, and HCO3− 21 mEq/L with an exhaled VT of 800 mL. The most appropriate action at thistime is which of the following?a. Decrease PIP to 25 cm H2O.b. Decrease PIP to 19 cm H2O.c. Decrease f to 10/min.d. Decrease f to 8/min.

Is that the only correct option ?

Can Alex be Frank’s father?  Why or why not?

In my homework this solution was wrong. Could there be another option for this question?