PART V: ESTIMATING THE SIZE OF THE UNKNOWN DNA FRAGMENT The standard used in this lab is Lambda DNA digested with the restriction enzyme Hind III, which created the following fragments: 0.12kb, 0.56kb, 2.02kb, 2.32kb, 4.36kb, 6.57kb, 9.4kb, and 23.13kb (Figure 11.4). 23130- 9416- 1-36557- 24361- 32322- 2027 564- 56 125- Figure 11.4. Marker DNA stained with ethidium bromide Exercise 1: Using Figure 11.5 on the next page representing an agarose gel, complete the following. You must estimate the size of the unknown DNA fragment (fragment X) using the DNA standard. Use the provided semi-log graph paper to plot the log of the molecular weight of each of the standard bands (shown in Figure 11.4) vs. the distance travelled from the well based on measurements made from Figure 11.5 (do not calculate log of molecular weight, just plot molecular weight-the calculation is incorporated. into the semi log scale of graph paper). Draw the line of best fit between the points. From this plot, you should be able to determine the molecular size of the unknown DNA.

PART V: ESTIMATING THE SIZE OF THE UNKNOWN DNA FRAGMENT The standard used in this lab is Lambda DNA digested with the restriction enzyme Hind III, which created the following fragments: 0.12kb, 0.56kb, 2.02kb, 2.32kb, 4.36kb, 6.57kb, 9.4kb, and 23.13kb (Figure 11.4). 23130- 9416- 1-36557- 24361- 32322- 2027 564- 56 125- Figure 11.4. Marker DNA stained with ethidium bromide Exercise 1: Using Figure 11.5 on the next page representing an agarose gel, complete the following. You must estimate the size of the unknown DNA fragment (fragment X) using the DNA standard. Use the provided semi-log graph paper to plot the log of the molecular weight of each of the standard bands (shown in Figure 11.4) vs. the distance travelled from the well based on measurements made from Figure 11.5 (do not calculate log of molecular weight, just plot molecular weight-the calculation is incorporated. into the semi log scale of graph paper). Draw the line of best fit between the points. From this plot, you should be able to determine the molecular size of the unknown DNA.

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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