PART V: ESTIMATING THE SIZE OF THE UNKNOWN DNA FRAGMENT The standard used in this lab is Lambda DNA digested with the restriction enzyme Hind III, which created the following fragments: 0.12kb, 0.56kb, 2.02kb, 2.32kb, 4.36kb, 6.57kb, 9.4kb, and 23.13kb (Figure 11.4). 23130- 9416- 1-36557- 24361- 32322- 2027 564- 56 125- Figure 11.4. Marker DNA stained with ethidium bromide Exercise 1: Using Figure 11.5 on the next page representing an agarose gel, complete the following. You must estimate the size of the unknown DNA fragment (fragment X) using the DNA standard. Use the provided semi-log graph paper to plot the log of the molecular weight of each of the standard bands (shown in Figure 11.4) vs. the distance travelled from the well based on measurements made from Figure 11.5 (do not calculate log of molecular weight, just plot molecular weight-the calculation is incorporated. into the semi log scale of graph paper). Draw the line of best fit between the points. From this plot, you should be able to determine the molecular size of the unknown DNA.
PART V: ESTIMATING THE SIZE OF THE UNKNOWN DNA FRAGMENT The standard used in this lab is Lambda DNA digested with the restriction enzyme Hind III, which created the following fragments: 0.12kb, 0.56kb, 2.02kb, 2.32kb, 4.36kb, 6.57kb, 9.4kb, and 23.13kb (Figure 11.4). 23130- 9416- 1-36557- 24361- 32322- 2027 564- 56 125- Figure 11.4. Marker DNA stained with ethidium bromide Exercise 1: Using Figure 11.5 on the next page representing an agarose gel, complete the following. You must estimate the size of the unknown DNA fragment (fragment X) using the DNA standard. Use the provided semi-log graph paper to plot the log of the molecular weight of each of the standard bands (shown in Figure 11.4) vs. the distance travelled from the well based on measurements made from Figure 11.5 (do not calculate log of molecular weight, just plot molecular weight-the calculation is incorporated. into the semi log scale of graph paper). Draw the line of best fit between the points. From this plot, you should be able to determine the molecular size of the unknown DNA.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:PART V: ESTIMATING THE SIZE OF THE UNKNOWN DNA FRAGMENT
The standard used in this lab is Lambda DNA digested with the restriction enzyme Hind III, which
created the following fragments: 0.12kb, 0.56kb, 2.02kb, 2.32kb, 4.36kb, 6.57kb, 9.4kb, and 23.13kb
(Figure 11.4).
23130-
9416-
1-36557-
24361-
32322-
2027
564-
56
125-
Figure 11.4. Marker DNA stained with ethidium bromide
Exercise 1: Using Figure 11.5 on the next page representing an agarose gel, complete the following.
You must estimate the size of the unknown DNA fragment (fragment X) using the DNA standard. Use
the provided semi-log graph paper to plot the log of the molecular weight of each of the standard bands
(shown in Figure 11.4) vs. the distance travelled from the well based on measurements made from Figure
11.5 (do not calculate log of molecular weight, just plot molecular weight-the calculation is incorporated.
into the semi log scale of graph paper). Draw the line of best fit between the points. From this plot, you
should be able to determine the molecular size of the unknown DNA.
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