Yes or no? reverse genetics is RNA interference example. cellular differentiation potency in multipotent is greater than pluripotent stem cell. does digoxigenin UTO use to make dsrna and perform rna interference?
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Yes or no?
reverse genetics is RNA interference example.
cellular differentiation potency in multipotent is greater than pluripotent stem cell.
does digoxigenin UTO use to make dsrna and perform rna interference?
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- 5 5 S 6 5 5 5 6 U 6 U 6 5:14 PM | 0.2KB/s HHHHH R R U RUUR ARU AP AP R U U R R AP R R R AP MOLECULAR...GENETICS. Describe gene regulation at transcription level. Explain the role of antsense RNA in control mechanism. Describe translational control mechanisms. Describe common DNA damages. Distinguish excision and mismatch repair. Describe the role of recA protein in recombination repair Elaborate on SOS repair mechanism. Define thymine dimer. How are they formed and repaired? Describe the molecular basis of mutation. 11 Leu+ Met+ Arg+ Write a detailed note on spontaneous mutation. Explain about mutant detection methods. Define reverse mutation. Describe the mechanism underlying Intragenic and intergenic suppressor mutations Describe the transposition mechanisms. 13 Vo LTE UNIT IV Time (Min) Describe the process of generalised transformation occurring in bacterial chromosome and plasmid. Elaborate on molecular mechanism and significance of transformation 22 Describe the process of…Which of the following is least related to the other items? Group of answer choices inducer repressor operator enhancers 2. A scientist observing a cell during gene expression would be able to easily distinguish it as a prokaryotic cell by which of the following observations? Group of answer choices as soon as the DNA introns are removed from the template after the 5' caps are converted to mRNA after a transcription initiation complex has been formed during transcription once the pre-mRNA has been converted to mRNAWhy are bacterial polyribosomes formed co-transcriptionally? Check all that apply. Bacterial ribosomal subunits cannot enter the nucleus. Bacterial ribosomal subunits can enter the nucleus. Bacterial ribosomal subunits cannot enter the nucleoid. Bacterial ribosomal subunits can enter the nucleoid. Bacterial ribosomal subunits cannot bind mRNAs before transcription termination. Bacterial ribosomal subunits can bind mRNAs before transcription termination.
- YFP-SC35 encodes a splicing factor. You would expect this molecule to be localized in what compartment of the cell? nucleoli nucleus cytoplasm mitochondriaFirst, I bind to an unstructured region of RNA and moves toward its 3’ end. Then RNA polymerase encounters a terminator sequence and I am able to catch up. Finally, using helicase activity, I am able to unbind the DNA-RNA hybrid and transcription stops. Who am I? What process do I work in? Group of answer choices Rho, in rho- dependent terminator in eukaryotes Rat1, in rho-independent terminators in prokaryotes Rho, in rho- dependent terminators in bacteria The correct answer is not available Rat1, in termination in eukaryotesWhich of the following is a likely outcome of Rifampin treatment? Transcriptional elongation is blocked Peptidyltransferase activity is inhibited at the ribosome The peptidoglycan cell wall is degraded by peptidoglycan hydrolases Double stranded DNA breaks Pore formation in the outer membrane
- Please match the gene element with the the correct definition. control elements 5' UTR 3' UTR Promoter coding region +1 site [Choose ] stretch of DNA that contains the sequence that will encode the protein the active form of RNA polymerase region of DNA upstream of transcription start site that is recognized by RNA polymerase region of mRNA on the 5' end that goes untranslated RNA polymerase and the sigma factor regions of DNA that increase or decrease transcription rate the first nucleotide to be transcribed region of mRNA on the 3' end that goes untranslated a transcription initiation factor that helps RNA polymerase recognize the promoter the inactive form of RNA polymerase [Choose ] [Choose ]Suppose multiple mutations occur in the U1 snRNA. Which step in the mRNA Spllcing mechanism would be directly impaired? The second transesterification reaction 5' splice site/donor site recognition 3' splice site/acceptor site recognition O Branch site recognitionWhich of the following characterize RNA polymerase Il transcriptional termination in eukaryotes? Endonuclease cleavage of the RNA transcript and 5' to 3' exonuclease activity a protein known as Ratl None of the provided answers, transcriptional termination occurs in prokaryotes. Recognition of the transcriptional stop codon by a release factor. Hairpin structure formation on the newly synthesized RNA molecule which disrupts the DNA-RNA hybrid at a poly-U RNA sequence Binding of the Rho protein.
- Why might repression of a eukaryotic gene by an RNA be more efficient than repression by a protein repressor? O Protein repressors only prevent the synthesis of a single splice variant of a gene. O RNA repressors bind directly to nucleotide sequences in genes, preventing their transcription. O Protein repressors are inherently unstable, so they degrade quickly and do not completely repress genes. O Synthesis of RNA repressors is less energetically costly than that of protein repressors.Select whether each of the components are associated with eukaryotic or prokaryotic transcription. (When studying for exams, think about what the other component would be!) Prokaryote or Eukaryote?? RNA polymerase + sigma protein: TATA box: General/basal transcription factors: Rho-independent/dependent termination: [At 2:00 pm, you measured intracellular arginine at 100M. After addition of 200M of arginine to the cell, you, at 2:45 pm, measured intracellular arginine levels to be still at 100M. The most likely explanation is: Arginine bound to the active site of the repressor protein Arginine bound to the allosteric site of RNA polymerase Arginine was in excess in the cell and acted as an inducer Arginine was in excess in the cell and bound to the operator Arginine bound to the allosteric site of the repressor protein