What are the differences between In vitro and In vivo
Question:
What are the differences between Invitro and Invivo DNA replication?
Answer:
Invitro DNA replication is using the PCR technique in the test tube and Invivo DNA replication is the replication process that takes place in the living cells.
PCR synthesizes huge quantities of DNA from a very small quantity of nucleic acid as starting material.
In PCR, the methodology involves similar to the process that takes place in the living cells.
Invivo DNA synthesis in living cells depends on a well-defined complex set of enzymes and cofactors.
PCR, when compared to Invivo, is a simpler way using a smaller set of ingredients that are predetermined and reactions take place at relatively higher temperatures for annealing and denaturation of DNA strands.
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