Which among the following statements is correct?O lon-exchange chromatography is dependent on the charge and size of the target componentAffinity chromatography is dependent on the affinity of the target component to the mobile phaseBoth statements are correctO Both statements are incorrect

Introduction: Chromatography is an analytical technique for the separation of different dissolved…

Answered: Which among the following statements is…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

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Problem 1P

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Plate A = filter paper dipped into 5% solution of unknown Dye A %3D Plate B = filter paper dipped into 5% solution of unknown Dye B Both plates were incubated at 37°C. At the end of the 60 minute incubation period the diffusion rings for each dye were measured. The diffusion ring for Dye A = 40 mm. The diffusion ring for Dye B = 24 mm %3D Based on the results, which dye had the higher molecular weight? Explain your answer.
During a temperature-programmed desorption (TPD) experiment it is observed that the temperature of a particular desorption peak decreases as the initial coverage of the reacting species increases. Account for this observation making reference to the orderof the desorption process.
uring dialysis, a semi-permeable membrane with a molecular weight cutoff of 10,000 is used. Select the true statements from the list below (more than one answer could apply) Group of answer choices A monoclonal antibody with a 150 kDa MW is retained and does not diffuse across the membrane A small enzyme with 20 kDa MW diffuses readily across the membrane Buffer salts with molecular weights ranging between 100 and 500 Da diffuse across the membrane until equilibrium (equal concentration) is reached on both sides of the membrane None of the above statements are correct Please answer asap
1) If direct stains work so well, why would we ever use indirect stains? Give two reasons why you might choose to use indirect stains instead of direct stains? 2) Assume that two days (dye A and dye B) were applied to bacterial smear. The cells were stained by dye A while the slide background was stained with dye B. Which stain had an anionic chromogen? Which stain had a cationic chromogen?
The primary difference between plasma and intracellular fluid, is that Intracellular fluid has a higher Na concentration O Plasma has a higher Ca concentration O Intracellular fluid has a higher protein level OPlasma has significantly more nitrates
The early steps of rabiesvirus (CVS) infection in vitro were studied in chicken embryo cells (CER) cells. The infection was monitored by looking for specific viral inclusions using anti-rabies fluorescein isothiocynanate at 24 hours after addition of virus. Cells were incubated for various lengths of time after virus attachment with ammonium chloride and chloroquine. The results are show in Figure 2. 100 75 50 25 1 3 5 7. 24 Time (h) (1) 20 mM NHẠCI (0) 0.1 mM chloroquine Figure 2 What is the effect of ammonium chloride on the infection of CER cells? Explain why there are such effects. Fluorescence inhibition (%)
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Which among the following statements is correct? Group of answer choices Both statements are incorrect Negatively-charged proteins are eluted out first when a cation exchanger is used as the stationary phase for ion-exchange chromatography Both statements are correct Proteins that have higher affinity to the ligands are eluted out last in affinity chromatography
You are working on a team that is designing a new drug. For this drug to work, it must enter the cytoplasm of specific target cells. Which of the following would be a factor that determines whether the molecule selectively enters the target cells? O the phospholipid composition of the target cells' plasma membrane O the nonpolar, hydrophobic nature of the drug molecule the similarity of the drug molecule to other molecules that are transported into the target cells the concentration of the drug molecule that is transported in the blood Submit Request Answer
The classic demonstration that cell plasma membranes are composed ofbilayers depends on the following kinds of data:• The membrane lipids from 4.74 x 109erythrocytes will form a monolayer of area 0.89 m2when spread on a water surface.• The surface of one erythrocyte is approximately 100 μm2in area. Show that these data can be accounted for only if the erythrocyte membrane is a bilayer.
A cell stops proliferating when treated with a chemical analog to fucoxanthin. When these cells are analyzed and compared to untreated cells according to the amount of DNA they contain using flow cytometry, the graphs in the figure below are obtained. Which of the following answers would NOT cause this result?
Which of the following would you not expect to find in a human erythrocyte? O Band 4.3 Spectrin Actin Glycophorin Smooth Endoplasmic Reticulum

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Which among the following statements is correct? O lon-exchange chromatography is dependent on the charge and size of the target component Affinity chromatography is dependent on the affinity of the target component to the mobile phase Both statements are correct Both statements are incorrect