Which among the following statements is correct?O lon-exchange chromatography is dependent on the charge and size of the target componentAffinity chromatography is dependent on the affinity of the target component to the mobile phaseBoth statements are correctO Both statements are incorrect

Introduction: Chromatography is an analytical technique for the separation of different dissolved…

Answered: Which among the following statements is…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Which of the following is TRUE about immunofluorescence microscopy where we localize proteins using fluorescently tagged antibodies? Pick the best answer: We can use it to resolve 2 points that are 10 nanometers apart. The emission wavelength of the fluorophore is typically shorter than the excitation wavelength. The resolution can be further improved with increased magnification. All else being equal, a fluorophore that emits red light will give better resolution than a fluorophore that emits green light. Based on what we discussed in class, we can use the technique on live samples therefore enabling us to see the dynamic movement of cytosolic proteins in living cells. None of the above
A
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i found this past paper however i cannot find the solutions, please can you answer question 1b
If a red blood cell was placed in a 50mOSM solution, the cell would:
The early steps of rabiesvirus (CVS) infection in vitro were studied in chicken embryo cells (CER) cells. The infection was monitored by looking for specific viral inclusions using anti-rabies fluorescein isothiocynanate at 24 hours after addition of virus. Cells were incubated for various lengths of time after virus attachment with ammonium chloride and chloroquine. The results are show in Figure 2. 100 75 50 25 1 3 5 7. 24 Time (h) (1) 20 mM NHẠCI (0) 0.1 mM chloroquine Figure 2 What is the effect of ammonium chloride on the infection of CER cells? Explain why there are such effects. Fluorescence inhibition (%)
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Po ~Av Αν Radioactive Thymine (գլոսյա/siunos) 500 400- 300- 200- 100- Radioactive Uracil (counts/minute) Figure 1- Radioactive Thymine E-621 3¶ = = = = 112 0- 100 80- 60- 40- 20 15 20 Time (minutes) Figure 3-Radioactive Uracil 1 1 0- 14 ted States) IT 1 5 10 5 2 10 25 25 20 15 Time (minutes) 1 30 111111 30 V g DNA in broth 35 2- 1.5- 1- 0.5- 0 3 35 Test broth with antibiotic added Control broth with no antibiotic added 1 V 40 glucose concentration to kill the bacteria. 40 Figure 5-DNA in the Broth 2.5- 5 L AaBbCcDdEe AaBbCcDdEe AaBb AaBbC Normal No Spacing Heading 1 Heading 2 Radioactive Methionine 10 (counts/minute) 30,000 25,000- 20,000- 15,000- Figure 2- Radioactive Methionine 10,000- 5,000- 0++ 500- 400- 300- 200- 100- Figure 4- Glucose Concentration 0 25 1 15 20 Time (minutes) LL MacBook Pro 1 30 5 5 f 5 35 W 20 25 15 10 Time (minutes) 10 40 6 LI 25 15 20 Time (minutes) 30 30 35 35 40 40 Foc
The growth of hybridoma cells, producing a monoclonal antibody, was monitored for 4 and a half days, and the results obtained are listed in the following table: time (days) nº cel/mL x 106 ln nº cel 0 0,45 -0,79851 0,2 0,52 -0,65393 0,5 0,65 -0,43078 1 0,81 -0,21072 1,5 1,22 0,198851 2 1,77 0,57098 2,5 2,13 0,756122 3 3,55 1,266948 3,5 4,02 1,391282 4 3,77 1,327075 4,5 2,2 0,788457 What is the maximum specific rate (µmax) of growth of these cells per day? what is the doubling time of these cells (in days)?
5b
What is the experimental procedure illustrated in the figure above? What label (1-3 words) would you write next to each of the 5 arrows in the procedure? ) Briefly write a concise but complete description of the figure closely following the labels you have used in your answer to the second part of the question.
To identify a positive control in your lab report, should you list any test tube with positive results? Why or why not? What is a negative control? Which substance is a negative control for each of the tests in this lab exercise?

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Which among the following statements is correct? O lon-exchange chromatography is dependent on the charge and size of the target component Affinity chromatography is dependent on the affinity of the target component to the mobile phase Both statements are correct Both statements are incorrect