Which among the following statements is correct? O lon-exchange chromatography is dependent on the charge and size of the target component Affinity chromatography is dependent on the affinity of the target component to the mobile phase Both statements are correct Both statements are incorrect

Plate A = filter paper dipped into 5% solution of unknown Dye A %3D Plate B = filter paper dipped into 5% solution of unknown Dye B Both plates were incubated at 37°C. At the end of the 60 minute incubation period the diffusion rings for each dye were measured. The diffusion ring for Dye A = 40 mm. The diffusion ring for Dye B = 24 mm %3D Based on the results, which dye had the higher molecular weight? Explain your answer.

uring dialysis, a semi-permeable membrane with a molecular weight cutoff of 10,000 is used. Select the true statements from the list below (more than one answer could apply) Group of answer choices A monoclonal antibody with a 150 kDa MW is retained and does not diffuse across the membrane A small enzyme with 20 kDa MW diffuses readily across the membrane Buffer salts with molecular weights ranging between 100 and 500 Da diffuse across the membrane until equilibrium (equal concentration) is reached on both sides of the membrane None of the above statements are correct Please answer asap

1) If direct stains work so well, why would we ever use indirect stains? Give two reasons why you might choose to use indirect stains instead of direct stains?  2) Assume that two days (dye A and dye B) were applied to bacterial smear. The cells were stained by dye A while the slide background was stained with dye B. Which stain had an anionic chromogen? Which stain had a cationic chromogen?