Propidium iodide (PI) can be used to study both cell division and apoptosis using flow cytometry. Describe how PI is used to study the two cellular events with the principles explained. You are expected to give the full principle of the flow cytometric analysis of apoptosis.
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Propidium iodide (PI) can be used to study both cell division and apoptosis using flow cytometry. Describe how PI is used to study the two cellular events with the principles explained. You are expected to give the full principle of the flow cytometric analysis of apoptosis.
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- A Manton-Gaulin homogenizer is used for cell lysis. Which of the following are true? Increasing the number of passes can increase fine cell debris and make subsequent clarification more difficult. Cell growth conditions and phase of growth at which cells are processed can impact cell disruption. Decreasing temperature can increase protein release. Decreasing pressure reduces the number of passes required. Explain what is meant by the extent of cell disruption is 0.9.For most cell types, serum must be added to the media to get cells to grow and proliferate in culture. Nevertheless, serum is very expensive and can complicate experiments utilizing the cells. Many companies have developed "artificial serum" which enables cells to multiply without the use of serum. See data below. What are likely components of the artificial serum that enables cells to grow. Explain (limit 3-4sentences). Non 2% Human plasma 1% Artificial serum(XF) 1% Artificial serum(AF) 104 cells/cm² Cell density cultured after 4 days 127 10- 2 0 NON 2% 1% 1% Human Artificial Artificial plasma serum(XF) serum(AF) PDL [times] 30- 25+ 20- 15- 10- 5- 0 -2% Human plasma 0 Subculture 1% Artificial serum (XF) 1% Artificial serum(AF) 5 10 15 20 25 30 Culture days Medium: HFDM-1 (+) was used.If somatic tumors cause local regions in their vicinity to have an increased pOH, how is this strategic for the survival of the tumor? Select one: a. It allows the increased hydroxide concentrations to cause a conformational change in nearby hemoglobin to the R state so that ample oxygen is deposited for tumor growth. b. It allows the increased proton concentrations to cause a conformational change in nearby hemoglobin to the R state so that ample oxygen is deposited for tumor growth. c. It allows the increased proton concentrations to cause a conformational change in nearby hemoglobin to the T state so that ample oxygen is deposited for tumor growth. d. It allows the increased hydroxide concentrations to cause a conformational change in nearby hemoglobin to the T state so that ample oxygen is deposited for tumor growth. e. None of these.
- Outline an experimental approach to measure Amyloid Beta that microglia produces in vitro. What are some of the problems you might encounter?Dr. Jace is a research officer in a laboratory that studies anticancer treatment. She is interested to test a plant extract for its apoptosis-inducing effect on colon cancer cells. After incubating the cells with the extracts, she is using the FITC Annexin V Apoptosis Detection Kit I by BD Biosciences for her apoptotic assay. Discuss the detection of apoptosis using this kit and include the utilization of flow cytometry in her experiment.Cancer stem cells (CSCs) are cancer cells (found within tumors or hematological cancers) that possess characteristics associated with normal stem cells, specifically the ability to give rise to all cell types found in a particular cancer sample. There are many biomedical engineering based approaches to detect CSCs. Question: What is the importance and advanatge of detecting CSCs? Please explain in detail
- Discuss/explain, in details, the relationship between cell viability and cell vitality and cell apoptosis as suggested by neutral red uptake,Cancer stem cells (CSCs) are cancer cells (found within tumors or hematological cancers) that possess characteristics associated with normal stem cells, specifically the ability to give rise to all cell types found in a particular cancer sample. There are many biomedical engineering based approaches to detect CSCs. Question: What is the importance and advanatge of detecting CSCs?Cancer stem cells (CSCs) are cancer cells (found within tumors or hematological cancers) that possess characteristics associated with normal stem cells, specifically the ability to give rise to all cell types found in a particular cancer sample. There are many biomedical engineering based approaches to detect CSCs. Question: What is the importance and advanatge of detecting CSCs? Please explain in detail the main findings with your own words.
- Explain the following figure. According to this model, what are the two major phases of metastasis that must be successful for metastasis? What are the challenges faced by cancer cells during metastatizing?Based on the data in Table 3 above, prepare the following scatter plot graph. a. On the same graph, you should include the following two lines : i. Cell surface area on the x-axis and the 1:1 surface area ratio on the y-axis (these values will be identical yielding a diagonal line with a slope of 1). ii. Cell surface area on the x-axis and cell volume on the y-axis. b. Based on a comparison of these two curves and your data in Table 3, at what approximate range of points (side length of a cell) does cell volume begin to exceed surface area? Explain the implications beyond this range for cells.Taxol, derived from the Pacific Yew tree, was one of the first effective anti-cancer compounds discovered. It acts by inhibiting microtubule depolymerization. Which of the following is a likely cellular side effect of treatment with Taxol? Increased exocytosis Loss of pseudopod formation in white blood cells Decreased endocytosis Increased cell size