For most cell types, serum must be added to the media to get cells to grow and proliferate in culture.Nevertheless, serum is very expensive and can complicate experiments utilizing the cells. Many companieshave developed "artificial serum" which enables cells to multiply without the use of serum. See data below.What are likely components of the artificial serum that enables cells to grow. Explain (limit 3-4sentences).Non2% Human plasma1% Artificial serum(XF) 1% Artificial serum(AF)104 cells/cm²Cell density cultured after 4 days12710-20NON2%1%1%Human Artificial Artificialplasma serum(XF) serum(AF)PDL [times]30-25+20-15-10-5-0-2% Human plasma0Subculture1% Artificial serum (XF)1% Artificial serum(AF)510 15 20 25 30Culture daysMedium: HFDM-1 (+) was used.

In humams serum is the plasma without clotting factors. Which means that plasma contains antibodies,…

Answered: For most cell types, serum must be…

Microbiology for Surgical Technologists (MindTap Course List)

2nd Edition

ISBN:9781111306663

Author:Margaret Rodriguez, Paul Price

Publisher:Margaret Rodriguez, Paul Price

Chapter5: The Eukaryotes

Section: Chapter Questions

Problem 2UTM: Sterile normal saline is used in most surgical procedures as an irrigation solution. During certain...

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A Manton-Gaulin homogenizer is used for cell lysis. Which of the following are true? Increasing the number of passes can increase fine cell debris and make subsequent clarification more difficult. Cell growth conditions and phase of growth at which cells are processed can impact cell disruption. Decreasing temperature can increase protein release. Decreasing pressure reduces the number of passes required. Explain what is meant by the extent of cell disruption is 0.9.
Assume that you are starting with a sample containing 6 billion active cells. Create a dilution plan to reduce this count to a range of 30-300 colonies on a plate. The powdered sample weighs 500 mg (0.5 g). You can use up to 4 plates – you should plate different dilutions and/or volumes of solution. Start your first dilution by adding 10 mL diluent to 0.5 g powder, which is a 1/20 dilution. Hint: Rearranging the “cfu/ml” formula from our dilutions lab can help you solve this. Choose a target cfu count, and your volume plated should be under 0.5 ml.
Propidium iodide (PI) can be used to study both cell division and apoptosis using flow cytometry. Describe how PI is used to study the two cellular events with the principles explained. You are expected to give the full principle of the flow cytometric analysis of apoptosis.
The ability of cells suspended in soft (semi-solid) agar medium to form colonies is a good predictor of which of the following? (select all that apply)? A. The cells have lost contact inhibition B. These cells will form tumors if injected subcutaneously into immunocompromised mice. C. These cells have stopped proliferating and will die. D. These cells have an increased requirement for growth factors. E. These cells would fail to grow in normal medium.
Density gradient centrifugation is an inexpensive cell separation technique. Mention 2 limitations of this separation technique
Beginning with 10 grams of plant tissue, order the following procedures (as steps 1-4) that you will use to obtain a cell fraction that consists of mostly large cell organelles: collect pellet, collect supernatant, high-speed centrifugation for 10 min., low-speed centrifugation for 5 min, filter homogenate, grind plant tissue Step 1 grind plant tissue | Choose collect supernatant low-spccd centrifugation for 5 min high-speed centrifugation for 10-min collect pellet grind plant tissue filter homogenate Step Step 3 Step 4 high-speed contrifugation fo v
How does the microscopy in Figure 2 show that the capsule and an S-layer can exist in the same cell at the same time? I need help finding the answer in the article and explain in short answer link to article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/
Sterile normal saline is used in most surgical procedures as an irrigation solution. During certain surgical procedures, such as those for malignancy, the surgeon may request that sterile water be used for irrigation instead of saline following excision of the cancerous mass. Why is normal saline usually used in healthy tissues?
Immunofluorescence and Fluorescent livecell imaging techniques can both be used to determine protein localisation.List the advantages and disadvantages of using immunofluorescence andfluorescent live cell imaging
You are planning an experiment to treat cells in culture with compound A at a final concentration of 10 µM. The stock solution concentration of compound A is 1 mM. What two controls should you have in your cell culture experiment using compound A? Explain both the type of controls and the concentration or volume of the treatments.
We need to prepare a stock solution of medium for your culture cells, which usually includes liquid salt solution and bovine serum. Our liquid salt solution is supplied in a 50X concentration, and we need to dilute it to 1X for use. We also need to add 75% fetal bovine serum for a final concentration of 15%. How would we make up 0.80 liters of this culture media using water as our solvent?
You are doing a viable cell count for a culture of E. coli you are growing in a flask. The volume of media in the flask is 1L. You took an aliquot directly from the media and diluted it using trypan blue. Here are the numbers that you get: squares counted: 4 total cells counted: 988 total viable cells counted: 960 THESE ARE THE QUESTIONS: What is your cell viability? What is the total number of viable cells in your flask? Show all work. Given: Cell Viability = Viable Cells/Total Cells Cells/mL = Total Viable Cells/Coners Counted x 10,000 x Dilution factor

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