A man feels a shooting pain in his arm, then a thundering in his chest. Realizing that he is in the throes of a heart attack, he reaches for his self-injector of tPA (tissue plasminogen activator) and quickly injects himself. The tPA begins to break apart the blood clots that are blocking his heart’s circulation. This lifesaving protein is naturally found in the human body in tiny amounts. The man’s tPA drug, although identical to his own, was manufactured in bacteria. a. How is it possible for bacteria to express protein that was coded for by a human gene? b. Due to certain advances in biotechnology, it became much cheaper to produce tPA. What DNA technology made it possible to produce large quantities of this protein in bacterial cells?

A man feels a shooting pain in his arm, then a thundering in his chest. Realizing that he is in the throes of a heart attack, he reaches for his self-injector of tPA (tissue plasminogen activator) and quickly injects himself. The tPA begins to break apart the blood clots that are blocking his heart’s circulation. This lifesaving protein is naturally found in the human body in tiny amounts. The man’s tPA drug, although identical to his own, was manufactured in bacteria. a. How is it possible for bacteria to express protein that was coded for by a human gene? b. Due to certain advances in biotechnology, it became much cheaper to produce tPA. What DNA technology made it possible to produce large quantities of this protein in bacterial cells?

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter17: Genes And The Immune System

Section: Chapter Questions

Problem 7QP

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(e) Two ADP agonists (drugs) were also found to bind to platelets: 2-methylthio-ADP bound with a Kp of 7 µM and 2-(3-aminopropylthio)-ADP bound with a Kp of 200 µM. Can these drugs effectively compete with ADP for binding to platelets? Explain your answer.
A patient has had a serious accident and lost a lot of blood. In an attempt to replenish body fluids, a large amount of distilled water was transferred directly into one of his veins. What will be the most probable result of this transfusion? It will have serious, perhaps fatal, consequences because the red blood cells will be hypertonic relative to the body fluids and the cells will burst. It will have serious, perhaps fatal, consequences because the red blood cells will be hypotonic relative to the body fluids and the cells will shrivel. It will have no unfavorable effect as long as the water is free of viruses and bacteria. It will have no serious effect because the kidneys would quickly eliminate the excess water. It will have serious, perhaps fatal, consequences because there will be too much fluid for the heart to pump.
Bence-Jones protein is a protein with a molecular weight of approximately 150 kDa and is found in high concentration in the urine of Multiple Myeloma patients. Multiple Myeloma is a type of cancer in which lymphocytes multiply and break down bone marrow and bone. Which method would you prefer to show the Bence-Jones protein in the urine for the diagnosis of the disease? Explain the principle of your preferred method.
The anticlotting property of heparin is partly the result of the negative charges it carries. ( Q.) Which type of heparin is a better anticoagulant, one with a high or a low degree of polymerization?
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You want to understand how the density of P-selectin in blood vessel walls affects the rolling interactions of neutrophils when they are subjected to hydrodynamic drag forces in the blood. You introduce P-selectin into a synthetic lipid bilayer and attach it to a glass slide mounted in a flow chamber. This arrangement allows you to measure neutrophil attachment at different densities of P-selectin and at different flow rates. At high densities, from 30 to 400 P-selectin molecules per µm², the neutrophils attached to the membrane and rolled very slowly and jerkily in the direction of the flow (see cell 1 in the figure). At the same flow rate, but at a density of 15 P-selectins per µm², the neutrophils behaved differently: they either moved at the flow rate of the medium or were transiently tethered-horizontal lines marked by t-before moving again (cell 2 in the figure). Neutrophils showed this same behavior at a density of 1 P-selectins per μm² and half the flow rate: moving at the flow…