When an EcoR1 fragment, which represents the coding region of a human gene X, is cloned into the EcoR1 site upstream of the coding region of a prokaryotic gene (such as GST) (i.e., to make a X-GST fusion protein), what is the chance of an in-frame fusion? Please draw a diagram to explain your answer. Do you need to delete the stop codon of the X gene coding region before fusing it to the GST coding region? Please draw a diagram to explain your answer. Notes: It is NOT known which strand of the human gene X is the template strand for transcription. 2) Both X and GST protein fragments must be produced correctly)

Suppose you have a 1-kb segment of cloned DNA that is suspected to contain a eukaryotic promoter including a TATA box, a CAT box, and an upstream GC-rich sequence. The clone also contains a gene whose transcript is readily detectable. Your laboratory supervisor asks you to outline an experiment. (1) determine if eukaryotic transcription factors (TF) bind to the fragment and, if so, (2) identify where on the fragment the transcription factors bind. All necessary reagents, equipment, and experimental know-how are available in the laboratory. Complete the outline of an experiment, which determines if eukaryotic transcription factors (TF) bind to the fragment. Assume all necessary reagents, equipment, and experimental how are available in the laboratory. Drag the terms on the left to the appropriate blanks on the right to complete the sentences. Not all terms will be used. For the band shift assay, two samples of the DNA fragment are analyzed by agarose gel electrophoresis: one sample is…

Choose 1 or more than 1 RNA are extracted from liver cells and separated in agarose gel by electrophoresis side-by-side with a molecular weight marker. The separated RNA fragments are then transferred to an RNA-binding membrane. Next, this membrane is incubated with labelled probe specific for the gene X. This experiment determines:   how many copies of gene X there are in liver cells. if the gene X is translated in liver cells. if gene X has a point mutation in liver cells. the chromosomal location of gene X. the length of the transcript of gene X.

The CRISPR-Cas9 system may be able to be used for somatic gene therapy to treat terminal genetic diseases such as Duchenne Muscular Dystrophy (DMD), which affects 1 in 3,500 male births worldwide. As we talked about in class, DMD results from a nonsense mutation that causes a premature stop codon in Exon 23 of the largest gene yet discovered in the human genome, the 79- exon dystrophin gene. Of the choices below, which would be most likely to restore the protein and muscle function? O A deletion of exon 23 O A deletion of intron 23 O A deletion of intron 23 and exon 23 O An insertion of a corrected exon 23 upstream of the mutant exon 23 O A deletion of exons 23, intron 23, and exon 24

As a new graduate student, you are tasked with studying patients with Pitt - Hopkins syndrome, caused by mutations in the basic helix -loop-helix transcription factor TCF4. Further, you know that TCF4 is a homodimer and that proper function requires dimerization, in addition to subsequent DNA binding. To begin your study, you sequence the TCF4 gene in your three patient cohort and identify several sequence variants. The TCF4 coding region and sites of mutations are shown here (lines between codons indicate the correct reading frame). classify each of these mutations on the coding sequence (1 mark each); most likely impact on the resultant protein (1 mark each); and how each might affect ability of TCF4 to influence transcription (1 mark each). Patient 1 mutation: Patient 1 protein: Patient 1 functional effect: Patient 2 mutation: Patient 2 protein: Patient 2 functional effect: Patient 3 mutation: Patient 3 protein: Patient 3 functional effect: Patient #3 Patient #3 CTA LAGT GGT Wild…

A newly identified protein from the  cells of the Panopyra plant on Pandora was shown to inhibit translation of its target genes by binding to the 5’ UTR of the mRNA and preventing ribosome binding.  A possible way this inhibition may be relieved by an sRNA would be:  Group of answer choices a)The sRNA acts as a silencer, suppressing the inhibitory protein and allowing translation to take place. b)The sRNA acts as a decoy, sequestering the inhibitory protein and allowing translation to take place. c)The sRNA acts as a marker, flagging the inhibitory protein for ubiquitination and allowing translation to take place.

When a region of DNA that contains the genetic information for a protein is isolated from a bacterial cell and inserted into a eukaryotic cell in a proper position between a promoter and a terminator, the resulting cell usually produces the correct protein. But when the experiment is done in the reverse direction (eukaryotic DNA into a bacterial cell), the correct protein is often not produced. Can you suggest an explanation?

Gene X codes for a protein in eukaryotes. A mutated eukaryotic cell contains an altered base-pair in an intron of gene X. Which would be the most likely effect of this mutation on the biomolecules in the cell? The amount of pre-mRNA transcribed from gene X would be less than normal.  The amount of functional protein corresponding to gene X would be less than normal.  The ability of snRNAs to form a spliceosome would be diminished. The breakdown of mature mRNA corresponding to gene X would be faster

Which construct would you use to make a mouse model ?

Suppose you had isolated a new transcription factor and wanted to know which genes this protein might regulate. Is there any way that you could use a cDNA microarray of the type shown in the picture to approach this question?