Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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Question
Please answer both
A. In an E. coli gene that uses rho dependent
termination, the rut site has been deleted. What effect
will this have on transcription of the gene? Explain your
answer using scientific terms.
B). The genetic map of the akt gene is as follow:
300bp
200bp
100bp
200bp
6000bp
akt gene map
To study the akt gene, you extracted RNA from chicken
cell cytoplasm (Sample 1) and nuclei (Sample 2). You
ran these two RNA samples on a gel, transferred them
to a membrane and detected the akt mRNA with a
labelled probe. Answer the following questions:
a. What is this technique called?
b. Draw the bands as they would appear on the
membrane. Explain.
MWM
Sample 1
Sample 2
bp
10 000
8 000
-
6 000
5 200
3 500
-
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Transcribed Image Text:A. In an E. coli gene that uses rho dependent termination, the rut site has been deleted. What effect will this have on transcription of the gene? Explain your answer using scientific terms. B). The genetic map of the akt gene is as follow: 300bp 200bp 100bp 200bp 6000bp akt gene map To study the akt gene, you extracted RNA from chicken cell cytoplasm (Sample 1) and nuclei (Sample 2). You ran these two RNA samples on a gel, transferred them to a membrane and detected the akt mRNA with a labelled probe. Answer the following questions: a. What is this technique called? b. Draw the bands as they would appear on the membrane. Explain. MWM Sample 1 Sample 2 bp 10 000 8 000 - 6 000 5 200 3 500 -
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