You design a guide RNA to target your gene of interest, which you will express attached to a scaffold RNA. You transform cells with Cas9, the guide+scaffold RNA, and an HDR template to introduce a mutation into the target sequence (a 3 bp deletion located 5 bp upstream of the Cas9 cut site and a base pair substitution 15 bp upstream of the Cas9 cut site). Which of the outcomes below are possible? Type possible or not possible in each answer box. Answer: A. Cas9 does not cut target sequence B. Cas9 cuts target, 10 bp deletion introduced C. Cas9 cuts target, no mutations introduced D. Cas9 cuts target, desired 3 bp deletion introduced but not the substitution E. Cas9 cuts, 3 bp deletion and substitution introduced

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Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.