You design a guide RNA to target your gene of interest, which you will express attached to a scaffold RNA. You transform cells with Cas9, the guide+scaffold RNA, and an HDR template to introduce a mutation into the target sequence (a 3 bp deletion located 5 bp upstream of the Cas9 cut site and a base pair substitution 15 bp upstream of the Cas9 cut site). Which of the outcomes below are possible? Type possible or not possible in each answer box. Answer: A. Cas9 does not cut target sequence B. Cas9 cuts target, 10 bp deletion introduced C. Cas9 cuts target, no mutations introduced D. Cas9 cuts target, desired 3 bp deletion introduced but not the substitution E. Cas9 cuts, 3 bp deletion and substitution introduced

Q: One can synthesize RNA from any gene in a test tube (in vitro). You want to synthesize mRNA from a…

A: Translation is a heterocatalytic action of DNA by means of which RNA is synthesize from specific DNA…

Q: The tac promoter, an artificial promoter made from portions of the frp and lacUV5 promoters, has…

A: The lac operon is an operon required for the transport and metabolism of lactose in E.coli and many…

Q: O a base substitution in an intron 5' splice site O a frameshift in an exon O a missense in an exon…

A: The answer to the question is  A frameshift in exon

Q: You are studying a human gene, and try to express the protein in E. coli bacterial cells. To do…

A: Cloning is the process of creating a genetically identical copy of a biological entity. Cloning can…

Q: Consider the Rho-dependent terminator sequence 5’CCCAGCCCGCCUAAUGAGCGGCCUUUUUUUU-3’. What affect…

A: The mutation is the change in the nucleotide sequence of the DNA. The point mutation is the mutation…

Q: Mutation in the operator that reduces the affinity of the operator for the repressor protein…

A: Lac operon is an inducible operon. When lactose is present in medium, then lac operon starts.

Q: The best vector to use determine receptor binding protein expression would have been one with a GFP…

A: a) GFP is a green flourescent protein whose flourescence can be observed under microscope so, a…

Q: Propose a final method of gene regulation of the Up Lateoperon using an updated drawn figure of the…

A: An operon is a part of the DNA where a few genes are clustered together that are transcribed…

Q: A full-length eukaryotic gene is inserted into a bacterial chromosome. The gene contains a complete…

A: The eukaryotic DNA is composed of coding and non-coding sequences, almost 90% are non-coding…

Q: A graduate student studying yfgA expression in the liver and heart performs an RNA-seq experiment.…

A: In order for a gene to produce functional gene products, such as proteins or non-coding RNA, and…

Q: sRNA acts as a marker, flagging the inhibitory protein for ubiquitination and allowing translation…

A: Proteins are synthesized by the process of translation and are modified after post-translational…

Q: If you wanted to express a library of human proteins in yeast, there are several good reasons why it…

A: The objective of the question is to identify the option that is not a valid reason for preferring a…

Q: The lac operon consists of three structural genes, lacZ, lacY and lacA that are transcribed as a…

A:

Q: Which of the following statements is true regarding the lys2-128d reporter?  Select all that apply.…

A: INTRODUCTION Lys2-128d is a reporter allele of the Spt- gene in Saccharomyces cerevisiae (yeast)…

Q: What is a consensus sequence? How similar to the consensus sequence would you expect the promoter of…

A: Introduction  An area of DNA upstream of a gene called a promoter, as it relates to genomics, is…

Q: During experimental RNAi, how does the researcher affect expression of a target gene? Group of…

A: Introduction: In the process of RNA interference(RNAi) double-stranded RNA molecule suppresses the…

Q: What would be the consequence of inserting 10 random base pairs immediately downstream of the -10…

A: The insertion of 10 random base pairs immediately downstream of the -10 box in a strong promoter may…

Q: The mRNA used for the SARS-CoV-2 vaccine from Pfizer/BioNTech is generated in a cell-free system by…

A: The hereditary molecule deoxyribonucleic acid (DNA) transmits genetic information from one…

Q: A series of exonuclease deletions were used to study the promoter of the rice hemA gene, giving the…

A: A promoter can be defined as a DNA sequence where proteins bind and initiate transcription of an…

Q: The Cas9 enzyme requires a short sequence called a PAM (Protospacer Adjacent Motif) for cleaving the…

A: A protospacer adjacent motif (PAM) is a 2–6 base pair DNA sequence tareted by Cas9 nuclease.

Q: The lac operon consists of three structural genes, lacZ, lacY and lacA that are transcribed as a…

A: 1) Allolactose inhibits the repressor, allowing the RNA polymerase to bind to the promoter and…

Q: After comparing the whole genome sequencing results from a candidate that you suspect has a mutation…

A: A missense mutation is a type of genetic mutation that results in the substitution of one nucleotide…

Q: Some organisms have mechanisms in place that will override transcription termination. One such…

A: The HIV-1 (Human Immunodeficiency virus-1) Tat (transactivator of transcription) gene codes for a…

Q: In the Avery, McLeod, McCarty Experiment where supernatant from heat killed, virulent S Strain…

A: Avery, McLeod and McCarty experiment was used to determine the genetic material and its nature on an…

Q: A rice MADS box transcription factor gene contains a number of sequences that are removed in the…

A: In eukaryotes including rice mRNA processing involves the removal of introns which are non-coding…

Q: In your own words, explain the term transposon tagging.

A: Transposons or jumping genes are the sequences of DNA that are able to change their position within…

Q: You run a Western blot to detect Ras in sampe A and sample B. You find that sample B gives a darker…

A: First of all, Western Blot is a technique which is used to detect the expression of protein from…

Q: (b) Bacteriophage 434 repressor SIN Which of the following is true regarding the interaction of the…

A: The 434 repressor is a protein that plays a key role in the regulation of gene expression in the…

Q: You obtain the sequence of a gene containing 10 exons, 9 introns, and a 3'-UTR containing a…

A: A gene is a stretch of nucleotides present on a chromosome. It is the region that encodes the…

Q: E. coli strain BW25113 can grow on (D) Arg as the sole carbon source. A scientist  working with you…

A: Obtain the genomic  from the mice cells that encode for the protease of interest.Fragment the…

Q: How will this modified cro protein interact with the three OR sites, and how would cro expression be…

A: Bacteriophages are viruses that infect bacteria. Like other viruses bacteriophages are also made up…

Q: The IMD2 promoter contains three upstream transcription start sites (TSS) that are utilized under…

A: Inosine monophosphate dehydrogenase (IMD2) is a key enzyme in the purine biosynthesis pathway that…

Q: How would you use a ChIP (chromatin immunoprecipitation) to show that when RNA pol II is bound at…

A: Chromatin immunoprecipitation is a technique used for the investigation of DNA (deoxyribonucleic…

Q: The carboxy terminus of the p53 protein acts as an allosteric regulator of sequence-specific DNA…

A: General structure of p53 protein

Q: The lactose operon in E. coli comprises two structural genes and three regulatory components.…

A: β-galactosidase activity Permease activity Genotypes Glycerol Lactose Glycerol Lactose A - + -…

Q: Which of the following statements is not true about the guide RNA (see this interactive…

A: The objective of the question is to identify the incorrect statement about guide RNA in the context…

Q: The following diagram show what is required for an active promoter of a gene of interest, where:A1 =…

A: Based on the given diagram, here are the predictions:Chromatin conformation: EuchromatinMethylation…

Q: You have isolated a mutant that you call Mutant Z. In a Mutant Z strain, the RNA polymerase can bind…

A: Here, the correct answer is option c) domain 2 of sigma and d) 3/4 linker of sigma.

Q: Explain how a transcriptional fusion to a reporter gene, combined with site directed mutagenesis,…

A: Studies of gene expression and promoter activity are frequently made easier with the aid of gene…

Q: ou were tasked to develop two types of vaccines: a DNA vaccine and a protein subunit vaccine. For…

A: Recombinant DNA Vaccine : Live attenuated vaccine : These are the vaccines where whole bacteria or…

Q: After decades of work, Dr. Ricky M. isolated a small amount of attractase—an enzyme that produces a…

A: A gene is the essential physical and functional unit of heredity. Genes are comprised of DNA. A…

Q: To create a cDNA library, cDNA can be inserted into vectors and cloned. In the analysis of cDNA…

A: A gene is an area of DNA molecule or polymer of amino acid that encodes function. A chromosome…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.