Using saline tubes containing 9 ml of saline (for dilutions), draw a diagram that would yield between 30 and 300 colonies from an original broth culture that contains 5 * 10^8 cells/ml. Indicate volumes used and dilutions made for each tube and plate.

Q: What is the dilution factor in the task given if the total viable cells is 140 [28×5(squares)] and…

A: The dilution factor is a measure of how much a solution has been diluted or concentrated. It is…

Q: Determine what percentage of the culture was living (viable) and what percentage was dead…

A: The word "CFU" in microbiology refers to a colony-forming unit, which is used to estimate how many…

Q: Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL…

A: Serial dilution is a progression of successive dilutions used to lessen a thick culture of cells to…

Q: A culture is incubated for 10 hours. 1 hours after inoculation it reached the exponential growth…

A: This question involves the calculation of the growth constant (k) and generation time (g(t)) for a…

Q: A culture of E. coli has a concentration of 5 x 108 cells/mL. How many times do you have to dilute…

A: Given: 1. Number of colonies: 2502. No. of bacteria in culture: 5 X 108 3. Volume plated: 0.1 ml 4.…

Q: The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial…

A: Culture broth is a liquid medium that is used to grow microorganisms, such as bacteria, fungi, and…

Q: transferred to the plate. After 24 hours there were 60 colonies on the plate. What is the dilution…

A: b. The dilution factor is 1000. The patient sample contains 600,000 cells/ml

Q: Using one of the graphs obtained with the medium II, name the different phases of the bacterial…

A: Micro organisms are typically grown in a closed culture media. Nutrients are added according to the…

Q: This is the growth curve for Clostirdium (incubated under optimal growth conditions). Use it to…

A: There are 5 phases to a logistic (sigmoidal) growth curve.  They are stationary phase, lag phase,…

Q: Describe how you would execute this first stage from the point you are handed the nutrient broth…

A: For isolation of any culture, it needs to be incubated in broth to obtain full fledged turbidity or…

Q: Answer the following questions based on Kirby Bauer / MacFarland standards 1-0.5 McFarland…

A: The Kirby Bauer test, also known as the disc diffusion test, is a common laboratory technique for…

Q: In this experiment, a culture was serially diluted to the concentrations below. Each plate was…

A: A "microbe" is a living entity that is so tiny that it cannot be seen with the naked eye.…

Q: Assume that you are adding 300 microliters of 1% substrate solution per well in a 24-well plate. If…

A: Fibronectin is a glycoprotein found in the extracellular matrix of cells and is also obtained from…

Q: The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial…

A: Colony forming unit refers to the measurement of the number of viable cells present in per ml of the…

Q: You are given a bacterial culture which has a concentration of approximately 5.0 x 10^8 cells/mL.…

A: We are given a bacterial culture containing approximately 5.0 x 108 cells/mL. To identify the exact…

Q: You prepared a 7x 10^5x dilution from your bacterial culture, plated 0.2 ml of it on a Petridish and…

A: A colony-forming unit (cfu) is a unit used in microbiology to estimate the number of viable bacteria…

Q: Drawings of the two bacterial cultures as observed under the 4 mm objective. Describe features…

A: Staphylococcus aureus and Streptococcus pyogenes are gram positive bacteria that cause infections in…

Q: If each agar plate contained 20 ml of LB agar and had an ampicillin concentration of 150 ug/ml, what…

A: Given data:  Each plate contains-  LB agar=20ml Ampicillin= 150 micrograms per ml.  Amount of…

Q: You need to prepare medium for your culture cells. Your salt solution is supplied at a 10X…

A: The question asks to find the final concentrations of the following in 5 L of media:The salt…

Q: Below is a set of data for a serial dilution/plate count experiment.    Based on this data what is…

A: There are numerous methods/techniques for counting the number of bacteria in a particular sample. A…

Q: where 5 nutrient agar pates per female student and 3 agar slants per males student are needed to…

A: It is given that 5 nutrient agar plate prepared by per female so Seven female will prepare=7*5=35…

Q: ) Gel filtration chromatography on Sephadex G50 b.SDS-PAGE

A: Gel filtration: with this method we could separate  protein with respect to molecular weight in the…

Q: You count 4 squares of a hemacytrometer and ontain 82,65,85 cells in each squy.what is the cell…

A: Hemocytometer is device used for the counting of blood cells in a sample. It has counting chambers…

Q: Refer to the provided image drawn by a student trying to plan out their serial dilution protocol.…

A: Colony forming unit (CFU)is a measure of viable bacterial cell. CFU/ml can be calculated using…

Q: A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to determine…

A: To keep the number of colonies on the plate within a certain range, serial dilutions of culture are…

Q: You perform a serial dilution of a water sample and the 10-7 tube when 0.1 ml was plated onto the…

A: Estimation of the number of viable cells is important in microbiology protocols. It is estimated…

Q: colony-forming units

A: Colony Forming Unit:It is designated as CFU.It can defined as the unit of measurement used to…

Q: Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you…

A: Cfu refers to the colony forming unit which is formed through continuous replication of a single…

Q: If there is 50 bacterial colonies on a 1:1000 dilution streak plate. How many cfu/mL are there?

A: Introduction: A CFU stands for colony-forming units. It is a unit that we use for estimating the…

Q: Using the attached image, explain the observed results on the NA and ECM plates. Be sure to address…

A: Bacteria are prokaryotic, unicellular organisms. They belong to the kingdom Monera. They do not have…

Q: For the following agar media used, list their significant components and explain how they react.…

A: The sheep blood agar is a popular agar medium. It is a differential medium that includes important…

Q: Given the scenario, compute for the total volume of the culture media solution (milliliter or liter)…

A: We have been given the concentration of the nutrient broth and Agar and we have also been given the…

Q: If you have a 15 mg/100 ml stock solution of GA3 and you need a 1 mg GA3 in 25 ml, how much stock…

A: Given: volume of stock solution is 15 mg/100 ml Needed amount is 1 mg kinetin in 25 ml Volume of…

Q: At the station there is a 2 mL microcentrifuge tube containing sheep blood and 3 tubes containing…

A: Homolysis or hemolytic cleavage is the cleavage of chemical bond usually in the presence of light or…

Q: You are determining the concentration of your culture, and have counted the following on your…

A: Hemocytometer is a glass slide at with margins on its surface which is used for counting the number…

Q: A urine sample is diluted 1:1,000. If 0.1 ml were put into a Petri dish with melted agar, how  many…

A:

Q: zone size

A: Antibiotics:These are the medications used to treat the bacterial infections. The appropriate…

Q: You are interested in the total bacterial load of the bat guano. In order to determine this, you go…

A: For calculation of total bacterial count we choose the solution that usually gives the colony number…

Q: The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial…

A: Here, we will explore the process of determining the number of bacterial colony-forming units (CFUs)…

Q: You just finished plating your electroporatio products on your YPD-Zeocin plate and you think you…

A: Electroporation is a technique used to introduce foreign DNA into cells by applying an electric…

Modes of Nutrition

It is a biological process of providing or obtaining food that is necessary for growth and health. It includes many physiological processes - absorption, ingestion, assimilation, catabolism, excretion, etc. In biology, the science that deals with nutrition's physiological and chemical processes are called "nutritional science."