Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you make this mistake. 1.You intend to add 9ml of broth (water) to each tube and 1 ml of culture. Instead, you add 5 ml of broth to each tube and 5 ml of culture to the first tube. Then, you make a serial dilution of 1 ml into and from each tube as described. 2. You prepare 9 ml of broth (water) in each tube. You add 1ml of culture of every tube. 3.You add 9 ml of broth (water) to each tube. You add 1 ml of culture to the first tube and mix. You get distracted, and transfer 1ml of the dilutant (mixture of culture and broth) to the third tube instead of the second. You perform the rest of the series as described.

Create a diagram of the steps in the Most Probable Number test. You may insert a computer-made diagram, or you may draw a diagram by hand

1. You wish to determine viable counts on a culture of Bacillus subtilis. You begin by pipetting 1 ml of culture into 99 ml of sterile water. After mixing the dilution well you make a series of 4 further dilutions of 10-1 each. From the three most dilute samples, you prepare three pour plates using 1 ml in each. After incubation you find the plate counts of the plates are 16, 245 and 890 respectively.  a) Show the dilution scheme. b) What is the estimated viable count (cells/ml) in the original culture?   2. Scientific Notation. Fill in the missing information. a) 4.5 x 109 = _______  b) 50 x 107 = _______ c) 2300 x 1010 = _______ d) 0.54 x 108   = _______

Draw the steps of Qualitative Slide Test in LATEX AGGLUTINATION TEST FOR THE DETECTION OF RHEUMATOID FACTOR: SCREEN AND TITER 1. Bring all reagents and test samples to room temperature before use. 2. Make a 1:20 dilution of the patient serum by pipetting 0.1 ml of patient serum into 1.9 mL of 1X diluent or saline in a test tube. Pipet 50 µl of the diluted sample onto one test circle on the slide. 3. Place one drop (about 50 mL) of positive control onto a separate test circle and one drop of negative control onto another test circle. 4. Invert the latex reagent bottle several times to mix the beads into the suspension. Add one drop of well-shaken reagent to each test area of the slide. 5. Mix each test area with a disposable stirrer, spreading each mixture over the entire section. Use a clean disposable stirrer for each test area. 6. Rock the slide gently with a rotary motion for 2 minutes and observe immediately for agglutination.

After placing sample in this instrument, what are the names of two fractions in the tube (choose all that are correct)? ruer O Pellet OBase O Supernatant OFluid O Precipitation

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t /g = (Log Nt – Log N0) /0.301 10 mL of a culture of Enterobacter at 104 cells/mL is introduced into 990 mL of the same broth at the same temperature (37° C) with adequate mixing. It is 8 p.m. the night before class on Tuesday at 11 a.m. Doubling time for this species is 40 minutes. Will the culture reach Stationary Phase before class?

4. Answer the following questions about dilutions. b. C You take a culture of bacteria and dilute it 5 times. Each dilution uses 1 ml of culture and 9 ml of saline solution. You take.Iml from the most dilute tube and plate it on a TSA plate. The next day you count 61 colonies. What was the original concentration 6.1X10³ CFU/ ON 6.1X 10² CFU Od 61 X 10² CFU/ml 61X10 CFU/ You have a culture that has 5x10 cfu/ml of bacteria in it. You performa serial dilution by adding 1 mi of the stock in 9ml of TSB inTubeA and mix You then take 1 ml from Tube A and add it to 9 ml of TSB in Tube Band mix Last you take 1 ml from tube 8 and add it to a TSA plate. After overnight incubation, you count the colonies you see. How many colonies will you see? 5000 N 500 Od 50 Oas Oes You are doing a series of serial dilutions to find the concentration of bacteria in CFU/ml in a culture. You plate several of the dilutions tubes and count the colonies the next day. On plate A you find over 1000 colonies, plate B…

Complete the dilution series using a maximum amount of 20 mL of dilutent so that the dilution in the final tube is 10^-9

Hi, my question is 6 ml of urine is added to 30 ml of water. 0.1 ml is plated. 109 colonies form. What is the CFU/ml of the urine culture?