transferred to the plate. After 24 hours there were 60 colonies on the plate. What is the dilution factor here. What's the number of bacteria per ml of the original patient sample.   a. The dilution factor is 1000. The patient sample contains

A sterile powder vial label indicates to add 3.4 mL of sterile water which will produce a final volume of 4 mL. The increased volume is the result of: Select one: O a. O b. C. O d. The powder inside the vial was not lyophilized The type of diluent Displacement of the powder Incorrect calculation /mod/quiz/attempt.php?attempt=186101&cmid=102359&page=49# Q Search D L 贊助 8 9 Time left 22:22:18 HO

A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to determine the population of the culture, by transferring 25 ml of the suspension to 225 ml 0.90% NaCl. Three consecutive dilutions were further made by using I ml aliquot in 9 ml of 0.90% NaCl. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method. 田 Sampling COUNTS 1st dilution 54; 61 2nd dilution 3rd dilution 4h dilution 1st 3; 7 0; 0 0,0 2nd TNTC TNCT 242: 233 28: 37 3rd TNTC TNTC INTC 249-246 * TNTC = Too numerous to count i. Illustrate the dilution series used and label the final dilution of each dilution. ii. Determine the bacterial count (CFU/ml) every 4 hours of incubation for 12 hours. Show all computations.

1. You are asked to make 150 ml of 2% agarose using a well-known buffer. How many grams will be used? a) 0.03 g b) 0.3 g c) 3 g d) 30 g 2. Place the following reactants in their proper order for the indirect ELISA test 1 = enzyme-linked antibody 2 = known antigen 3 = patient serum 4 = substrate

A bacterial culture has a concentration of 3.2 x 108 cells /mL.  You dilute this culture as follows: 1/50, then 10-3 and finally 1/20.  If you then plate 0.2 mL of the final dilution, how many CFU would you expect following incubation?

You perform a serial dilution of a water sample and the 10-7 tube when 0.1 ml was plated onto the nutrient agar plates after incubation and gave you 24 colonies. What is the CFU/ml? What is a CFU?

Which of the following can be used to distinguish one colony from another on an agar plate?  Select all that apply. a. Shape b. Color c. Gram stain d. Cell arrangement e. Size f. Elevation g. Margin h. Texture

You make a smear of E. coli and then simple stain the slide with methylene blue. If you failed to use correct aseptic technique by not sterilizing the loop before picking up the bacterial sample for the smear, what results may be seen under the microscope?   A Both blue and purple cells may be seen on the slide. B No cells may be visible on the slide. C There may be cells of different shapes, sizes, and arrangements.

A stock solution of a bacterial strain is diluted 1/10000 to a total volume of 500ul. Then, 50ul of the dilution are spread onto a plate. The next day you count 150 colonies. What is the concentration of the stock solution in bacteria/ul?

the image contains results of the fluid thioglycolate medium tests. Please explain the results and the chemistry of each tube? Thank you