Dilution Factor 10,000 100,000 1,000,000 10,000,000 Plate colony count INTC* 613 120 18

Q: The solution containing bacterial spores is heated in an autoclave. When the autoclave has reached a…

A: Given that          Number of spores after autoclave reaches 121 C (No) = 106 spores/ml…

Q: ISOLATE #2 Results for "Isolate #2": ***Please fill out the table***. LBlood agar 2. Nitrute Test:…

A: Blood agar is a solid bacterial nutrient medium containing red blood cells generally from sheep.…

Q: You have added 8 mL of Albuterol Sulfate Solution (5mg/mL) and 22 mL of normal saline to your…

A: Introduction:-  Albuterol sulfate solution is in concentration of 5mg/ml and total of 8ml is to…

Q: Determine what percentage of the culture was living (viable) and what percentage was dead…

A: The word "CFU" in microbiology refers to a colony-forming unit, which is used to estimate how many…

Q: Make a flowchart for the procedure of SPREAD PLATE METHOD

A: The spread plate method is a method to plate a fluid sample containing bacteria with the goal that…

Q: . What is meant by a bacterial “colony” or colony-forming unit? 2. Why are colonies that develop on…

A: Since you've asked multiple questions, we're only answering the first three answers for you. If you…

Q: How would you prepare a 1:500 dilution? You cannot use more than 10mL per tube.

A: In scientific research and experimentation, it is often necessary to dilute substances to achieve…

Q: If there is a sample having 3.5x10^5 CFU/ml, and this cell count came from a sample of the original…

A: CFU stands for colony-forming unit that gives rise to a particular colony. It is generally assumed…

Q: In watermelons, bitter fruit (B) is dominant over sweet fruit (b), and yellow spots (S) are dominant…

A: Hi! Since you have posted a question with multiple sub-parts, we will solve the first sub-part for…

Q: In a plate count, 1 mL of culture is spread on a plate and incubated for 24 hours. 250 colonies are…

A: Given,number of colonies in a 1mL of culture = 250. Also,given that this given solution is 100 times…

Q: How to count the number of colonies forming units per milliliter (CFUs per ml) of the sample?

A: A viable cell is one that has the ability to divide and form a colony. The colony-forming unit (CFU)…

Q: transferred to the plate. After 24 hours there were 60 colonies on the plate. What is the dilution…

A: b. The dilution factor is 1000. The patient sample contains 600,000 cells/ml

Q: The predominance of squamous epithelial cells with few WBCs in a sputum sample would be adequate for…

A: The sputum culture is specific test that checks for bacteria or another type of organism that may be…

Q: For Staphylococcus aureus, indicate which tests need to be done & what media you need. Fill in box…

A: Staphylococcus aureus is a spherical-shaped cocci that belongs to the family of Firmicutes. It is…

Q: A sample of water was enumerated using a counting chamber (haemocytometer) and 220 bacterial cells…

A: The viable plate count, or simply plate count, is a count of viable or live cells. It is based on…

Q: For a laboratory exercise of antibiotic susceptibility test in a disk-diffusion method. What are the…

A: Antibiotics have definitely transformed the lives of countless individuals by rescuing them from…

Q: Describe how you would execute this first stage from the point you are handed the nutrient broth…

A: For isolation of any culture, it needs to be incubated in broth to obtain full fledged turbidity or…

Q: You are working in a medical lab and receive a urine sample from a patient that is displaying…

A:

Q: Answer the following questions based on Kirby Bauer / MacFarland standards 1-0.5 McFarland…

A: The Kirby Bauer test, also known as the disc diffusion test, is a common laboratory technique for…

Q: The PD# of your culture is 165. You plated 3745 cells per well. You have recovered 12,655 cells.…

A: The passage number of a cell culture is a record of the number of times the culture has been…

Q: What is the microbiology laboratory test that Identifies Pseudomonas masseli and what are the…

A: When doctors know what kind of germs are making someone sick, they can give the right medicine to…

Q: serial dilution

A: Dilution: It is defined as the process where the concentration of a solute in a solution is…

Q: discuss the significance of the different aseptic techniques utilized in maintaining sterility of…

A: The laboratory that carries out growth of prokaryotic cells or eukaryotic cells in physiological…

Q: The lag phase does NOT occur if an exponentially growing culture is transferred into a nutrient rich…

A: Microbial growth is said to occur when there is an increase in the number of cells rather than an…

Q: Drawings of the two bacterial cultures as observed under the 4 mm objective. Describe features…

A: Staphylococcus aureus and Streptococcus pyogenes are gram positive bacteria that cause infections in…

Q: The cell concentration measured in samples taken at certain intervals from a microorganism growth…

A: From the given table,   Cf, final cell concentration =0.376  Ci, initial cell concentration=0.01…

Q: Can you please discuss the principles of using Motility Test Medium for the detection of bacterial…

A: Bacterial motility is usually demonstrated in a semi-solid agar medium. Motile bacteria ‘swarm' in…

Q: A Pure colony is needed for inoculation in sterile water, a small portion of culture media was…

A: Introduction  Culture is referred to the process of growing the cell outside the animal or plant…

Q: What is the microbiology laboratory test that Identifies Pseudomonas putida and what are the results…

A: Pseudomonas putida is a species of gram negative bacteria belonging to the Pseudomonas genus. It is…

Q: Negative Control Unknown Isolate

A: Blood agar comes as a basal media and can be used as a general growth medium.  Blood agar is an…

Q: For an In vitro mimicking infection test:

A: Introduction: Cell culture is the process of growing cells in a controlled setting, usually outside…

Q: write a discussion in detail

A: By measuring the optical density (OD) of bacterial cultures at a wavelength of 600 nm, the growth…

Q: Calculate the CFU/mL of the original culture for the countable plate as shown in the diagram. b) How…

A: Introduction :- CFU( colony forming units ) are the count of cells present in orginal culture which…

Q: Remove the agar plate from its lid with the left hand. While holding the plate, inoculate the agar…

A: Introduction -- Microbes are around and every where in environment. Sterile agar plates which…

Q: If an OD=1.0 culture yielded a plate count of 2.2 x 107 cfu/mL. What is the cell count of an OD=0.5…

A: Answer: The relationship between colony-forming units per millilitre (cfu/mL) and optical density…

Q: You attempted to determine the number of bacteria in a culture using the Turbidimetry method with a…

A: Bacterial cell counts in a broth or culture can be determined by culturing multiple plates and…

Q: give the respective the TOTAL DILUTION FACTOR of respective serial dilutions. show complete…

A: Introduction: A serial dilution is any dilution in which the concentration decreases by the same…

Q: Provided with the following data, compute the corresponding CFU/ml of the original culture. ssume…

A: Ideally, plates with colony 25-250 colonies are used. As above 250 colonies are too numerous to…

Q: A patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution…

A: A sample of your blood, urine, skin, or any part of your body will be taken during a bacteria…

Q: From a water sample, you must determine the number of colonies present (CFU's/ml.). However, looking…

A: Counting with the CFU colony-forming units needs culturing the microbes. It counts only the viable…

Q: Colony of (bacteria): A cluster of cells (or clones) which arise from a single bacterium by asexual…

A:

Q: Joseph, being a know-it-all, told Mary that her method would not include all microbes. To…

A: Joseph and Mary had a disagreement about the effectiveness of Mary's method for counting microbes in…

Q: - What is a pure culture? - Describe the procedure for making a streak plate from a clinical…

A: Introduction A growth media, also known as a culture medium, is a solid, liquid, or semi-solid…

Q: Calculate the concentration of the overnight stock culture in CFU/mL

A: Colony-Forming Units (CFUs) refer to the number of individual colonies of any microorganism that…

Q: You perform a serial dilution of a water sample and the 10-7 tube when 0.1 ml was plated onto the…

A: Estimation of the number of viable cells is important in microbiology protocols. It is estimated…

Q: You are transferring a bacterial culture from a tube of broth to a Petri dish containing trypticase…

A: Bacterial culture: It is a technique that enables the controlled multiplication of bacterial cells…