This is the growth curve for Clostirdium (incubated under optimal growth conditions). Use it to answer the following questions. 3 Cell concentration (million cells/ml) N 0 0 2 The growth rate is lag log 4 0.2 0.25 0.32 0.5 units 6 million of cells per ml (Image description.: Horizontal axis is time in hours, Vertical is cell concentration in millions of cells per ml. Curve starts at 0,0, increases by 4 hours to 0.5 million cells/ml, goes up steeply over two hours from 0.5 million/ml at 4 hours to 2.5 million cells/ml at 8 hours, 8-12 hour shows an increase of 0.3 to 2,8 million cellls/ml and last 2 hours is flat at 2.8million cells/ml.) From 12 hr to 14 hr is called phase. lunar 8 Time (hours) 2 10 stationary 5 12 cells 14 death 16 million of cells per ml per hour hours million of cells

You wish to centrifuge and pellet yeast cells using a centrifuge. The protocol says that you needto centrifuge the culture at 2,000xg for 10 minutes at room temperature. The rotor of yourcentrifuge has a maximum diameter of 25.4cm and the minimum diameter of 12.4cm. At whatrpm should you spin the centrifuge for pelleting the cells?

You are determining the concentration of your culture, and have counted the following on your hemocytometer as shown in Figure below: (note, blue cells have taken up Trypan blue & thus are dead. Red x's are cells that should not be counted because touching one of 2 sides of square). If your cells were diluted 1:2 before counting, what is the concentration of cells in your original culture?

Below are morphological, physiological and biochemical characteristics of an unknown luminous bacterium isolated from the gills of a marine fish. Use the data below to identify the isolate. Make an identification scheme   Test Unknown Isolate B 1 Luminescence Positive 2 Cell Shape Straight Rods 3 Gram Staining Negative 4 Oxygen Requirement Facultative Anaerobe 5 Motility           (Hanging Drop) Motile 6 Growth at 0% NaCl Negative 7 3% NaCl Positive   8              6 % NaCl                                      Positive                   9 Accumulation of PHB Positive 10 Flagella 1 Polar 11 Oxidase Positive 12 Nitrate Reduction   13 Gelatinase Negative 14 Growth at 35OC Positive 15 Growth at 4OC Negative 16 Methyl Red Positive 17 Voges-Proskauer Positive 18 Catalase Positive 19 Starch Hydrolysis Negative 20 Lysine…