U have the plasmid pUC18/19, which is a circular plasmid that consists of 2686 bp. What would the number of and length of the fragments be if you cut the plasmid with the following restriction enzymes or combination of enzymes? Give a schematic representation of the digestions.PscI & GsuI______________________________________________________________ScaI, PdmI & BsaXI______________________________________________________________ScaI, SspI & EheI______________________________________________________________ Plasmiedkaart van pUC18/19 / Plasmid map of pUC18/19Eco01091 2674Pfol 46BstAPI 179Aatll 2617Ndel 183Sspl 2501,Ehel 235Pdml 2294,Bcgl 2215.Scal 2177146/236lacz2486MCS469BsaXI 659PUC18/192686 bpSapl 683852.AfllII, Pscl 806Gsul 1784.1626Cfr101 1779.Eco311 1766-Eam11051 16941466rep (pMB1)BseYl 1110\Cail 1217Ed13EIEco241SaclHinclEced Acc65Sral Kan399HirdllMISUC aqerong pirer (20, 17-na5' G TAA ANC GAC GGC CAG 1GC CAA GCT TGC ATG CCT GCA GGI CGA CTC TAG AGG ATC CCC GGG TAC CGA GCT CGA ATT CoT AAT CAI cer CAT AGC IGI 1IC CIG 3'PaelXbalBarHIXapl3 C ATT ITG CTG CGHOG GTT CGA ACG TẠC GGA CGT CCA GCT GAG ATC TCC TAG GGG CCC ATGCGA GCT TAA GCA ITA GTA CCA GIA TCG ACA AAG GAC 5Lacz - Val Val Ala Lou Ala Leu Ser Ala His Arg Cys Thr Ser Glu Leu Pro Asp Gly Pro Val Ser Ser Ser Asn Thr lle Met Thr Metbla (Ap)

A restriction endonuclease or restriction enzyme is a bacterial enzyme that cuts dsDNA into…

U have the plasmid pUC18/19, which is a circular plasmid that consists of 2686 bp. What would the number of and length of the fragments be if you cut the plasmid with the following restriction enzymes or combination of enzymes? Give a schematic representation of the digestions. PscI & GsuI ______________________________________________________________ ScaI, PdmI & BsaXI ______________________________________________________________ ScaI, SspI & EheI ______________________________________________________________

U have the plasmid pUC18/19, which is a circular plasmid that consists of 2686 bp. What would the number of and length of the fragments be if you cut the plasmid with the following restriction enzymes or combination of enzymes? Give a schematic representation of the digestions. PscI & GsuI ScaI, PdmI & BsaXI ScaI, SspI & EheI __

Essentials of Pharmacology for Health Professions

7th Edition

ISBN:9781305441620

Author:WOODROW

Publisher:WOODROW

Chapter5: Abbreviations And Systems Of Measurement

Section: Chapter Questions

Problem 5CRQ

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