Write down the primers spesific to Theileria annulata enolase. Calculate the Tm for each primer and calculate the annealing temperature for your PCR (use pUC19 vector for the design). Sall HincII EcoRI Крп1 ВатHI Аccll PstI HindIII SacI Xmal Xbal BspMI SphI Smal Polylinker lacZ* 2.686/0 kb ori PUC19 (2.686 kb) ampR. ori = Origin of replication sequence ampR = Ampicillin resistance gene lacZ* = B-galactosidase gene

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Write down the primers spesific to Theileria annulata enolase. Calculate the Tm for each primer
and calculate the annealing temperature for your PCR (use pUC19 vector for the design).
Sall
HincII
EcoRI
Крп1 ВатHI Аccll
PstI
HindIII
SacI
Xmal
Xbal BspMI SphI
Smal
Polylinker
lacZ*
2.686/0 kb
ori
PUC19
(2.686 kb)
ampR.
ori = Origin of replication sequence
ampR = Ampicillin resistance gene
lacZ* = B-galactosidase gene
Transcribed Image Text:Write down the primers spesific to Theileria annulata enolase. Calculate the Tm for each primer and calculate the annealing temperature for your PCR (use pUC19 vector for the design). Sall HincII EcoRI Крп1 ВатHI Аccll PstI HindIII SacI Xmal Xbal BspMI SphI Smal Polylinker lacZ* 2.686/0 kb ori PUC19 (2.686 kb) ampR. ori = Origin of replication sequence ampR = Ampicillin resistance gene lacZ* = B-galactosidase gene
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