Procedure Restriction maps for Lambda DNA Lambda DNA may exist as a linear or circular molecule. The linear Lambda DNA is 48,502 base pairs in length. It contains five EcoR I restriction sites or cleavage sites. These restriction sites are illustrated below. Each number indicates the distance, in base pairs, that the restriction site is located from the end of the linear Lambda DNA molecule. Hind III recognizes seven restriction sites. BamH I recognizes five sites. Figures 1-3 show the restriction sites for the enzymes we will use. In the spaces provided below each map, calculate the size, in base pairs, of the individual fragments produced by each enzyme. 26,104 21,226 31,747 39,168 44,972 48,502 Number of I DNA fragments produced by digestion with EcoR I: Size, in base pairs (bp), of fragments produced by EcoR I digestion: Figure 1: Restriction sites for EcoRI 27,479 37,584 23,130 36,895 44,141 25,157 37,459 48,502 Number of fragments produced: Size of fragments produced: Figure 2: Restriction sites for Hind II 27,947 5,505 22,346 34,499 41,732 48,502 Number of fragments produced: Size of fragments produced: Figure 3: Restriction sites for BamH I

How much 340bp insert DNA should be added to a ligation in which 50ng of 4.3kb vector will be used? The desired vector: insert molar ratio needs to be 1:3.

Can you please help with 1c please  picture with 1 graph is for question 1a) picture with 4 graphs is for question 1b) 1a) E. coli DNA and binturong DNA are both 50% G-C. If you randomly shear E. coli DNA into 1000 bp fragments and put it through density gradient equilibrium centrifugation, you will find that all the DNA bands at the same place in the gradient, and if you graph the distribution of DNA fragments in the gradient you will get a single peak (see below). If you perform the same experiment with binturong DNA, you will find that a small fraction of the DNA fragments band separately in the gradient (at a different density) and give rise to a small "satellite" peak on a graph of the distribution of DNA fragments in the gradient (see below). Why do these two DNA samples give different results, when they're both 50% G-C? 1b) If you denatured the random 1000 bp fragments of binturong DNA that you produced in question 1a by heating them to 95ºC, and then cooled them down to 60ºC…

Even though the eukaryotic genome is thousands of times larger than the prokaryotic genome, DNA replication times are relatively similar. Explain how this is possible.

1) Prepare the following enzymatic reaction, present it in tabulated form. In a final volume of 30 ul, where buffer 4 (10 ml). How much volume of each reagent would be used and how much of water? Is there any problem? 2) The DNA pol 1 enzyme comes at a concentration of 50,000 U/ml. You have to prepare a 50 ug PCR reaction where you must use 0.05 U/ml reaction. You add 10 ul of PCR buffer, 2 ng of tempered DNA that is at a concentration of 0.5 ng/ul, primers (which are at 200 mM) so that each one remains at a concentration of 200 uM, Mg+2 that is 5 mM (10 X), enzyme and water. Present the table of all the reagents included in the reaction, the volumes of each one in ul. Present where the initial and final concentration of each reagent applies. Assume you have micropipettes for all values.

Below is an EMSA showing four different reactions, A-D. In each tube there is some combination of labelled DNA probe, Protein X (the protein you are studying), and an antibody for Protein X. Identify which combination of components are found in each of the four reactions and explain how you determined that based on the molecular interactions being studied and your knowledge of gel electrophoresis. It is possible that multiple lanes have the same component(s). A B C D EMSA

How would I calculate sanger sequencing fragments that will produce green

All ingredients required for the synthesis of DNA were placed in a test tube.  The primer has the sequence 5’-TCGATCA-3’. First determine where the primer will bind to the DNA template given below (Do this by writing the sequence of the primer on top of the template – make sure you find a location that is complementary in sequence and that the primer is antiparallel with respect to the template) and then indicate the nucleotides that DNA polymerase would attach onto the primer as it synthesizes DNA (hint: in which direction does DNA polymerase attach nucleotides onto a primer?)  See figure 13.16 in book.   5’-GACGTAGTCTGATGCTAGCATGCTGATCGAAAGAG-3’

a. Deoxyribonucleic acid (DNA) is a molecule composed of two polynucleotide chains that coil around each other to form a double helix carrying genetic instructions for the development, functioning, growth and reproduction of all known organisms and many viruses. Select from the drop down menus to complete the complimentary strand for the following DNA molecule: A [ Select ] [ Select ] G [ Select ] [ Select ] [ Select ] A [ Select ] [ Select ] [ Select ] [ Select ] > > > > > >

You digest 4 uL of plasmid DNA that is 50 ng/uL concetration in a total volume of 20 uL. You run 10 uL of the digest on teh gel. You then do a DNA purification protocol with a Zippy prep on the remaining digested DNA. You elute the DNA in a 25 uL. A 2 uL ssample has the concentration of 2 ng/uL. What is the DNA yield?