True or False: Restriction enzymes normally prevent viral DNA or RNA entry in bacteria.

Q: In the given below option which of the following are considered as the main tools for DNA…

A: DNA technology alters the phenotype of the organism through a genetically altered vector.  It…

Q: The genome of bacteria cells, eukaryotic cells, and viruses are all about the same size. True or…

A: Genomes can be described as the haploid set of chromosomes present in an organism.

Q: Compare the process of polymerase chain reaction (PCR) to DNA replication.  Discuss three…

A: PCR is in vitro process whereas DNA replication is in vivo process.

Q: The Human Genome Project attempts to modify organism's DNA inserting desired genes into bacteria.…

A: The gene is known as the unit of heredity, and this unit is both physical and functional as these…

Q: How is bacterial DNA safe from digestion by Restriction Endonucleases?     The plasmid of the…

A: Question-  How is bacterial DNA safe from digestion by Restriction Endonucleases?   A) The…

Q: Basically the Ames Test is a procedure that analyzes the mutagenic ability of Salmonella…

A: A scientist “Bruce Ames” devised the Ames test in 1970s which is used to analyze the carcinogenic…

Q: An insertion of two nucleotides into a gene results in a frameshift mutation.  This statement is:…

A: The nucleotide sequences found within a DNA molecule are subject to change due to a phenomenon known…

Q: TRUE OR FALSE: Mupirocin inhibits bacterial cell wall synthesis. Mupirocin is used as a negative…

A: we have to answer wether the given statement is true or false . Due to question answering policies…

Q: Statement 1: Restriction enzymes are sequence-specific, which means that they only recognize a short…

A: Restriction endonuclease is an important tool in DNA Recombinant technology or genetic engineering.…

Q: The strand of DNA that is replicated continuously in the 5' to 3' direction is referred to as:…

A: DNA replication: DNA replication is a fundamental biological process in which a cell makes an exact…

Q: The enzymes mentioned below are used as tools during cloning, DNA sequencing and/or gene therapy.…

A: Enzymes  Enzymes are the protein that use as catalysts in metabolic and catabolic reaction to fasten…

Q: Which of the options listed below can serve to carry a gene from one organism into a bacteria cell?…

A: The specific genes or pieces of DNA are transported or carried from one organism to another. These…

Q: In order to transform a bacteria, the cell wall should be perforated either through physical or…

A: The cell wall is the very outer membrane of the cell. It has a rigid, semi-permeable protective…

Q: An viral enzyme that can be used to create complementary cDNA is DNA polymerase. reverse…

A: The genome of an organism is defined as the whole heredity information encoded in the genetic…

Q: You culture bacteria from the soil at a toxic waste dump on an agar plate and pick a single…

A: In this scenario you have isolated a previously uncharacterized species of bacteria from a soil…

Q: Restriction digestion of DNA fragments is not sequence specific. True False

A: Within a few years after discovering EcoB, EcoK, and HindII, scientists were already experimenting…

Q: Base oxidation (small damage to a single nucleotide) is repaired using:

A: Introduction:DNA damage can occur due to various factors, including exposure to reactive oxygen…

Q: process inside a cell. Therefore, the following essential components: DNA polymerase DNA template…

A: PCR stands for Polymerase chain reaction in which a given stretch of DNA is amplified into millions…

Q: Conjugation is the uptake of DNA from the environment.   true or false

A: The correct response to this particular question will be, False

Q: a restriction enzyme is one that?

A: A restriction enzyme is a most important tool in recombinant technology. The alteration of the…

Q: Which of these statements is INCORRECT about restriction enzymes

A: A restriction enzyme is a protein isolated from bacteria. It help to  cleaves DNA sequences at…

Q: Which repair mechanism repairs longer stretches of damage:   Question 22 options:   base excision…

A: Nucleotide excision repair (NER) is a DNA repair mechanism that corrects certain types of DNA damage…

Q: Which of the following protocols is BEST used for introducing exogenous DNA into a cell with a thick…

A: The gene gun approach is the most effective method for transferring exogenous DNA into a cell with a…

Q: Explain each section of the flowchart in detail by integrating all the concepts. The explanation…

A: The process by which identical copies of DNA (deoxyribonucleic acid) or fragments of DNA are…

Q: The genomic DNA of a bacterial cell is not destroyed by the cell’s own restriction enzymes because…

A: BASIC INFORMATION  RESTRICTION ENZYME  they are also known as molecular scissors it was in the year…

Q: How do the following factors affect the activity of restriction enzymes? pH conditions Mg2+…

A: Restriction enzymes (restriction endonucleases) are the enzymes that can cleave at specific sites in…

Q: Reverse transcriptases are RNA-dependent DNA polymerases that use deoxyribonucleotides as…

A: Reverse transcriptase is the enzyme which is used by viruses such as HIV for the purpose to…

Q: Bacteria are the most common GMOs because their simple structure permits easy manipulation of their…

A: Recombinant DNA is a technique for cutting and pasting DNA sequences of interest using enzymes.

Q: A DNA fingerprint consists of sections of DNA that have been cut with restriction endonuclease…

A: DNA fingerprinting DNA fingerprinting is a modern technique that help us to solve  crimes. The…

Q: An 800 bp fragment is completely digested with a single restriction enzyme. Fragments of 50*, 150*,…

A: Restriction enzymes are those enzymes which recognize and cleave at a particular sequence. In this…

Q: If this gene is methylated, which of the following combinations of DNA fragments would you expect…

A: Answer: RESTRICTION ENZYME = It is the enzyme which cuts the strands of DNA at specific sites…

Q: Some restriction enzymes produce DNA fragments with overhanging stretches called sticky ends on each…

A: The enzymes which are used to cut the DNA strands are called restriction enzymes. They are also…

Q: Tailed primer can have restriction enzyme sites. True False

A:

Q: State the function of each chemical/components below in DNA extraction    Salt:  Detergent:…

A: Breaking cells open to release the DNA.  Separating DNA from proteins and other cellular debris.…

Q: Describe what will happen to bacteria with dysfunctional restriction modification system providing…

A: Bacteria is a microorganism that has many defense mechanism to prevent the infections from the other…

Q: In a conventional PCR reaction cycle, which of the following step is NOT needed for a successful PCR…

A: Polymerase chain reaction (PCR) is a scientific method that exponentially multiplies a single copy…

Q: Imagine that you are a student in Alfred Hershey and Martha Chase’s lab in the late 1940s. You are…

A: Alfred Hershey and Martha Chase’s experiment result that the Dna is the genetic material and the…

Q: A probe is a full section of DNA or RNA that has been labeled with a tracer.  True  False

A: DNA or RNA probe are nothing but the stretches of single stranded DNA or RNA which are labelled with…

Q: Although CRISPR uses some of the same viral vectors as "gene therapy," which was pioneered in the…

A: Gene therapy is the process of changing the genes in the body's cells in order to treat or prevent…

Viral Replication

The virus is acellular and considered non-living outside the host cell. The replication of the virus occurs only inside the living cells. Only a few genes are present in the smallest virus, whereas many genes are present in the largest virus. These genes encode specific viral proteins.