True or False : CRISPR is a novel method for reliably amplifying gene products so that they can be sequenced and studied.

Both microarray and RNA-sequencing can study the transcriptomes. Compare microarray and RNA sequencing technique in analyzing gene with high, medium and low copy number of genes.

List the components that you would need in order to use the CRISPR-Cas9 system for gene editing

After running a qPCR experiment, we will have graphs showing the amount of fluorescence detected by the digital camera compared to the number of PCR cycles run. Suppose you see the following graph output by the qPCR machine: Relative Fluoresence 3.0 2.5 2.0 1.5 1.0 0.5 0.0 0 10 20 30 40 50 Cycles Which curve (blue, red, or green) represents a sample with the smallest amount of mRNA present? Why? Be sure to discuss Ct values in your answer.

A number of advances have been made in biotechnology. CRISPR/Cas9 one of the most controversial, and has had a lot of media attention in recent years. It is a method by which scientists can precisely edit DNA sequences at exact locations. Benefits obviously include the potential to “repair” mutated genes that cause disease. In fact, preliminary results from one of the earliest clinical trials of CRISPR/Cas9 provide evidence that the technique is safe and feasible to use for treating human diseases. What other potential applications of this application do you see (you can use any organisms to illustrate your answer)? What are the potential dangers or downsides of using this technology? Do you think this technology should be used in gene editing in humans? Explain your stance.

Some people have argued that editing the genomes of human embryos is ethically defensible as long as the embryos are not allowed to develop past an early stage and thus will never result in the birth of genetically modified humans. Others have argued that no genome editing should be carried out on human embryos. Present arguments for and against using genome editing on human embryos.

write the pros and cons of crispr technology in bullet points with explaintion

A patient is diagnosed with lung cancer using imaging. To establish the best treatment option, doctors ask to profile the gene expression of the tumor, using a biopsy and microarray analysis. Describe the steps involved in the analysis in detail. The analysis reveals that a new gene therapy could be beneficial to the patient. Explain briefly how gene therapy could be used to cure cancer and detail how the treatment can be formulated for delivery to the patient. To prepare DNA for the gene therapy formulation, it is often important to measure the size of the molecule. Explain how this measurement can be done using agarose gel electrophoresis, taking care to detail the property of the DNA molecule at the basis of the technique. To generate large amounts of DNA to manufacture gene therapy payloads or to be able to see them on gel electrophoresis, specific sequences can be amplified by PCR. For a sequence of a 100 base pairs, calculate the number cycles of PCR required to generate 1 ng of…

CRISPR techniques allow scientists to modify specific genes while sparing all others, thus clarifying the association between a given gene and its consequence to the organism. How can this help to create better antibiotics or to make white blood cells resistant to HIV infection? Give your examples. What is the future of CRISPR?

What is, The ethical implications of gene editing in humans. 1. Introduction: a) Begin with a captivating opening that introduces the topic and its significance. b) Clearly state the thesis statement in this case 2. Body: a) Start with the first main point that discusses the potential benefits of gene editing in humans. b) Transition to the second main point which focuses on the ethical concerns and risks associated with gene editing in humans. Discuss issues like equity fairness and the potential for unintended consequences such as harmful side effects or genetic discrimination. c) Present counterarguments to your points. Acknowledge opposing viewpoints but refute with logical reasoning and supporting evidence emphasizing the importance of considering potential long-term consequences and the need for strict ethical guidelines and regulations. 3. Conclusion: a) Summarize your main points and restate your thesis statement emphasizing the ethical implications of gene editing in…