If this gene is methylated, which of the following combinations of DNA fragments would you expect from this restriction digest?

Human Anatomy & Physiology (11th Edition)
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Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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Dr. Doom has a DNA sequence of 2000 bp.
Enzyme X and Enzyme Y restriction sites and their positions are indicated here. Recall that
Enzyme X is methylation sensitive, while Enzyme Y is not.
200
O 100
1800
2000 bp
1000
1500
Enzyme Y
Enzyme Y
Enzyme X
Enzyme Y
Enzyme X
If this gene is methylated, which of the following combinations of DNA fragments would
you expect from this restriction digest?
O 5 bands = 1O bp, 200 bp, 1000 bp. 1500 bp, and 1800 bp
O 3 bands = 200 bp, 500 bp, and 1300 bp
%3D
O No bands = DNA will be completely digested
%3D
O 4 bands = 100 bp. 200 bp, 800 bp, and 900 bp
Transcribed Image Text:Dr. Doom has a DNA sequence of 2000 bp. Enzyme X and Enzyme Y restriction sites and their positions are indicated here. Recall that Enzyme X is methylation sensitive, while Enzyme Y is not. 200 O 100 1800 2000 bp 1000 1500 Enzyme Y Enzyme Y Enzyme X Enzyme Y Enzyme X If this gene is methylated, which of the following combinations of DNA fragments would you expect from this restriction digest? O 5 bands = 1O bp, 200 bp, 1000 bp. 1500 bp, and 1800 bp O 3 bands = 200 bp, 500 bp, and 1300 bp %3D O No bands = DNA will be completely digested %3D O 4 bands = 100 bp. 200 bp, 800 bp, and 900 bp
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