The crystal structure has been determined for the complete 12-subunit yeast RNA polymerase II bound to a transcription bubble and product RNA. Yes or no

In Figure 12-26, provide a biochemical mechanism forwhy HP-1 can bind to the DNA only on the left side of thebarrier insulator. Similarly, why can HMTase bind onlyto the DNA on the left of the barrier insulator?

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In bacteria, a transcription experiment in vitro with purified RNA polymerase holoenzyme is fairly straightforward. In eukaryotes, it is not. Explain this observation, including what would be meant by the term “holoenzyme” for a eukaryotic RNA polymerase.

Mcm2 is a protein of the helicase complex that recognizes origin in Eukaryotes. But it is also related to cancer progression in humans. Please describe the role of Mcm2 in cancers and the potential uses of its inhibition in cancer treatment.

18 Actin can be used as a loading control in a western blotting experiment. What is the purpose of a loading control?

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What are two possible anticodon sequences for tRNA's that are charged with Phenylalanine (Phe)? O 3' AAU 5' and 3' GAA 5' O 3' AAA 5' and 3' AAG 5 3' UUG 5' and 3' CUG 5' O 3' GAA 5' and 3' CAG 5' 3' GUA 5' and 3' GUG 5'

Table 1 shows a list of restriction endonucleases with their recognition sequence and the sites of cleavage indicated by arrows. Table 1 Enzyme name Recognition sequence and position of cut 5'GIAATTC3 5'G!GATCC3' 5'GIGTACC3 5'GCIGGCCGC3' 5'IGATC3' 5'GGTACIC3' 5'ALGATCT3 EcoRI ВатHI Аcс651 Notl Sau3A Kpnl BglII (i) Which restriction enzyme(s) produce blunt ends? (ii) Are there any pair of neoschizomers in the list? Explain. (iii) Are there any pair of isocaudomers in the list? Explain.

You have recently cloned multiple cDNAs for a gene you are studying. After sequencing ten of the cDNAs, you find that seven of the cDNAs would produce a protein of 531 amino acids, while the other three cDNAs would produce a protein missing 30 amino acids from the center of the protein. This finding is can be explained by: O nonsense suppression O alternative splicing O basepair wobble O variability in the length of the poly(A) tail