The two factors that bind directly to the DNA at Pacific sequences are TFIID and TFIIB- in general terms what is the function of each of these two transcription factors in the initiation of transcription

Molecular biology, please explain in detail

11c all three of the classes or types of eukaryotic transcriptional regulators have alpha helical structure. name any two of the three major classes of eukaryotic transcriptional regulators.

47.

Three overall methods of controlling EUkaryotic transcription are (check the correct 3 of these. Don't check more than 3.) using transcription factors denaturing proteins using operons DNA phosphorylation O DNA methylation chromatin remodeling controlling RNA stability O proofreading O performing ectopic recombination

1. Please tell me: What's a mutation in moleculer terms? 2. When a mutation deletes a base in genomic DNA, how does it affect the expression product production and reading frame?  3. How does induction control enzyme synthesis using lactose operon?

6

1. enzymes that catalyze histone acetlation are closesly associated with transription factors, which are proteins that promote transcription. why is this a good biochemical strategy? 2. sp1 is a sqeuence specific human DNA binding protein that binds to a region on the DNA called the GC box, a promoter element with the sequence GGGCGG. Binding of Sp1 to the GC box enhances RNA polymerase 2 activity 50- to a 100 fold. How would you use affinity chromatography to purify SP1?