You are studying the regulation of a bacterial gene (TolU) that encodes an enzyme that is necessary for the bacterium to degrade toluene for use as a carbon source. The tolU gene is only transcribed when simple sugars are not available as a carbon source. You isolate three mutant strains of this bacterium, each of which harbors a single mutation: tolA-, or tolB-, or tolC-. To understand the nature of this defect, you also create partial-diploids in which the mutant strain has a F' factor with different genes as shown in the table below in bold. TolA, TolB, and TolC are all regulatory components (either promoter, or operator, or repressor) involved in TolU regulation. The expression of the repressor protein is not under the control of the TolU promoter. The table below are the phenotypes of different strains that you have constructed. (NOTE: + indicates wild type and indicates mutated) Activity of TolU Simple Genotype Simple sugars sugars absent present 1 A+ B+ C+ U+ A- B+ C+ U+ A+ B- C+ U+ A+ B+ C- U+ A+ B+ C- U+/ F' A+ B+ C+ U+ A-B+ C+ U+/ F'A+ B- C+ U+ A+ B+ C-U-/ F' A+ B+ C+ U+ A- B+ C+ U+/ F' A+ B+ C+ U+ + 6. 7 8 a. Is tol A the promoter, repressor, or operator? a. Is tol B the promoter, repressor, or operator? a. Is tol C the promoter, repressor, or operator?
You are studying the regulation of a bacterial gene (TolU) that encodes an enzyme that is necessary for the bacterium to degrade toluene for use as a carbon source. The tolU gene is only transcribed when simple sugars are not available as a carbon source. You isolate three mutant strains of this bacterium, each of which harbors a single mutation: tolA-, or tolB-, or tolC-. To understand the nature of this defect, you also create partial-diploids in which the mutant strain has a F' factor with different genes as shown in the table below in bold. TolA, TolB, and TolC are all regulatory components (either promoter, or operator, or repressor) involved in TolU regulation. The expression of the repressor protein is not under the control of the TolU promoter. The table below are the phenotypes of different strains that you have constructed. (NOTE: + indicates wild type and indicates mutated) Activity of TolU Simple Genotype Simple sugars sugars absent present 1 A+ B+ C+ U+ A- B+ C+ U+ A+ B- C+ U+ A+ B+ C- U+ A+ B+ C- U+/ F' A+ B+ C+ U+ A-B+ C+ U+/ F'A+ B- C+ U+ A+ B+ C-U-/ F' A+ B+ C+ U+ A- B+ C+ U+/ F' A+ B+ C+ U+ + 6. 7 8 a. Is tol A the promoter, repressor, or operator? a. Is tol B the promoter, repressor, or operator? a. Is tol C the promoter, repressor, or operator?
Human Anatomy & Physiology (11th Edition)
11th Edition
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Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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Transcribed Image Text:You are studying the regulation of a bacterial gene (TolU) that encodes an enzyme that is
necessary for the bacterium to degrade toluene for use as a carbon source.
The tolU gene is only transcribed when simple sugars are not available as a carbon
source.
You isolate three mutant strains of this bacterium, each of which harbors a single
mutation: tolA-, or tolB-, or tolC-.
To understand the nature of this defect, you also create partial-diploids in which the
mutant strain has a F' factor with different genes as shown in the table below in bold.
TolA, TolB, and TolC are all regulatory components (either promoter, or operator,
or repressor) involved in TolU regulation.
The expression of the repressor protein is not under the control of the TolU promoter.
The table below are the phenotypes of different strains that you have
constructed.
(NOTE: + indicates wild type and indicates mutated)
-
Activity of TolU
Genotype
Simple
Simple
sugars
sugars
absent
present
1
A+ B+ C+ U+
A- B+ C+ U+
A+ B- C+ U+
A+ B+ C- U+
A+ B+ C- U+/F' A+ B+ C+ U+
A-B+ C+ U+/ F' A+ B- C+ U+
+
7
A+ B+ C-U-/ F' A+ B+ C+ U+
+
8
A- B+ C+ U+/ F' A+ B+ C+ U+
a. Is tol A the promoter, repressor, or operator?
a. Is tol B the promoter, repressor, or operator?
a. Is tol C the promoter, repressor, or operator?
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