Repeated freezing and thawing, sonication, French press, etc. are some of the methods for enzyme extractions. Explain the reason for performing these steps and the type of enzymes that require to go through these steps.
Repeated freezing and thawing, sonication, French press, etc. are some of the methods for enzyme extractions. Explain the reason for performing these steps and the type of enzymes that require to go through these steps.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:Repeated freezing and thawing, sonication, French press, etc. are some of the methods
for enzyme extractions. Explain the reason for performing these steps and the type of
enzymes that require to go through these steps.
Expert Solution
Step 1reason for perfoming these steps
These steps are performed for cell disruption to release the desired biomolecules from cells. This cell disruption technique
divided into 2 types depending on the type of tissues are as follows:
The first one is Gentle disruption includes freeze-thaw lysis, enzymatic lysis, detergent lysis, and osmotic lysis.
second is Harsh disruption includes mechanical homogenization, French press, sonication, and grinding.
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