If a technician examined a Kirby-Bauer assay and found individualcolonies of the plated microbe growing within the zone of inhibitionfor a drug, what should her conclusion be?
Q: (a) Which would be easier to prepare bacterial smear from, liquid or solid culture? Why? (b) Aside…
A: Introduction A bacterial smear is a small layer of bacteria that has been stained on a slide. A…
Q: Why does the oxidase test have to be read quickly?
A: The Oxidase test is a biochemical test for the identification of bacteria. The test is mainly…
Q: You aseptically transfer 1 mL of your original liquid culture into 99 mL of sterile water. What is…
A: The dilution factor is a term used to describe the ratio of the final volume over the aliquot…
Q: The gelatin medium required inoculation with 3 loopfuls of culture. Why was so much organism…
A: Gelatin hydrolysis test is carried for detecting the ability of the bacteria to produce protease…
Q: How could you utilize fluorescence quenching in the different analytical application? Please answer…
A: Fluorescence quenching is a significant method for estimating restricting partiality among ligands…
Q: Why are biochemical tests used to identify microbes?
A: Microbes are living organisms that are very small and can be seen only with the help of a…
Q: To decrease the unwanted biological damage for the patients during an x-ray test, we can: *
A: In laser production, increasing the density of the lasing medium will increase the number of photons…
Q: Why should the use of nichrome or iron containing loop/wire be avoided as an inoculating device when…
A: The oxidase is a test used in microbiology to identify bacteria that produce cytochrome c oxidase.…
Q: What are the advantages of the API 20E test compared to regular biochemical tube media
A: API 20E test helps in the determination of bacteria from lab-scale activated sludge treatment…
Q: How to do cellulolytic characterization using a 96-well plate? What is the protocol or procedure to…
A: Cellulolytic action can be measured by an assortment of techniques that have been summed up in…
Q: identify the organisms in an unknown culture. You find that the culture contains two gramnegative…
A: A culture is a medium to grow bacteria and other microorganisms It provides the bacteria with…
Q: Practical techniques used to identify unknown microorganisms’ You should include the types of tests…
A: Microbiology is the study of microorganisms, identification, and classification of a particular…
Q: After finishing running IEX on HPLC, you found out that the detector on HPLC was not on during…
A: An extracted-ion chromatogram is generated by separating the ions of interest from a data file…
Q: Design an experiment to determine whether an antimicrobial agent is acting as a cidal or static…
A: A cidal agent means that it causes death of an organism. A static agent does not directly kill the…
Q: What side of the electrophoresis chamber (red or black) should the wells be on when the gel is…
A: The agarose gel electrophoresis is a technique that is used to separate DNA fragments as per their…
Q: What is an E-test strip, and what is it used for in the microbiology laboratory? Draw a diagram of…
A: Microbiology is a branch of science which deals with the study of microscopic organisms such as…
Q: In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable…
A: Harada-Mori culture technique It is a method of incubating fecal material on a filter paper strip in…
Q: Discuss the medical application of the Benedict’s test? What other test(s) are used in parallel to…
A: Benedict's test is used to test for simple carbohydrates. Benedict's test identifies reducing…
Q: Describe the Ninhydrin test. What does Ninhydrin test detect?
A: The ninhydrin test is a chemical procedure for detecting ammonia, primary/secondary amines, and…
Q: State the principle that underlies the following biochemical tests: a) Methyl red test b)…
A: Please note that as per the guidelines of Bartleby, only question no. 1 with both subparts (a) and…
Q: Discuss the significance of the following processes and what constituent is being determined: a.…
A: Borntrager’s Test: This is a quantitative chemical test for the glycosides in any given sample, this…
Q: Complete the following table summarising the neutral red assay data from the group following…
A: Cell Viability Assays -- It is a measure of the proportion of healthy , live cells with in a…
Q: which method do you think is more reilable, a test utilizing microbial growth media, or a…
A: The investigation of microorganisms is significantly aided by the ability to culture them, that is,…
Q: Which technique is preferred in the separation of fatty acyl methyl esters from a mixture?a)…
A: Chromatography is defined as the physical method that can be used for separating different…
Q: Voges Proskauer test Does color of the medium change to red after both reagents were added and…
A: Biochemical method of bacterial identification is a classical method that is used for the…
Q: Please discuss how the Methyl Red-Vogues Proskauer test helps distinguish microbes from each other.
A:
Q: How could hemophiliacs benefit from affinity affinity chromatography?
A: Hemophilia shows the X linked recessive pattern of inheritance.This disease mostly affected the…
Q: How can UV radiation, Gamma radiation, and Filtration of controlling microbial growth affect cells?
A: Several physical methods are used to control the growth of microorganisms. Radiation and filtration…
Q: Before the experiment was run, the apparatus was sterilized and then carefully sealed. Why was this…
A: Sterilization can be classified into physical and chemical methods. Physical methods include the use…
Q: Outline in detail the procedure for celloidin embedding .
A: Embedding is that the method during which the tissues or the specimens are capsulate in a very mass…
Q: Your labmate forgot to use CBB-R250 in the process. Which of the following would most likely be the…
A: The Coomassie brilliant blue is a staining dye used in the laboratory to quantify, visualize the…
Q: What could be possible errors when you incorrectly identify an Unknown organism when using IMViC…
A: IMViC tests are used to identify bacteria from specimens collected from patients in majority of…
Q: State the principle that underlies the following biochemical tests: a) Methyl red test
A: Fermentation is an anaerobic process where glucose or other carbohydrates are metabolized to produce…
Q: Which of the following is a reason to run simultaneous tests on known positive and negative controls…
A: A negative control is a control group in an experiment that uses a treatment that will not produce…
Q: What is a step-by-step process of preparing and inspecting a microorganism under a microscope?…
A: In dark-field microscopy, microbes appear light in a dark background., viruses, macromolecules,…
Q: Explain with the examples of biochemical test that are used to identify the bacteria in the…
A: The smallest organism that is usually defined that the are been can be seen with the naked eye is…
Q: list the reagents used in the IMViC tests and what they detect
A: IMViC is a series of tests that include the indole test, methyl red test, Voges-Proskauer test, and…
Q: What are the advantanges and disadvantages of using stained preparations for observing microbial…
A: Characteristics of microbes are studied using a microscope by microbiologists. Some microbes like…
Q: Briefly, in your own words, describe the steps of the gram staining procedure.
A: The bacterial kingdom is classified into Gram positive and Gram negative bacteria depending on their…
Q: Which of the following is a reason to run simultaneous tests on known positive and negative controls…
A: Biochemical test are different types of test carried out to find the nature and identify unknown…
Q: What is the basic purpose of the paper chromatography?
A: Chromatography is an important analytical method used to separate a mixture of chemical substances…
Q: escribe the examples of biochemical test that are used to identify the bacteria in the laboratory.
A: According to the definition of a biochemical test, it is a test that is used to distinguish between…
Q: You have retrieved your MHA (Mueller-Hinton agar) plate, your materials, and your E. coli culture.…
A: Answer : First step of the kirby-Bauer test :-
Q: How is fluorescence spectroscopy beneficial for pharmacological analysis ? Please shortly answer at…
A: Fluorescence spectroscopy uses a beam of light that excites the electrons that are present in the…
Q: Suppose your Professor handed you a test tube with 2.0mL of E.coli broth culture and told you to…
A: In both basic and applied research, "microbiology" is a highly significant area. Proteomics,…
If a technician examined a Kirby-Bauer assay and found individual
colonies of the plated microbe growing within the zone of inhibition
for a drug, what should her conclusion be?
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- The following pictures show the results of a Disk Diffusion Assay for different types of bacteria. For each bacteria, what antibiotic would you recommend be used on the patient? Explain your choice.3) Were all of the conditions of a standardized Kirby-Bauer test met as you performed this assay? If not, which were not? 4) What is the significance of colonies that develop within otherwise clear zones of inhibition? If the laboratory report for one of your patients indicated colonies within the zone, what concerns would you have for your patient?Construct a schematic diagram on how ACE inhibition assay is performed based on the protocol given. Explain how the colorimetric method measures ACE inhibitory activity. Protoc reference:
- The images attached are the photos of bacteria in yoghurt under a misroscope. According to these images and your own knowledge, can you make a biological drawing of bacteria in yoghurt ( in a circle) and identify the types of bacteria accurately?If no amplified product was visualized by UV light on the gel, does it mean that there is no water-borne microbial pathogen in the unknown sample?When viewed under a microscope, bacteria appear as red rods after they have been stained. Explain a quick process for determining the outcome of this experiment.
- explain how the following genotypic andphenotypic methods of microbe identification is done. Use diagrams or schematic representations to explain yourwork. (1) Restriction Fragment Length Polymorphism (RFLP)(2) Pulsed-Field Gel Electrophoresis (PFGE)(3) Whole Genome Sequencing (WGS)(4) Gram Staining(5) Biochemical Reactions: Multiple Tube Fermentation Technique(6) Biochemical Reactions: IMViC TestOf the two principal polymers used in column chromatography and electrophoresis, which one would be most immuneto contamination by bacteria and other organisms?You discovered that a species of bacteria can break down StyrofoamTM (polystyrene) products due to anenzyme it produces, polystyrenase. You wish to study the gene that codes for this enzyme.Task 1: DNA ExtractionTo begin work on the bacterium, you begin by extracting its genomic DNA (gDNA). What is the purpose ofthe following procedures? Answer briefly but completely.a. Using sodium dodecyl sulfate, a detergentAnswer: __________________________________ b. Adding RNase A and Proteinase K during extractionAnswer: __________________________________ c. Adding ethanol before recovering the DNA extractAnswer: __________________________________ Task 2: Polymerase Chain ReactionAfter purifying the gDNA extract, you want to isolate and amplify the polystyrenase gene. You perform PCRusing the appropriate gene-targeted primers. What is the purpose of the following PCR components? Answerbriefly but completely.a. DNA polymerase isolated from Thermus aquaticusAnswer:…
- 3) Define gel electrophoresis, including its theory and application. Describe the steps of running gel electrophoresis using the following image. More detailed reading: https://www.sciencedirect.com/topics/medicine-and-dentistry/agar-gel-electrophoresis POWER SUPPLY CATHODE ELECTROPHORETIC BUFFER ANODE WELL SAMPLE AGAROSE GEL POWER SUPPLY CATHODE ANOCE HIGH MOLECULAR WEIGHT SPECIES LOW MOLECULAR WEIGHT ANALYTESWhat advantage(s) does nucleic acid amplification have overstandard culture methods for identification of microorganisms?What are the disadvantages?You want to subculture your cells from T25 flask to 96-well plates. You first collected your cells in a tube with 5ml of culture medium. Then carried out a trypan blue assay and counted your cells with a hematocytometer as shown in Figure below. Answer the questions according to the results: a. Calculate the concentration of the stock including the dead and living cells. Dont forget to show the units! b. Calculate the percentage (%) of the living cells in the stock. c. You want to seed 6000 living cells into each well of 96 well plates, then calculate the volume you should take from the stock for each well. 輯 1 mm I IRE