What settings and time are normally used for sterilization in an autoclave?

Q: Match the most appropriate method of sterilisation for the following items. 10 mL vitamin solution 5…

A: Any physical or chemical process that deals with the elimination or removal of both spores and…

Q: The solution containing bacterial spores is heated in an autoclave. When the autoclave has reached a…

A: Given that          Number of spores after autoclave reaches 121 C (No) = 106 spores/ml…

Q: what is the definition of  Packed bed reactor (PBR)?

A: Introduction: The chemical industry is extremely vital to economies all over the world, as it is…

Q: what does chroomatography observation mean

A: Column fractionation was developed in the 1890s as a technique of separating the various components…

Q: Which characteristic of the species used determines whether sterility was achieved when autoclaved?

A: Microorganism: These are too small organism which is impossible to see by naked eyes. They exist in…

Q: Why Purified synthetic alcohol is preferred for level vial?

A: An alcohol is an organic compound that carries at least one hydroxyl functional group (-OH) bound to…

Q: In bacterial teansfomation, why is 42oC being used as the chosen temperature for  heat shock…

A: In bacterial transformation, heat shock is used to increase the efficiency of the transformation…

Q: What do you mean by ultrafiltration?

A: The excretory system is the system that performs excretion, the bodily process of discharging…

Q: Agar is a solidifying agent used in media preparation. What is its origin?

A: Agar gel is a polysaccharide which can become liquefied at temperatures ranges from 85°C to 90°C and…

Q: The steps to convert chaulmoogra oil into an injectable treatment are: 1. extraction II.…

A: The chaulmoorga oil was used to treat leprosy. In earlier times the extracted seed oil was directly…

Q: description of the growth and growth pattern of the microorganisms in the pour plate method?

A: What do you understand by the term pour plate method? Give a brief description of this method.. What…

Q: You are given reference temperature 121 °C, Z value 10 °C, F0 15 min for killing Clostridium…

A: Since the heat treatment of the food kills all microorganisms that might ruin the food and those…

Q: Disinfectant Concentration Growth after time of exposure (minutes) Control 5 minutes 10 minutes…

A: * Disinfectant is a chemical substance that inactivates or to destroy the microorganisms that are…

Q: What is the effect of moist heat and dry heat? Define TDT and TDP. What is the minimum sterilization…

A: Sterilization is defined as a technique which is used for complete removal, killing or deactivation…

Q: Why do the results for the confirmed test represent  faecal coliform in the analysis of water using…

A: MPN is Most probable number. It is commonly applied for testing the quality of water.

Q: The thermal death point refers to: 1) The time it takes to kill an organism in an autoclave 2) The…

A: As more and more microbes were discovered and identified as the cause of various infections or…

Q: The autoclave uses a combination of ___ to accomplish its function: a) temperature and pressure b)…

A: a) temperature and pressureExplanation:An autoclave is a widely used device in various fields,…

Q: Identify applications for ethylene oxide sterilization.

A: KEY WORDS :- Ethylene oxide - It is a gas which is flammable in nature and colorless which is used…

Q: How can a life cycle of microorganism and heat resistance graph be used to determine the boiling…

A: Heat is one of the effective as well oldest physical methods that have been employed for microbial…

Q: Under what conditions are media typically sterilized in an autoclave?

A: An autoclave is the pressure chamber used to carry out the scientific and industrial processes…

Q: Compare, contrast, and differentiate between medical and surgical asepsis. Which one is the best…

A: Asepsis:- it represents a condition in which disease causing pathogens are almost absent. It…

Q: ) Gel filtration chromatography on Sephadex G50 b.SDS-PAGE

A: Gel filtration: with this method we could separate  protein with respect to molecular weight in the…

Q: What are high level disinfectants? What is an example of one? What is the method of action and the…

A: Disinfectants are chemicals that are used to kill the microorganisms present in the surroundings.…

Q: What is the concentration of a 50% solution after completing three 1:10 serial dilutions?

A: Serial dilution is the process that reduces the concentration of a solution in an organised manner.…

Q: Aside from using the autoclave, what are other ways to kill microorganisms from glassware that can…

A: * Culture media has to be sterilize before using it. * Sterilization is a process that destroys or…

Q: What is the hydrodynamic stress of bioreactors when there are cell cultures?

A: Tissue culture is a discipline that originated from biomaterials development and referred to…

Q: Differentiate the following sterilization techniques: a. Autoclave b. Dry heat c. Sterile filtration

A: Sterilization is a methodology which involves complete removal of microbes so that such microbes…

Q: What is the use of hay infusion in determination of microbial motility?

A: Hay infusion use in determination of microbial motility.

Q: List five essential procedures of the aseptic technique when transferring a broth culture to another…

A: The term "aseptic method" refers to a combination of medical practises and procedures that help…

Q: What are some advantages and disadvantages of using ionizing radiation to sterilize food?

A: Sterilization involves physical methods for removing or controlling the microbes. The physical…

Q: Why do you think autoclave sterilization of media is preferred over open flame?

A: An autoclave is used to sterilize surgical equipment, laboratory instruments, media  and other…

Q: Of the two principal polymers used in column chromatography and electrophoresis, which one would be…

A: Column chromatography is a purification method that uses the polarity of compounds for the…

Q: What is differential medium

A: Differential medium or indicative media contain compounds which support the growth of microbes and…

Q: In autoclaving media and glassware, why do you need to maintain the temperature at 121 0C and…

A: Microbial culture is done in sterile environments. The media and glassware used in the process are…

Q: In pharmaceutical companies, Sterilization is an important step for quality assurance and approval…

A: Introduction  Microorganisms such as bacteria, viruses etc. are pathogenic in nature and many of…