Q: If a bacterial isolate shows intermediate to moderate resistance to an antimicrobial, how might this…
A: Bacteria are microscopic single-celled prokaryotes that thrive in diverse environmental conditions.…
Q: Why does a precipitate form on Sierra’s medium when inoculated with a lipolytic organism? Discuss…
A: Nutrient mediums are used to provide growth to the microorganism in invitro experiments. These…
Q: Why is dilution a necessary part of pure culture preparation?
A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…
Q: Why is Hydrogen peroxide solution so effective against anaerobes?
A: Hydrogen peroxide is a chemical compound that is acts as mild antiseptic. It is also an endogenous…
Q: What is sterilization? Give specific examples of media which are sterilized by the following…
A: All microbes, such as bacteria, virus, and fungi, requires some essential nutrients for growth. The…
Q: Why do cells need to be kept in the freezing devices first then transferred to liquid nitrogen…
A: Rapid freezing of cells can cause ice crystals large crystals can be formed in which cells are…
Q: Would an obligate anaerobe or an obligate aerobe be more likely to produce catalase? Why?
A: Introduction : obligate anaerobes are those bacterium that can live only in the environment…
Q: Can culture media be sterilized in the hot air sterilizer? Why or why not?
A: Hot air sterilization is a procedure in which the sterilization of the objects are done using hot…
Q: Why del factor is important in media sterilization?
A: Sterilization, which was called by various means such as Del factor, Nabla factor and the…
Q: Why is resazurin a useful media additive for the study of anaerobes?
A: Resazurin is a weakly fluorescent, non-toxic, redox-sensitive phenoxazine dye which has a…
Q: Define sterilization.
A: Microbial control indicates the prevention or inhibition of microbial growth. It involves physical…
Q: What steps are necessary to ensure the sterility of materialcontaminated with bacterial endospores?
A: During endospore formation, the bacteria protoplasm is condensed to a limited volume, which leads to…
Q: What is the effect of moist heat and dry heat? Define TDT and TDP. What is the minimum sterilization…
A: Sterilization is defined as a technique which is used for complete removal, killing or deactivation…
Q: what type of bacteria would be used to make sauerkraut?
A: Sauerkraut is product of cutting raw cabbage that fermented by various lactic acid bacteria. The…
Q: What concentration of agar is used in a semisolid medium for motility determination? How does that…
A: Agar is a polysaccharide that is obtained from marine seaweeds. Agar is mostly used in food and…
Q: Why are complex media preferable to chemically defined media for routinecultivation of…
A: Microorganisms require nutrients as an energy source and certain other environmental conditions to…
Q: What are the advantages of UV disinfection versus, or as acomplement to, chemical disinfection with…
A: UV disinfection treatment is a very rapid physical process for disinfecting or sterilizing any…
Q: What medium is this? What is the result in tube at left? In second tube? What does it tell you about…
A: One stab towards the bottom of the SIM tube is used to inoculate it. If the microorganism is motile,…
Q: Which microorganism does not contain superoxide dismutase enzymes?
A: Superoxide dismutase (SOD) is an enzyme found altogether in living cells. An enzyme could be a…
Q: Why is PBS buffer preferred when preparing a monolayer cell culture?
A: PBS (phosphate buffer saline) is a buffer solution which is a water based salt solution containing…
Q: What is the effect of trypsin EDTA solution on monolayer cell culture preparation?
A: Trypsin is an enzyme which helps in digestion of proteins.In the small intestine,proteins are broken…
Q: Why is it desirable that most cultures be inspected after 15 to 18 hours of incubation?
A: Introduction Microbial culture is a microbiological technique that involves actively multiplying…
Q: What settings and time are normally used for sterilization in an autoclave?
A: The fundamental rule of steam sterilisation as cultivated in an autoclave, is to open every thing to…
Q: Why do you think autoclave sterilization of media is preferred over open flame?
A: An autoclave is used to sterilize surgical equipment, laboratory instruments, media and other…
Q: Of what practical importance are air borne microorganisms to the laboratory workers? What…
A: Questions related to the microbiology labs. Note: each questions are answered under particular…
Q: Why are most antimicrobial chemical agents disinfectants rather than sterilants? What general…
A: Most chemical antimicrobial agents are disinfectants instead of sterilants. A disinfectant reduces…
Q: Why would cells that are vigorously growing when inoculated into fresh culture medium have a shorter…
A: Growth curves are generally the description of the density of cell populations in liquid culture…
Q: what is sterilization or define sterilization?
A: Sterilization is defined as the process where all the living microorganisms, including bacterial…
Q: What are the possible ways to sterilize heat labile products? Explain your answer with suitable…
A: Heat labile materials are those substances that can undergo change rapid change when exposed to high…
Q: How does an autoclave sterilize instruments? What is used to dry glassware and at the same time
A: Autoclaving is the best method for sterilizing glass instruments. Sterilizing means removal of all…
Q: In determination of microbial motility, what is the value of a hanging-drop perparation? what is the…
A: Hanging drop is a motility testing which helps microbiologist to view motility of bacterium.
Q: What is the point of the pressure in the autoclave sterilization?
A: Autoclaves are the devices which are like pressure cookers and used in laboratories that provide a…
Q: Of what practical importance are air borne microorganisms to the laboratory workers? What…
A:
Q: What is the most difficult type of microbial cell or structure to kill? What methods can be used to…
A:
Q: How can organisms develop resistance to chemical disinfectants?
A: Bacteria can develop resistance to disinfectants over time either by acquisition of exogenous mobile…
Q: How does smear preparation of cells from a liquid medium differ from preparation of cells from a…
A: Answer: Smear Preparation is the method in microbiology laboratories to place the small amount of…
Q: Why is egg enrichment not added to BPA prior to sterilization?
A: Baird–parker agar or BPA is a selective medium is identify or isolate S. aureus in food. It contains…
Q: Why are plated media stored in an inverted position?
A: Plated media enables the isolation of microorganisms from samples or specimens.
Q: What is the purpose of flaming an inoculating loop?
A: The question asks to give the reason for flaming or sterilizing an inoculating loop.
Q: What is the hazard of the splattering tendency in flame sterilizing an inoculating loop? Can…
A: Aseptic preparation of culture media :-
Under what conditions are media typically sterilized in an autoclave?
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- Why is PBS buffer preferred when preparing a monolayer cell culture?In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either. Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-mL graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-mL beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropperIdentify the function of the following enzymes: Superoxide dismutase (SOD)
- An autoclave is typically used for sterilization of media. a. Define sterilization. b. Under what conditions are media typically sterilized in an autoclave? c. What type of media components cannot be sterilized in an autoclave? d. What is an alternative to autoclaving for sterilizing heat-sensitive materials?What advantages do solid media offer for the isolation of microorganisms? How will you check the purity of an isolate?In an autoclave, how are temperatures above boiling achieved? (Answer in not more than 2 sentences)
- A disk-diffusion test using E. coli gave the following results: Disinfectant Zone of inhibition (diameter in mm) A 15 B 2.0 C 10 D 5 In the table above, which compound was the most effective against E. coli? A) A B) B C) C D) D E) The answer cannot be determined based on the information provided.Why does motility media contain a lower amount of agar than ordinary agar?Define sterilization.