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- Consider the photos here which demonstrate antibiotic sensitivities of Staphylococcus aureus strains as determined by the Kirby-Bauer method. Abbreviations are as follows: C = chloramphenicol; CC = clindamycin; CZ = cefazolin; E = erythromycin; NOR = norfloxacin; OX = oxacillin; P = penicillin; RA = rifampin; SAM = sulbactam-ampicillin; SXT = sulfatrimethoprim; TE = tetracycline; VA = vancomycin. Imagine that only two cellular changes occurred in the original strain (the first image, on the top) that resulted in the resistance pattern of the strain in the second image (on the bottom). Which combination of mechanisms could explainthese results?Choose one or more: A.expression of efflux pumps B.overproduction of PABA C.production of β-lactamase D.altered penicillin-binding protein E.modification of either 50S or 30S ribosomal subunits F.altered DNA gyraseWhich of the following statements is correct about column chromatography? a) Resolution improves as column length increases b) Mobile phase is a porous solid material kept in the column with appropriate chemical characteristics c) Stationary phase is a buffered solution that percolates through the mobile phase d) Large proteins exit the column faster than tiny proteinsWhat is meant by recalcitrance in microbiology? Give an example
- In step 3 of today's protocol, it mentions that the incubation step with the P2 buffer should not be allowed to proceed for more than 5 minutes. a) What is the chemical composition and concentration (not mass) of each component of the P2 buffer?b) c) What is the purpose of adding the P2 buffer? What would be the major problem if this step is allowed to proceed for a long period of time?Discuss the different methods for RBC preservation including their corresponding purposes a) Rejuvenationb) Freezingc) Anticoagulants and additive solutionsCaffeine is used in bilirubin assays to: 1) Stop the diazo reaction 2) Accelerate indirect bilirubin reaction 3) Accelerate direct bilirubin reaction 4) Precipitate proteins 5) Reduce sodium nitrite no references, just homework
- Write down a comparison of the sizes of the untreated Allium cepa cell and the colchicine-treated Allium cepa cell.Name the enzyme which is used as clot buster” to remove blood clot from blood vessels of patients.Patch clamp What is patch clamp technique? Identify two features of the patch clamp technique that make it a useful tool in studying cellular electrophysiology. Explain each thoroughly. What is the role of the reference electrode in the patch clamp technique? Discuss how it works.
- https://courses.lumenlearning.com/microbiology/chapter/controlling-microbial-growth/ Read this Article about Controlling Microbial Growth and answer the following questions: Which is most effective at removing microbes from a product: sanitization, degerming, or sterilization? Explain. What are two possible reasons for choosing a bacteriostatic treatment over a bactericidal one? Name at least two factors that can compromise the effectiveness of a disinfecting agent.-For each item listed below, select a sterilant that you feel is the most suitable and effective for that item. Discuss why you selected each sterilant.? A) plastic Petri dishes to be used for nutrient media B) glass tubes of bacterial cultures to be discarded C) sterile milk D) dozens of reusable glass pipets E) bone tissue for grafting F) room air G) hospital mattresses H) biopsy tissue for histology analysis I) plastic respiratory therapy equipment and arthroscopes J) vaccinesWhat chemical is utilized as the reagent known as VP-B?