What are the disadvantages of biochemical test?

What is biocompatibility? Briefly describe how the success of a biomaterial is evaluated.

Which of the following are true regarding assay attributes? List all that are true. a) Accuracy represents the closeness of the assay result to the “true value”. b) Robustness represents the ability of a method to distinguish between the analyte and similar components. c) Precision is determined by replicate analysis of a reference standard or well-characterized material. d) A robust assay would have a very narrow range of acceptable assay conditions.   For those that were not true in question 1, correct the statement(s).

Explain the basis for identification using biochemical testing. – Again, discuss enzymatic pathways, how do we make enzymes in a cell, what is the blueprint for an enzyme? Please keep this answer to 2-3 sentences

What is the purpose of a standard curve for a colorimetric enzyme assay?

Describe technical, analytical and clinical validity of clinical biochemical tests.

If you are using sensitivity and specificity to evaluate how well a dichotomous test (like a field sobriety test or home pregnancy test) is working, ideally what do you hope to see? Choose the correct answer and explaing why:     a) Low specificity and low sensitivity.            b) High specificity and high sensitivity.            c) Low specificity and high sensitivity.            d)_High specificity and low sensitivity.

After adding stop solution (sulphuric acid) in reaction mixture of HRP assay with TMB, yellow color becomes brighter. What is the mechanism?

Name some techniques/approaches (name, describe the major steps, how to analyze/interpret results)  that can be used to satisfy the following requirements:  1. Show that substance is pure protein 2. Only one protein was extracted

Why do we use standard solutions in protein assays? Explain briefly.