In a fed-batch culture operating with intermittent addition of lactose solution, values ofthe following parameters are given at time t = 2, when the system is at a quasi-steady state. 1.Determine the initial volume of the culture 2. Determine the concentration of growth-limiting substrate and the total amount ofbiomass in the vessel at a quasi-steady state. 3. At which scenario of bioprocessing, a fed-batch system is recommended to be applied?

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Metabolic pathways

Metabolism is defined as the group of life-supporting, enzyme-mediated chemical reactions in organisms. A metabolic pathway is a set of these chemical reactions that involve anabolism (synthesis of complex biomolecules) and catabolism (breakdown of biomolecules into small molecules) that occur inside a cell. Metabolites are the reacting substances, products, and intermediate substances of an enzymatic process that are altered by a series of chemical events catalyzed by enzymes.