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- Given this, if you used 6g of vitamin Z powder to make 20 ml of solution, what is the % concentration of this solution? (I gave the image since I don't know if that info is needed to solve this question.)It also gives a follow-up, if you can help here too: You work in a lab as a summer student. One of your tasks is to make sure that there is enough cell culture medium containing antibiotics to grow bacteria. One day you realize that there is only 5 ml of 10% Antibiotic stock solution in the freezer. You decide to use it all to prepare the working culture medium with 0.01% antibiotic. In the lab there is plenty of growth medium without antibiotics. (Note: dilution in medium is like dilution in water). You remember the equation to make dilutions of stock solutions. You usually use this formula to calculate the required volume of a stock solution, but you realize it can apply here as well, even though the unknown is the final volume. So, you make that dilution. Given that each bacterial…Explain this approach: matrix-assisted laser desorption ionization (MALDI) ?Describe two different applications of superparamagnetic particles in bio-medicines and what are the advantages of using superparamagnetic particles.
- Nanoparticles were added in a liquid to prompt the medication release, and it was observed that 5 mg of the medication was released in 1 liter of PBS, which accounted for 60% of the total amount of the drug. While producing an equivalent number of nanoparticles, 5 ml of 10 mg ml−1 of the drug was added. What is the efficiency of encapsulation?Immunofluorescence and Fluorescent livecell imaging techniques can both be used to determine protein localisation.List the advantages and disadvantages of using immunofluorescence andfluorescent live cell imagingDescribe the bioreactor design consideration to be taken in account during the bioprocessing of animal cell culture. Please discuss which factors influence the choice of bioreactor design?
- Briefly outline &/or sketch the premise of the transwell assay:a) List three biomaterials that are used in cancer diagnosis and therapeutic.b) List three biomaterials that are used in scaffold applications.Differentiate between surface immobilisation and microencapsulation of enzymes and discuss the advantages and disadvantages of each method.
- Describe various detection methods used in High-Performance Liquid Chromatography (HPLC) and types of biomolecules that are detected in each method?Define about sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)? Explain importance of sodiumdodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ?Explain the key concepts of a) Bioavailability b) Protein binding c) Therapeutic index