Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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- Are you a hidden heterozygote? A PCR analysis (part2) Agarose gel electrophoresis and interpretation la: Several factors (including agarose gel concentration, time and current) affect migration of DNA fragments through the agarose gel. Briefly explain how each of these factors affects DNA migration. Agarose gel concentration: Time: Voltage: 1b: Do DNA fragments move towards the positive or negative end of the gel box? Explain your answer. 1c: What is the purpose of the Tris-Acetate-EDTA (TAE) buffer that the agarose gel is prepared with and submerged in for running? What would happen if you used water to prepare and run the gel instead of TAE buffer? 1d: If the student is homozygous for the brown allele, how many bands will they see in the lanes for the blue and brown allele samples? (circle one) Brown sample: 0 Blue sample: 1 2 more than two. 1 2 more than two. le: If the student is homozygous for the blue allele, how many bands will they see in the lanes for the blue and brown allele…arrow_forwardQUESTION 5: Should PCR primers be complementary to each other? Explain the reasoning for your answer.arrow_forwardWhat is one of the end results of site-specific recombination? Diagram your answer and answer in 1-2 sentences.arrow_forward
- I’m having trouble finding which answer would be acceptable can you please help?arrow_forwardParts a-earrow_forwardA 2.0kb bacterial plasmid ‘BS1030’ is digested with the restriction endonuclease Sau3A; the plasmid map is depicted in the diagram below and the Sau3A (S) restriction sites are indicated. Which of the following DNA fragments do you expect to see on an agarose gel when you run Sau3A-digested plasmid ‘BS1030’ DNA? a. 250 bp, 450 bp, 550 bp, 1.1 kb, 1.5 kb and 2.0 kb b. 2.0kb c. 250 bp, 400 bp, 450 bp, 500 bp and 550 bp d. 100 bp, 200 bp, 250 bp, 400 bp, 500 bp and 550 bparrow_forward
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- Question 1: Look at the following normal and mutant DNA sequences. Normal Sequence (5'-3'): ATG AAC GTT ATC GCA Mutated Sequence (5'-3'): ATG AAT GTC ATC GCA a) What type of mutation has occurred (be specific)? b) Fill in the table for the normal and mutated sequences. Starting with the given 5'-3' sequence, input the complementary DNA, transcribed RNA from the 3'-5' DNA and translated polypeptide sequence for both. Hint: use the codon table! Normal sequence Mutant sequence DNA 5'-3' (given) DNA 3'-5' RNA Polypeptide (use 3 letter codes) c) Based on parts A and B above, what is the ultimate effect this mutation has had on the polypeptide? (1 sentence summary)arrow_forwardQuestion 2. For your senior research, you end up studying the life cycle of an animal virus whose genome consists of a single circular, double-stranded DNA molecule. Your project is to define the number and location of the origin(s) of replication and to determine whether replication proceeds in one (unidirectional) or both (bidirectional) directions away from an origin. To accomplish this, you isolate many identical strands of DNA that have already been partially replicated. You cleave each piece of DNA exactly once with a restriction enzyme. You then observe the cut pieces of DNA using an electron microscope. Below is a schematic representation of what you observe. Remember that each line represents a different piece of DNA, and not a fragment of a larger piece. Using this data, answer the following questions: A) How many origins of replication do you think the viral genome has, and why do you think this? 30: F2 #3 *** E D 80 F3 54 $ 4 F4 R LL % 5 F5 T G B) Do your data support…arrow_forwardThe region of the normal hemoglobin gene used for genetic testing for sickle cell anemia contains a restriction site such that homozygous normal individuals show two DNA fragments. If a single nucleotide change in hemoglobin destroys that restriction site, then how many DNA fragments will be visible on a gel from individuals that are homozygous mutant? What about heterozygotes?arrow_forward
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