QUESTION 18 Which of the following is/are true regarding the enzyme PRIMASE? Primase functions during cellular DNA replication Without primase activity, DNA polymerase in our cells can NOT begin synthesizing new nucleotide chains Primase uses DNTP building blocks Primase is a polymerase enzyme Primase functions AFTER DNA helicase activity

Question 10. What basal transcription factors bind to the DNA within the pre-replication complex immediately after TATA-binding protein (TBP) bends the DNA? Short Answer 140

Question 10 Produce the complimentary DNA strand that would be matched with the provided strand T--A C-- G C-- G A -- A -- C-- G-- T-- T-- A -- C-- G -- G--

Question 16 Why can't SNPS be detected by PCR and Gel Electrophoresis? O Because Gel Electrophoresis detects size differences in DNA and SNPS do not change the size of the DNA strand. O Because SNPS cause deletions so large that they are beyond the limits of this technique to detect. O Because SNPS affect proteins and PCR only works on DNA. O Because SNPS are too complicated to detect with this technology.

QUESTION 6 These correct “overwinding” ahead of the replication forks by breaking, swiveling, and rejoining DNA strands.     Helicases     Single-strand binding proteins     Topoisomerase     Telomerase

Question 11 During mitosis the chromosomes line up in the middle of the cell are copied all of the above are wound up on histone spools to keep them from tangling are pulled apart by molecular workers

Question 2. For your senior research, you end up studying the life cycle of an animal virus whose genome consists of a single circular, double-stranded DNA molecule. Your project is to define the number and location of the origin(s) of replication and to determine whether replication proceeds in one (unidirectional) or both (bidirectional) directions away from an origin. To accomplish this, you isolate many identical strands of DNA that have already been partially replicated. You cleave each piece of DNA exactly once with a restriction enzyme. You then observe the cut pieces of DNA using an electron microscope. Below is a schematic representation of what you observe. Remember that each line represents a different piece of DNA, and not a fragment of a larger piece. Using this data, answer the following questions: A) How many origins of replication do you think the viral genome has, and why do you think this? 30: F2 #3 *** E D 80 F3 54 $ 4 F4 R LL % 5 F5 T G B) Do your data support…

QUESTION 6: Based on your knowledge of DNA replication, indicate the polarity of each primer.

QUESTION 2 Which of the following would be classified as non-coding DNA? (mark all applicable answers) The cytochrome oxidase Il gene found on the human mitochondrion Introns of the alpha hemoglobin gene found on human chromosome 16 Exons of the alpha hemoglobin gene found on human chromosome 16 U The DNA between genes in the human genome (also referred to as intergenic regions or IGRS)

Question 3