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- a) Describe the technique Of Atomic Force Microscopy under the following headings: (i) Basic principle of AFM and overview of operations (ii) Modes of A FMD) When performing fluorescence microscopy what are the stokes shift and why is it better to have fluorochromes with a large stokes shift? E) What is photobleaching and what is done when imaging histological samples to overcome it when performing fluorescence microscopy?Assume you are observing the diatom pictured in Figure 1 using the 10X lens in a compound light microscope. You move to the 40X lens and then again to the 100X lens by only rotating the turret (remember that the lenses are parfocal), without making any other adjustments to the microscope. c) After making your adjustments, you notice that the midline of the diatom is in focus while the remainder is blurry. Explain, based on microscopy principles, why this has occurred Provide a description of the procedure to prepare an acidic stain of bacteria using Nigrosin as it would appear in the Methods section.
- What is phase contrast? Give examples of phase contrast in optical microscopy.On a particular microscope, the numerical apertures of the condenser and the high dry power objective lenses are 1.25 and 0.65 respectively. You are supplied with a filter that selects a wavelength of 500 nm. A) What is the limit of resolution on this microscope when using the high dry power lens? (show your calculations) B) Will you be able to distinguish two points that are 260 nm apart as being separate or will they blur into one? (explain your answer)It is desirable that the microscope objectives be parfocal because the specimen will be .................. a)blurry due to the changes in the refractive index of the medium b)stay in focus when the light is turned off c)have more contrast and enhanced details d)remain in focus if the magnification is changed e)none of the answer choices
- The following is an UV-Vis schematic diagram. Based on this diagram, answer the following questions: D2 Lamp (B) Detector Light Source (A) Sample What are the components labelled as (A) and (B)? Briefly explain the functions of them.You have a microscope equipped with 10 x oculars and 4 objectives, which exhibit the following characteristics (M, NA): A=(4 x/0.13); B=(20 x/0.4); C=(20 x/0.7); D=(40 x/0.65) Tick the right answers: o Objectives B and C are equivalent. o Objective D will allow more detailed observation than objective C. o To see small structures, you should choose objective D rather than C. o Objective D has a lower resolution than objective C. o To observe large objects, it is better to use objective B rather than objective CGiven the following summary of the two microscopes, identify the microscope to be used in these scenario: 1. An image with 18x actual magnification and an apparent size of 1.04mm 2. Unknown specimen that is 0.435 angstrom 3. A microorganism with 2.1 nm size *show solution and brief explanation
- How will the following affect resolution during microscopy? I) Closing or opening the diaphragm II) Raising or lowering the condenser III) Increasing or reducing the light intensityExplain briefly why the 100X objective lens of the light microscope is immersed in oil.Explain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron, and scanning electron microscopy for a given situation. What is an example of this situation?