a) Describe the technique Of Atomic Force Microscopy under the following headings: (i) Basic principle of AFM and overview of operations (ii) Modes of A FM

Q: Why should the microscope be calibrated for each objective and prior to each use?

A: Introduction  Calibration is the process of establishing up an instrument such that it can produce a…

Q: An opaque object requires _________illuminationfor viewing with a microscope

A: A microscope is the most common laboratory instrument. It is primarily used in object examination…

Q: Table 1 - Characteristics of the microscope Low Power (Dry) High Power (Immersion Oil) Features…

A: A microscope is a device that allows you to examine tiny objects, including cells. The picture of an…

Q: Differentiate between the limit of resolution of the typical light microscope and that of the…

A: The microscope is an instrument that helps us to visualize the objects that cannot be seen by our…

Q: Compare bright-field, dark-field, phase-contrast, confocal, andfluorescence microscopy as to field…

A: Optical microscopes use light source that consists of a band of colored wavelengths in the visible…

Q: What particular features of fluorescence make it useful for microscopy?

A: Step 1 Microscopes are an instrument which is used for observing small objects and finer internal…

Q: What are the different difficulties faced in microscopy?

A: Microscopy is the field of science which uses microscope for the study of microstructures which are…

Q: Describe the process of Scanning Confocal Microscopy?

A: Microscopy allows magnification of structures to visualize them.

Q: high-resolution image in an optical microscope?

A: Resolution is the fineness and detailing of the picture and it is measured in pixels per inch…

Q: If a microscope has a 40x objective lens in place and a 15x ocular lens, the total magnification of…

A: Microscope is used to see tiny objects in enlarged size to study the internal mechanism of object.…

Q: Why is CSLM able to view different layers in a thick preparationwhile bright-field microscopy…

A: The technical field that uses microscopes to observe objects and their areas that cannot be seen…

Q: and contrast the source and benefit of reporting microscopy findings for a direct smears versus…

A: A microorganism smear is a thin layer of microorganism that is stained on a slide. Preparing the…

Q: discuss the microscopy technique that gives the best constrast image of non biological samples such…

A: Microscopy is an instrument used to magnify the size of the objects or particle which cannot be seen…

Q: Compare the appearance of microorganisms you observed using phase-contrast microscopy versus…

A: Microscopy is a basic form of microscopic technology. It depends on the interaction between light…

Q: a.  Why must you slowly turn the fine adjustment knob? b.  Is natural light or is artificial light…

A: The optical magnifying instrument, additionally alluded to as a light microscope is a sort of…

Q: What is the usage of the superresolution microscopy technique?

A: The microscope is identified as an optical instrument to observe very small objects. It is able to…

Q: Explain the basic differences between fresh and fixed preparationsfor microscopy and how they are…

A: In microscopy, the preparation of a specimen involves the mounting of a sample on a suitable glass…

Q: What is the the total magnification of a microscope that is set up using a 10x ocular lens and a 20x…

A: A microscope is a scientific tool that is used to look at organisms or things that can't be seen…

Q: What is the difference between a phase-contrast and differential interference-contrast microscope?…

A: Microscope is an instrument that gives large images of small objects, This allows an exceedingly a…

Q: For which types of specimens is darkfield microscopy preferred over brightfield microscopy?

A: Microscope is an instrument used to view objects and organisms that are too small to be seen by…

Q: riefly describe the functions of the following parts of the microscope: course objective knob,…

A: Microscopy is the technique of using a microscope and it demands an understanding of its working and…

Q: Which of the following statements are true in the context of the microscope's field of view? I. It…

A: Microscope field of view (FOV) It is defined as the maximum area visible when looking through the…

Q: Compare bright-fi eld, dark-fi eld, phase-contrast, confocal, and fl uorescence microscopy as to fi…

A: Microscopy allows one to view samples and objects that cannot be seen with the human eye. With all…

Q: a. How was the specimen prepared for the microscopy technique applied? (for e.g. stained with H&E…

A: As per the guidelines, we are supposed to answer only three sub-parts. Kindly repost the question…

Q: What is the purpose of confocal microscopy technique ?

A: The microscope instrument is used to view the objects, which are not visible under the naked eye.…

Q: Differentiate the Bright-field and a Dark-field microscopy.

A: Microscopy -- The word microscopy is derived from the Greek word  micros - small Skopeo - to view…

Q: 3D dimensionality is a limitation of the compound microscope. Depth of field, DOF, describes…

A: The compound microscope provides a 2-dimensional image of the specimen as there is just one path for…

Q: What adjustments can improve a microscope's resolution?

A: Resolution or RP is the capacity of an optical system to distinguish two closely placed objects from…

Q: What advantages do confocal and deconvolution microscopy provide in comparison to conventional…

A: Confocal microscopy uses light from laser, elimination of background information away from the focal…

Q: otal magnification of a microscope is determined by Multiple Choice dividing the objective…

A: The tiny and minute organisms that cannot be observed by unaided eyes are the microorganisms. These…

Q: What are the dehydrating agents used for Electron Microscopy, and why?

A: Dehydration in microscopy is a technique of removal of water content from the sample specimen and…

Q: What are the advantages of using super-resolution microscopyversus standard fluorescence microscopy?

A: Fluorescence microscope is a type of microscope that makes use of phosphorescence and fluorescence…

Q: a. b. What is the basic principle of image formation using this microscopy technique? What can be…

A: There are few important points : Scanning electron microscopy : It is very powerful and versatile…

Q: Describe the conditions under which dark-field microscopy is the best choice for viewing specimens.

A: Darkfield microscopy is a method of microscopy in which the images are excluded from the unscattered…

Q: Why is it important to center your object before moving to higher magnifications in a light…

A: A microscope is a crucial tool in the study of organisms that are very minute or to study the cells…

Q: Differentiate bright-phase and dark-phase microscopy

A: In the course of history of biology, various new tools and techniques have been developed to explore…

Q: Using a compound microscope, if I move my sample from up to down (1), in which direction will the…

A: Answer: COMPOUND MICROSCOPE = It is the microscope used light as an illuminating source and used to…

Q: Explain the differences between Brightfield, Darkfield, and Phase-Contrast Microscopy.

A: * Here are some differences between bright field microscopy darkfield miccroscopy and phase contrast…

Q: Distinguish scanning tunneling from atomic force microscopes in terms of how they create images and…

A: The optical microscopes which use visible light and lenses for magnifying images have disadvantages…

Q: What is the importance of resolution, magnification and contrast in microscopy

A: The lenses of the microscopes create two types of images when a specimen is placed beneath the…

Q: Consider the following microscope: ocular power = 10X low power objective = 20X high power objective…

A: A microscope is used to magnify and see such objects that cannot be seen by naked eyes. The process…

Q: How is confocal scanning laser microscopy differentfrom fluorescence microscopy? In what ways are…

A: Microscope is an analytical instrument which is used to analyse, study and visualize internal…

Q: What is the formula used to describe the resolution of a microscope lens, and what are the…

A: The term resolution is used in microscopy to characterize a microscope's capacity to identify…

Q: Transmission and scanning electron microscopy differ because transmission electron microscopy shows…

A: As per the guidelines, we are supposed to answer only one question. Kindly repost the other question…

Q: Describe 3 simple ways of preparing slides for light microscopy microscope

A: Introduction The optical microscope, often known as a light microscope, is a type of microscope that…

Q: Differentiate between the principles of light microscopy and the principles of electron microscopy.

A: Microscope helps to observe the organisms or objects that can not be seen by our naked eye because…

Q: What is the principle of image formation on transmission election microscope and scanning electron…

A: The general biological microscope helps in the visualisation of the image of a particular sample…

Q: How does differential interference contrastmicroscopy differ from bright-field microscopy?

A: Microscopes are used to observe objects at cellular level such as the nucleus, mitochondria and…

Q: Always begin examing microscope slides with which power objective?

A: A microscope is an instrument of immense importance in a biology/ microbiology laboratory. As the…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.